Quantification of total polyphenols and flavonoids, antioxidant activity, and Sinensetin and Imperatorin contents of Imperata cylindrica root ethanol extract

Abstract

Introduction: Imperata cylindrica, commonly known as cogon grass, is currently widely distributed and used as a medicinal plant. The major compounds that have been isolated and identified are polyphenols and flavonoids, which have many biological activities such as antioxidant, and anticancer. Polyphenols and flavonoids are mostly found in the roots and leaves. This study aimed to perform phytochemical screening on I. cylindrica root ethanol extract from Sragen, Central Java, Indonesia and determine the total polyphenol, flavonoid, antioxidant activity and quantify Sinensetin and Imperatorin contents of the extract. Method: Quantification of all parameters were measured using visible spectrophotometric methods. Total polyphenol, total flavonoid contents, as well as antioxidant activity were measured using Folin-Ciocalteu reagent, aluminum chloride reagent, and 1,1-diphenyl-2-picrylhydrazyl, respectively, and quantification of Sinensetin and Imperatorin were measured using High Performance Liquid Chromatography. Results: I cylindrica root ethanol extract had a total polyphenol content of 1.109% gallic acid equivalent, total flavonoid content of 0.1% quercetin equivalent, and antioxidant activity IC 50 824.30 µg/ml. Sinensetin and Imperatorin were also identified in Fractions 1 to 11 with concentrations of 0.0157 and 0.0178 mg/kg extract, respectively. Conclusion: I. cylindrica root ethanol extract from Sragen had active phytochemical compounds of polyphenols, flavonoids, and antioxidants as well as Sinensetin and Imperatorin.

The effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro

“…18 Previously, sinensetin and imperatorin were found in similar concentrations in a root ethanol extract of Imperata cylindrica. 19 Researchers have found that imperatorin combined with other drugs works better in vitro. The combination of quercetin (50-100 μM) and imperatorin (50-100 μM) was more effective in HeLa cells and Hep-2 cells than either drug alone.…”

Prebiotic Effect of Cogon Grass Root Extract on Stimulating Lactic Acid Bacteria Growth in Mice Intestine

“…12,[18][19][20] The root of Cogon grass (Imperata cylindrica (L.)), is a common traditional medicine in Indonesia, 21 has been proven to have antioxidant and anti-inflammatory properties due to its polyphenols contents. [22][23][24][25] Previous study found pretreatment of mice with oral cogon grass root extract (CGRE) for two weeks, significantly reduced serum cholesterol levels after acute olive oil loading. 25 There is evidence polysaccharide from root of I. cylindrica can reduce cholesterol level, 26 and we want to know if it also has impact on LAB.…”

“…[22][23][24][25] Previous study found pretreatment of mice with oral cogon grass root extract (CGRE) for two weeks, significantly reduced serum cholesterol levels after acute olive oil loading. 25 There is evidence polysaccharide from root of I. cylindrica can reduce cholesterol level, 26 and we want to know if it also has impact on LAB. Thefore, the objective of this study is to explore whether the GCRE has prebiotic effects to stimulate LAB growth in mice intestine.…”