Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MS n -Based Identification

Elemental and Isotopic Mass Spectrometry

“…A number of research groups have analyzed these supplements as well as natural sources of selenium to determine the species of selenium present, and others have studied to elucidate the mechanisms by which selenium is metabolized and excreted [146,147]. to be speciated to such an extent for selenium including γ-glutamyl-Se-methylselenocysteine, a highly potential selenium species, which makes this bean material an ideal candidate for functional food purposes [148]. Work performed thus far has identified the amino acids selenomethionine, selenoethionine, and selenocysteine as likely cancer-preventative species, although a number of selenoamino acid derivatives, selenium-containing glutathione derivatives, and smaller organoselenium species have been detected and identified in selenium-enriched yeast [116].…”

Advanced Mass Spectrometry

“…The quadrupole is operated as an ion guide in MS mode and as mass selection device in MS/MS mode. A reflectron TOF analyzer is placed orthogonally to the quadrupole and serves as a mass resolving device for both MS and MS/MS modes [48][49][50][51][52][53][54][55]. A collision cell is located between the quadrupole and the TOF analyzer to induce fragmentation in MS/MS experiments [56][57][58][59][60][61].…”

“…Proteomic approach to detect powdered milk in fresh cow's milk [114] MALDI-TOF-TOF Proteins as targets of ferrous-catalyzed oxidation: identification of protein carbonyls [37] LC-TOF Evaluation of the immunostimulating activity of soybeans via the determination of the functional peptide soymetide [115] SELDI-TOF Effect of NaCl and sucrose tastants on protein composition of oral fluid [44] MALDI-TOF Hydrolysis of β-lactoglobulin by trypsin under acidic pH [116] UHPLC-TOF Quantification of Se-methylselenocysteine and its γ-glutamyl derivative from naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) [50] MALDI-TOF Detection of sheep and goat milk adulterations by direct MALDI-TOF-MS analysis of milk tryptic digests [117] CE-TOF Identification of bioactive peptides in hypoallergenic infant milk formulas [58] UHPLC-QqTOF Differentiation bovine and porcine gelatins in food products [118] [120]…”

“…LC-(Qq)TOF-MS has the ability to record an unlimited number of compounds operating in full-scan mode, which makes this technique very convenient for the development of screening strategies based on the use of accurate mass databases with automated librarybased detection. Another application of LC-TOF-MS and LC-QqTOF-MS/MS for pesticide residues analysis in fruits and vegetables is the identification of pesticide transformation products and metabolites, making use of the great advantage of having the possibility to identify and confirm with one single system [49,50].…”

Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

“…The monomethylated selenium compounds have been identified in various selenium-enriched vegetables, such as garlic, onion [ 29 ], broccoli [ 30 , 31 ], green bean [ 30 ], and in the sprouts of various species [ 32 ]. Anticancer and antioxidant properties of these Se-enriched vegetables have also been evaluated [ 10 , 33 , 34 , 35 , 36 , 37 , 38 ].…”