Quantification of Salivary Nitric Oxide in Patients with Fixed Orthodontic Treatment

Abstract

Nitric oxide (NO) is considered a regulator of bone response to mechanical stress that mediates adaptive bone formation, the pathological effects of lipopolysaccharides (LPS), tumour necrosis factor (TNF), interleukin 1 (IL-1) and other cytokines; regulates leukocytes and epithelial cell adhesion; inhibits T cell proliferation; and enhances natural killer (NK) cell activity, as well as other immune-related processes. The aim of the current study was to test the potential use of salivary NO as a biomarker of bone response that is specific and sensitive to local changes, following the application of different types of dental appliances. Material and methods: Salivary NO was determined in 30 patients divided into three groups with 10 participants each: control (C), fixed metal braces group (M), and aligners group (A). Salivary NO was determined four times in each group (before the procedure, at 2 weeks, 30 days, and 60 days after the procedure) using ELISA and rapid semi-quantitative assay with Nitric Oxide Saliva Test Strips (Berkeley, CA, USA). The mean results were compared with the ANOVA test, and the Pearson correlation index was calculated. The results show a significant increase in salivary NO levels by both methods only in the metal braces group, which is suggestive of oxidative damage, increased invasiveness, and bone response to metal braces. In conclusion, our study showed that metal brackets lead to a significant temporary increase in oral oxidative stress as an adaptive reaction to the presence of foreign bodies in the oral cavity. The subsequent concentration decrease at 60 days suggests a normalization of the body’s response to foreign bodies.

Special Issue on New Techniques, Materials and Technologies in Dentistry

“…In another field, Raducanu et al [3] tested the potential use of salivary nitric oxide as a biomarker of bone response following the application of different types of orthodontic appliances. The results showed that metal brackets lead to a significant temporary increase in oral oxidative stress as an adaptive reaction to the presence of foreign bodies in the oral cavity.…”

Enzyme mimetic electrochemical sensor for salivary nitrite detection using copper chlorophyllin and carbon nanotubes-functionalized screen printed electrodes

“…Recent advancements in materials technology and additive manufacturing have led to the creation of smart sensing strips for salivary nitrite measurement, which are disposable and inexpensive. For example, Berkeley Life NO Test Strip (https:// www.berkeleytest.com) 23 is a commercially available disposable strip which monitors the nitrite levels in human saliva samples. Similarly, Bartovation NO 2 À strips (Bartovation.com, New York, USA), and HumanN NO strips (https://humann.com) are currently available in the market for personalized salivary nitrite measurement.…”