Quantification of silver ion release, in vitro cytotoxicity and antibacterial properties of nanostuctured Ag doped TiO2 coatings on stainless steel deposited by RF magnetron sputtering

Jamuna-Thevi, K.; Bakar, Syazana Abu; Ibrahim, Suhaina Mohd; Shahab, Neelam; Toff, M. R. M.

doi:10.1016/j.vacuum.2011.06.011

Cited by 154 publications

(96 citation statements)

References 22 publications

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“…By sputtering in a reactive environment (O 2 ), the intention was to enhance both bioactive and antibacterial properties of the coating, as formation of TiO 2 facilitates osseointegration by providing chemical stability and increased wettability [16,19,20,43], and incorporation of oxygen in silver is known to affect its dissolution rate [44]. The concurrent, reactive sputtering in this instance made for a compositional Ag-Ti oxide gradient with a dominant crystalline contribution from Ag.…”

Section: Discussionmentioning

confidence: 99%

“…In controlled amounts however, it presents little or no cytotoxic effects [14][15][16]. Incorporating silver in coatings with well-known biocompatibility and bioactivity, such as hydroxyapatite (HA) [17,18] or TiO 2 [16,19,20], has thus been presented as a viable option to reduce surgical site infection related to orthopedic and dental implants, while still maintaining good osseointegration properties. As a non-antibiotic agent with low propensity to develop bacterial resistance, silver can also be used to reduce the spread of methicillinresistant strains [1,2,19,21].…”

Section: Introductionmentioning

confidence: 99%

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Reactive combinatorial synthesis and characterization of a gradient Ag–Ti oxide thin film with antibacterial properties

et al. 2015

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Growing demand for orthopedic and dental implants has spurred researchers to develop multifunctional coatings, combining tissue integration with antibacterial features. A possible strategy to endow titanium (Ti) with antibacterial properties is by incorporating silver (Ag), but designing a structure with adequate Ag + release while maintaining biocompatibility has been shown difficult. To further explore the composition-structure-property relationships between Ag and Ti, and its effects against bacteria, this study utilized a combinatorial approach to manufacture and test a single sample containing a binary Ag-Ti oxide gradient. The sample, sputter deposited in a reactive (O 2 ) environment using a custom built combinatorial physical vapor deposition (PVD) system, was shown to be effective against Staphylococcus aureus with viability reductions ranging from 17 to above 99 %, depending on amount of Ag + released from its different parts. The Ag content along the gradient ranged from 35 to 62 wt%, but it was found that structural properties such as varied porosity and degree of crystallinity, rather than amount of incorporated Ag governed the Ag + release and resulting antibacterial activity. The coating also demonstrated in vitro apatite forming abilities, where structural variety along the sample was shown to alter the hydrophilic behavior, with degree of hydroxyapatite (HA) deposition varying accordingly. By means of combinatorial synthesis, a single gradient sample was able to display intricate compositional and structural features affecting its biological response, which would otherwise require a series of coatings. The current findings suggest that future implant coatings incorporating Ag as an antibacterial agent could be structurally enhanced to better suit clinical requirements.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reactive combinatorial synthesis and characterization of a gradient Ag–Ti oxide thin film with antibacterial properties

et al. 2015

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“…[49][50][51] During the whole experiment, the soluble silver concentration remained in the ppb range. Silver concentrations as low as 0.1 ppb have been reported as having a bactericidal effect in water.…”

Section: Release Of Silver Ions From the Coatingmentioning

confidence: 98%

On the long term antibacterial features of silver-doped diamondlike carbon coatings deposited via a hybrid plasma process

et al. 2014

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Environmental surfaces are increasingly recognized as important sources of transmission of hospital-acquired infections. The use of antibacterial surface coatings may constitute an effective solution to reduce the spread of contamination in healthcare settings, provided that they exhibit sufficient stability and a long-term antibacterial effect. In this study, silver-incorporated diamondlike carbon films (Ag-DLC) were prepared in a continuous, single-step plasma process using a hybrid, inductively coupled plasma reactor combined with a very-low-frequency sputtering setup. The average Ag concentration in the films, ranging from 0 to 2.4 at. %, was controlled by varying the sputtering bias on the silver target. The authors found that the activity of Escherichia coli was reduced by 2.5 orders of magnitude, compared with the control surface, after a 4-h contact with a 2.4 at. % Ag-DLC coating. The coatings displayed slow release kinetics, with a total silver ion release in the sub-ppb range after 4 h in solution, as measured by graphite furnace-atomic absorption spectroscopy. This was confirmed by Kirby–Bauer diffusion tests, which showed limited diffusion of biocidal silver with a localized antibacterial effect. As a slow and continuous release is mandatory to ensure a lasting antibacterial effect, the newly developed Ag-DLC coatings appears as promising materials for environmental hospital surfaces.

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“…Each evaluated samples could release 20.82 and 127.75 µg of Ag + per disk and showed markedly enhanced killing of the MRSA inoculums with 98% and >99.75% respectively within 24 h of incubation, while their silver free counterpart sample allowed the bacteria to grow up to 1000-fold. The non-cumulative release of silver ions of 0.4 ppm, 0.26 ppm and 0.005 ppm for 1 h, 24 h and 7 days respectively after immersion in water, from nanometer scale Ag-TiO2 composite film was demonstrated by Yu et al [34] and they also reported that 0.4 ppm released silver from Ag-TiO2 composite film is sufficient to cause almost 100% killing of E. coli when exposed to UV for 1 h. Studies by Jamuna-Thevi et al [48], reported nanostuctured Ag + doped TiO2 coatings deposited by RF magnetron on stainless steel, with overall Ag + ion release measured between 0.45 and 122 ppb. They also noted that at least 95 ppb Ag + ion released in buffered saline was sufficient for 99.9% of reduction against S. aureus after 24 h of incubation.…”

Section: Quantitative Comparisionsmentioning

confidence: 93%

Antibiofilm Activity of Epoxy/Ag-TiO2 Polymer Nanocomposite Coatings against Staphylococcus Aureus and Escherichia Coli

Santhosh¹,

Natarajan²

2015

Coatings

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Dispersion of functional inorganic nano-fillers like TiO2 within polymer matrix is known to impart excellent photobactericidal activity to the composite. Epoxy resin systems with Ag + ion doped TiO2 can have combination of excellent biocidal characteristics of silver and the photocatalytic properties of TiO2. The inorganic antimicrobial incorporation into an epoxy polymeric matrix was achieved by sonicating laboratory-made nano-scale anatase TiO2 and Ag-TiO2 into the industrial grade epoxy resin. The resulting epoxy composite had ratios of 0.5-2.0 wt% of nano-filler content. The process of dispersion of Ag-TiO2 in the epoxy resin resulted in concomitant in situ synthesis of silver nanoparticles due to photoreduction of Ag + ion. The composite materials were characterized by DSC and SEM. The glass transition temperature (Tg) increased with the incorporation of the nanofillers over the neat polymer. The materials synthesized were coated on glass petri dish. Anti-biofilm property of coated material due to combined release of biocide, and photocatalytic activity under static conditions in petri dish was evaluated against Staphylococcus aureus ATCC6538 and Escherichia coli K-12 under UV irradiation using a crystal violet binding assay. Prepared composite showed significant inhibition of biofilm development in both the organisms. Our studies indicate that the effective dispersion and optimal release of biocidal agents was responsible for anti-biofilm activity of the surface. The reported thermoset coating materials can be used as bactericidal surfaces either in industrial or healthcare settings to reduce the microbial loads. OPEN ACCESSCoatings 2015, 5 96

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Quantification of silver ion release, in vitro cytotoxicity and antibacterial properties of nanostuctured Ag doped TiO2 coatings on stainless steel deposited by RF magnetron sputtering

Cited by 154 publications

References 22 publications

Reactive combinatorial synthesis and characterization of a gradient Ag–Ti oxide thin film with antibacterial properties

Reactive combinatorial synthesis and characterization of a gradient Ag–Ti oxide thin film with antibacterial properties

On the long term antibacterial features of silver-doped diamondlike carbon coatings deposited via a hybrid plasma process

Antibiofilm Activity of Epoxy/Ag-TiO2 Polymer Nanocomposite Coatings against Staphylococcus Aureus and Escherichia Coli

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