Quantifying Recording Quality in In Vivo Striatal Recordings

Friend, Danielle M.; Kemere, Caleb; Kravitz, Alexxai V.

doi:10.1002/0471142301.ns0628s70

Cited by 5 publications

(7 citation statements)

References 27 publications

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“…Single units were isolated by sorting spikes based on waveform shape in Spike2 and confirmed by both PCA and an interspike interval of Ͼ1 ms [average number of spikes per unit assignment ϭ 3,679.5 Ϯ 450.2; for all NCM units 0% of interstimulus intervals (ISIs) occurred in the 1-ms bin]. For each PCA we quantified the J3 statistic, which is expressed as a ratio of the variance within a PCA cluster to the variance between two identified clusters (see, e.g., Friend et al 2015). Our single units had J3 values ranging from 2.39 to 6.27 (mean Ϯ SE ϭ 4.08 Ϯ 0.64), reflecting excellent isolation in PC space (J3 values Ͼ 2 are considered to reflect well-isolated single units; Friend et al 2015;Pais-Vieira et al 2013;Wolff et al 2014).…”

Section: Subjectsmentioning

confidence: 99%

“…For each PCA we quantified the J3 statistic, which is expressed as a ratio of the variance within a PCA cluster to the variance between two identified clusters (see, e.g., Friend et al 2015). Our single units had J3 values ranging from 2.39 to 6.27 (mean Ϯ SE ϭ 4.08 Ϯ 0.64), reflecting excellent isolation in PC space (J3 values Ͼ 2 are considered to reflect well-isolated single units; Friend et al 2015;Pais-Vieira et al 2013;Wolff et al 2014). Spike waveform quarter width durations (SQW; the duration of the waveform at 25% of the highest absolute peak value) and peak-to-peak durations were computed from the averaged waveforms.…”

Section: Subjectsmentioning

confidence: 99%

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Norepinephrine enhances song responsiveness and encoding in the auditory forebrain of male zebra finches

Lee

Pawlisch

Macedo-Lima

et al. 2018

Journal of Neurophysiology

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Norepinephrine (NE) can dynamically modulate excitability and functional connectivity of neural circuits in response to changes in external and internal states. Regulation by NE has been demonstrated extensively in mammalian sensory cortices, but whether NE-dependent modulation in sensory cortex alters response properties in downstream sensorimotor regions is less clear. Here we examine this question in male zebra finches, a songbird species with complex vocalizations and a well-defined neural network for auditory processing of those vocalizations. We test the hypothesis that NE modulates auditory processing and encoding, using paired extracellular electrophysiology recordings and pattern classifier analyses. We report that a NE infusion into the auditory cortical region NCM (caudomedial nidopallium; analogous to mammalian secondary auditory cortex) enhances the auditory responses, burst firing, and coding properties of single NCM neurons. Furthermore, we report that NE-dependent changes in NCM coding properties, but not auditory response strength, are transmitted downstream to the sensorimotor nucleus HVC. Finally, NE modulation in the NCM of males is qualitatively similar to that observed in females: in both sexes, NE increases auditory response strengths. However, we observed a sex difference in the mechanism of enhancement: whereas NE increases response strength in females by decreasing baseline firing rates, NE increases response strength in males by increasing auditory-evoked activity. Therefore, NE signaling exhibits a compensatory sex difference to achieve a similar, state-dependent enhancement in signal-to-noise ratio and coding accuracy in males and females. In summary, our results provide further evidence for adrenergic regulation of sensory processing and modulation of auditory/sensorimotor functional connectivity. NEW & NOTEWORTHY This study documents that the catecholamine norepinephrine (also known as noradrenaline) acts in the auditory cortex to shape local processing of complex sound stimuli. Moreover, it also enhances the coding accuracy of neurons in the auditory cortex as well as in the downstream sensorimotor cortex. Finally, this study shows that while the sensory-enhancing effects of norepinephrine are similar in males and females, there are sex differences in the mode of action.

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Section: Subjectsmentioning

confidence: 99%

Section: Subjectsmentioning

confidence: 99%

Norepinephrine enhances song responsiveness and encoding in the auditory forebrain of male zebra finches

Lee

Pawlisch

Macedo-Lima

et al. 2018

Journal of Neurophysiology

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“…Units were isolated based on waveform parameters including peak amplitude and principal components. Clusters containing similar valid waveforms were manually defined, and the single units did not contain a refractory period <1 ms. MANOVA test ( p < 0.05) was used to validate the single-unit nature of spikes (Friend et al, 2015 ). Neuroexplorer (Plexon) was used for further analysis.…”

Section: Methodsmentioning

confidence: 99%

PtNPs/PEDOT:PSS-Modified Microelectrode Arrays Reveal Electrophysiological Activities of Different Neurons in Medial Amygdala of Mice Under Innate Fear

Fan

Song

et al. 2022

Front. Neurosci.

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The medial amygdala (MA) plays an important role in the innate fear circuit. However, the electrophysiological mechanism of MA for processing innate fear needs to be further explored. In this study, we fabricated microelectrode arrays (MEAs) with detecting sites arranged to match the location and shape of MA in mice and detected the electrophysiology in freely behaving mice under 2-methyl-2-thiazoline (2MT)-induced fear. The detection performance of MEA is improved by modifying metal nanoparticles and conductive polymers (PtNPs/PEDOT:PSS). After modification, the impedance magnitude and phase of electrodes were decreased to 27.0 ± 2.3 kΩ and −12.30 ± 0.52°, respectively, leading to a signal-to-noise ratio of 10. Its electrochemical stability and mechanical stability were also verified by cyclic voltammetry (CV) sweeping and ultrasonic vibration. MEAs were then implanted into the MA of mice, and the electrophysiology and behavioral characteristics were synchronously recorded and analyzed. The results showed that 2MT induced strong defensive behaviors in mice, accompanied by increases in the average spike firing rate and local field potential (LFP) power of MA neurons. According to principles commonly applied to cortical extracellular recordings, the recorded neurons are divided into two classes based on waveforms. Statistics showed that about 37% of type 1 neurons (putative GABAergic neurons) and 87% of type 2 neurons (putative glutamatergic neurons) were significantly activated under innate fear. At the same time, the firing rate of some activated neurons had a good linear correlation with the freezing rate.

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“…We retained distinct units that did not substantially overlap other clusters in principle components space (Figure 2a); none of these units had an interspike interval violation within the 1 ms bin. All units used in the final analysis had a J3 statistic > 2 (mean ± SE = 3.74 ± 0.23), also indicating well‐isolated single units (Friend et al., 2015). We measured peak to peak duration (ms; depolarization peak to peak of after‐hyperpolarization) for each unit.…”

Section: Methodsmentioning

confidence: 99%

Adult‐like neural representation of species‐specific songs in the auditory forebrain of zebra finch nestlings

Schroeder

Remage‐Healey

2021

Developmental Neurobiology

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Encoding of conspecific signals during development can reinforce species barriers as well as set the stage for learning and production of species‐typical vocalizations. In altricial songbirds, the development of the auditory system is not complete at hatching, so it is unknown the degree to which recently hatched young can process auditory signals like birdsong. We measured in vivo extracellular responses to song stimuli in a zebra finch (Taeniopygia guttata) secondary auditory forebrain region, the caudomedial nidopallium (NCM). We recorded from three age groups between 13 days post‐hatch and adult to identify possible shifts in stimulus encoding that occur before the opening of the sensitive period of song motor learning. We did not find differences in putative cell type composition, firing rate, response strength, and selectivity across ages. Across ages narrow‐spiking units had higher firing rates, response strength, accuracy, and trial‐by‐trial reliability along with lower selectivity than broad‐spiking units. In addition, we showed that stimulus‐specific adaptation, a characteristic of adult NCM, was also present in nestlings and fledglings. These results indicate that most features of secondary auditory processing are already adult‐like shortly after hatching. Furthermore, we showed that selectivity for species‐specific stimuli is similar across all ages, with the greatest fidelity in temporal coding in response to conspecific song and domesticated Bengalese finch song, and reduced fidelity in response to owl finch song, a more ecologically relevant heterospecific, and white noise. Our study provides the first evidence that the electrophysiological properties of higher‐order auditory neurons are already mature in nestling songbirds.

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Quantifying Recording Quality in In Vivo Striatal Recordings

Cited by 5 publications

References 27 publications

Norepinephrine enhances song responsiveness and encoding in the auditory forebrain of male zebra finches

Norepinephrine enhances song responsiveness and encoding in the auditory forebrain of male zebra finches

PtNPs/PEDOT:PSS-Modified Microelectrode Arrays Reveal Electrophysiological Activities of Different Neurons in Medial Amygdala of Mice Under Innate Fear

Adult‐like neural representation of species‐specific songs in the auditory forebrain of zebra finch nestlings

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