Quantification of regional myocardial blood flow in vivo with H215O.

Abstract

Using H,15O (half-

Residual coronary reserve despite decreased resting blood flow in patients with critical coronary lesions. A study by technetium-99m human albumin microsphere myocardial scintigraphy.

“…Because of their entrapment before the capillary network, the amount of microspheres (and then of radioactivity) taken up by the myocardium is Mup=CBFxCaxt (15) where Ca is the arterial concentration of labeled microspheres in the time t. Inasmuch as we used a bolus injection, Equation This method does not imply the problematic measurement from of the actual injected dose but presents two problems. 1) With a planar imaging device such as the mobile gamma camera, it is not possible to measure the radioactivity (and (8) then microsphere) concentration in the arterial blood; instead n be it is allowable to measure the amount of radioactivity present in each time t in a district of unknown dimensions.…”

A convenient route to [68Ga]Ga-MAA for use as a particulate PET perfusion tracer

“…Human serum albumin microspheres (Rhodes and Bolles, 1975;Davis, 1975) labeled with 68 Ga have found use in PET studies of pulmonary perfusion (Chester, et al, 1975;Mintun, et al, 1986;Schuster and Green, 1987), as well as in studies to validate freely diffusible PET markers of tissue perfusion (Schelbert, et al, 1980;Bergmann, et al, 1984;Steinling, et al, 1985;Mintun, et al, 1986;Schuster and Green, 1987). Albumin microsphere labeling with 68 Ga can be effectively achieved either by hydrolysis and precipitation of the 68 Ga 3+ ion in the presence of the albumin particles (Chester, et al, 1975;Hnatowich, 1976;Yvert, et al, 1979;Hayes, et al, 1981;Maziere, et al, 1986;Mintun, et al, 1986;Schuster and Green, 1987), or by covalent conjugation of the microspheres with a high-affinity gallium chelating ligand (Wagner and Welch, 1979).…”

Quantitative analysis of coronary endothelial function with generator-produced 82Rb PET: comparison with 15O-labelled water PET

“…Thus, 15 O-labeled water is considered the gold standard for non-invasive MBF quantification [33]. We have also applied this approach in the risk assessment in smokers [13] and also applied the assessment of therapeutic interventions [4].…”