Quantifying Embolism: Label‐Free Volumetric Mapping of Thrombus Structure and Kinesis in a Microfluidic System with Optical Holography

Abstract

Label-free imaging of a developing thrombus 2. Holographic QPM Imaging for real time volume quantification 3. Growth, contraction and stability of a thrombus formed on a microfluidic chip. 4. In vitro studies of embolization

Advanced Optical Imaging of Blood Thrombus

“…This is especially because light sheet microscopy uses very low density of light and causes almost no adverse phototoxic outcomes. The lightsheet component of the microscopy system will be able to capture individual calcium signals of platelets interacting and at the same time a QPM [6] can directly quantifies the overall growth of the thrombus under continuous fluid flows. The simultaneous acquisition of these parameters will allow investigators to study the interplay between microrheological and agonist-dependent mechanisms of heterogeneous platelet activation within a developing thrombus with un-parallel clarity and resolution not previously obtained.…”

Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro

“…1 C), but not both. The net stability of a thrombus under a parabolic velocity flow profile (4)(5)(6)(7)(8), as shown in Fig. 1 D, is dependent on a complex set of biophysical parameters.…”

“…To see this figure in color, go online. (7,17), diffraction phase microscopy (18) optical diffraction tomography (19), or near-field light scattering, i.e., reflectance interference contrast microscopy (RICM) (20). Furthermore, both interferometry-and scattering-based techniques are widefield (e.g., transillumination or epi-illumination) and collect the phase shift or scatters from the whole sample (21), which makes it difficult to selectively image only adhesive platelets.…”

Coherent Optical Scattering and Interferometry (COSI) for label-free multiparameter quantitative imaging of intra-thrombus stabilityin vitro

“…1C), but not both. The net stability of a thrombus under a parabolic velocity flow profile (4)(5)(6)(7)(8), as shown in Fig.1D, is dependent on a complex set of biophysical parameters. The ability of a thrombus to remain stable can be defined by two key physical interactions.…”

“…However, current platelet label-free imaging employs either classical interferometry (15) i.e. quantitative phase microscopy (QPM) (7,16), diffraction phase microscopy (17) optical diffraction tomography (18) or near field light scattering i.e. reflectance interference contrast microscopy (RICM) (19).…”