Quantifying chelonid herpesvirus 5 in symptomatic and asymptomatic rehabilitating green sea turtles

Page-Karjian, Annie; Norton, Terry M.; Ritchie, Branson W.; Brown, Corrie C.; Mancia, Carmen; Jackwood, Mark W.; Gottdenker, Nicole

doi:10.3354/esr00687

Cited by 38 publications

(62 citation statements)

References 47 publications

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“…Although ChHV5 has been associated with fibropapillomatosis and ChHV5 DNA is readily detectable within tumors 5,31 , there was an almost complete lack of viral transcripts within the RNA-seq samples, with only four ChHV5 transcripts being detected, three of which were related transcript variants (Fig. 2c, Supplementary Table 1).…”

Section: Resultsmentioning

confidence: 99%

Sea turtle fibropapilloma tumors share genomic drivers and therapeutic vulnerabilities with human cancers

et al. 2018

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Wildlife populations are under intense anthropogenic pressures, with the geographic range of many species shrinking, dramatic reductions in population numbers and undisturbed habitats, and biodiversity loss. It is postulated that we are in the midst of a sixth (Anthropocene) mass extinction event, the first to be induced by human activity. Further, threatening vulnerable species is the increased rate of emerging diseases, another consequence of anthropogenic activities. Innovative approaches are required to help maintain healthy populations until the chronic underlying causes of these issues can be addressed. Fibropapillomatosis in sea turtles is one such wildlife disease. Here, we applied precision-medicine-based approaches to profile fibropapillomatosis tumors to better understand their biology, identify novel therapeutics, and gain insights into viral and environmental triggers for fibropapillomatosis. We show that fibropapillomatosis tumors share genetic vulnerabilities with human cancer types, revealing that they are amenable to treatment with human anti-cancer therapeutics.

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Section: Resultsmentioning

confidence: 99%

Sea turtle fibropapilloma tumors share genomic drivers and therapeutic vulnerabilities with human cancers

et al. 2018

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“…Although the causative agent of FP is yet to be confirmed, studies have consistently reported a link between FP tumours and the presence of a herpesvirus [7][8][9]. As this virus could not be cultured in vitro until recently [10], there has been an increase in studies utilizing molecular methods to better understand this herpesvirus [11][12][13][14][15][16][17][18][19][20][21][22][23]. These studies have added to the body of evidence linking a turtle-specific herpesvirus, known as chelonid alphaherpesvirus 5 (ChHV5), and FP.…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia

et al. 2020

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Fibropapillomatosis (FP) is a marine turtle disease recognised by benign tumours on the skin, eyes, shell, oral cavity and/or viscera. Despite being a globally distributed disease that affects an endangered species, research on FP and its likely causative agent chelonid alphaherpesvirus 5 (ChHV5) in Australia is limited. Here we present improved molecular assays developed for detection of ChHV5, in combination with a robust molecular and phylogenetic analysis of ChHV5 variants. This approach utilised a multi-gene assay to detect ChHV5 in all FP tumors sampled from 62 marine turtles found at six foraging grounds along the Great Barrier Reef. Six distinct variants of ChHV5 were identified and the distribution of these variants was associated with host foraging ground. Conversely, no association between host genetic origin and ChHV5 viral variant was found. Together this evidence supports the hypothesis that marine turtles undergo horizontal transmission of ChHV5 at foraging grounds and are unlikely to be contracting the disease at rookeries, either during mating or vertically from parent to offspring.

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“…The virus associated with FP, chelonid herpesvirus 5 (ChHV5), is believed to have co-evolved with sea turtles for approximately 1 million yr (Herbst et al 2004), and has genetic variants between populations (Morrison et al 2018). ChHV5 is found in FP tumors (Herbst 1994, Herbst & Klein 1995, Quackenbush et al 1998, Greenblatt et al 2005, Page-Karjian et al 2015 and is likely the cause of this disease, though the etiology is not completely understood. There is evidence that turtles acquire the virus after they recruit to a shallow foraging habitat (Ene et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Fibropapillomatosis prevalence and distribution in green turtles Chelonia mydas in Texas (USA)

Shaver¹,

Walker²,

Backof³

2019

Dis. Aquat. Org.

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Fibropapillomatosis (FP) is a neoplastic disease that can result in debilitating tumors in sea turtles. Initially identified in Florida, USA in 1937, it has since been detected in green turtle Chelonia mydas populations globally. FP was first identified and confirmed in Texas, USA in 2010. No FP tumors were documented in Texas prior to that year, though many green turtles were encountered and examined using standardized procedures since 1980. The present study was undertaken to identify temporal and spatial trends of FP prevalence in Texas since 2010. From 2010 through 2018, 1919 stranded or incidentally captured green turtles were documented with FP in Texas. FP prevalence was significantly correlated with year, hypothermic stunning, geographic region, and turtle size, as determined by logistic regression. FP was documented in < 4.0% of the green turtles examined in Texas from 2010 to 2015, increasing to 21.6% in 2016, 27.3% in 2017, and 35.2% in 2018. More than twice as many hypothermic stunned green sea turtles had FP tumors as compared to those that were not hypothermic stunned. In Texas, FP was most prevalent in south Texas, particularly in the Laguna Madre, and associated channels. FP was more prevalent in turtles with straight carapace lengths 40.0−69.9 cm. The impact of this disease on green turtle population recovery in Texas is not yet apparent.

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Quantifying chelonid herpesvirus 5 in symptomatic and asymptomatic rehabilitating green sea turtles

Cited by 38 publications

References 47 publications

Sea turtle fibropapilloma tumors share genomic drivers and therapeutic vulnerabilities with human cancers

Sea turtle fibropapilloma tumors share genomic drivers and therapeutic vulnerabilities with human cancers

Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia

Fibropapillomatosis prevalence and distribution in green turtles Chelonia mydas in Texas (USA)

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