Quantifying Serum Derived Differential Expressed and Low Molecular Weight Protein in Breast Cancer Patients

Zafar, Ayesha; Jabbar, Maryum; Manzoor, Yasmeen; Gulzar, Huma; Hassan, Shahzad Gul; Nazir, Muniba Anum; Ain-Ul-Haq,; Mustafa, Ghazala; Sahar, Romana; Masood, Aqeel; Iqbal, Ahtesham; Hussain, Mulazim; Hasan, Murtaza

doi:10.2174/0929866527666200110155609

Cited by 16 publications

(8 citation statements)

References 84 publications

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“…Tumor markers are the biochemical microstructure released by tumor, cancerous or other body cells in response of metastatic effect of cancer [42]. They are biologically more significant in indicating the cancer formation and progression inside various body organs or tissues.…”

Section: Tumor Marker For Diagnosis Of Cancermentioning

confidence: 99%

Blueprinting in Coherence to Diagnostic Momentum: On Account of Cancer Detection in Bahawalpur Community

Manzoor¹,

Dilshad²,

Hassan³

et al. 2022

PJBB

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In the current era, cancer is a critical health problem. Purpose of the study was symptomatic analysis of cancer patients and medical evaluation of hematological and biochemical parameters of patients from different geographical areas of district Bahawalpur. A crosssectional study was done to find out the diagnostic timeliness in cancer patients. Out of 150 cancer patients, 53% patients were males and 47% females with age from 15 years to 75 years. It has been shown that 76% of patients represent major symptoms of cancer while 24% of patients were showing minor symptoms. Furthermore, cancer patients were disturbed metabolical, hematological, and biochemical profiling. There were 83% of patients with anemia and 17% patients have normal hemoglobin count in males and 85 % of total female patients have low hemoglobin level while rest of 15% have normal hemoglobin count. Illiteracy is positively influencing the factors responsible for causing cancer with 53%, whereas 23% less than primary level education and only 1% of patients had completed their graduation. There were 31% of the patients lies in upper lower class of society, 28% lower class, Lower middle class 19%, middle class 14%, upper middle class 7% and only 1 % patients belong to high social status. This study will help to diagnosis the early cancer detection via biochemical and metabolic profiling.

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Section: Tumor Marker For Diagnosis Of Cancermentioning

confidence: 99%

Blueprinting in Coherence to Diagnostic Momentum: On Account of Cancer Detection in Bahawalpur Community

Manzoor¹,

Dilshad²,

Hassan³

et al. 2022

PJBB

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“…The percentage of hepatocellular injuries’ amelioration at different dosages of nanosilymarin was in the order 1500 > 1000 > 500 μg/kg bw. The current investigation is most related to work previously reported by the researchers. , …”

Section: Resultsmentioning

confidence: 86%

Designing a Silymarin Nanopercolating System Using CME@ZIF-8: An Approach to Hepatic Injuries

Yu,

Saif,

Hasan

et al. 2023

ACS Omega

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It is commonly known that silymarin, a phytoconstituent obtained from the Silybum marianum plant, has hepatoprotective and antioxidative properties. However, its low oral bioavailability and poor water solubility negatively impact its therapeutic efficacy. The goal of the present study was to determine the efficiency of the Cordia myxa extract-based synthesized zeolitic imidazole metal–organic framework (CME@ZIF-8 MOF) for increasing silymarin’s bioavailability. A coprecipitation technique was used to synthesize the CME@ZIF-8 and polyethylene glycol-coated silymarin-loaded MOFs (PEG-Sily@CME@ZIF-8) and a complete factorial design was used to optimize them. The crystalline size of CME@ZIF-8 was 14.7 nm and the size of PEG-Sily@CME@ZIF-8 was 17.39 nm. The loading percentage of the silymarin drug in CME@ZIF-8 was 33.5%. The optimized formulations were then characterized by ultraviolet–visible (UV–vis) spectroscopy, X-ray diffraction, Fourier transform IR spectroscopy, surface morphology, gas chromatography–mass spectrometry, and drug release in an in vitro medium. Additionally, a rat model was used to investigate the optimized formulation’s in vivo hepatoprotective effectiveness. The synthesized silymarin-loaded CME@ZIF-8 MOFs were distinct particles with a porous, spongelike shape and a diameter of (size) nm. Furthermore, the designed silymarin-loaded PEG-Sily@CME@ZIF-8 MOF formulation exhibited considerable silymarin release from the synthesized formula in dissolution investigations. The in vivo evaluation studies demonstrated that the prepared PEG-Sily@CME@ZIF-8 MOFs effectively exhibited a hepatoprotective effect in comparison with free silymarin in a CCl4-based induced-hepatotoxicity rat model via ameliorating the normal antioxidant enzyme levels and restoring the cellular abnormalities produced by CCl4 toxication. In combination, biologically produced CME@ZIF-8 may promise to be a viable biologically based nanocarrier that can enhance the loading and release of silymarin medication, which has low solubility in water.

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“…It also processes the viral polyproteins, targets the host interferon, and NF-κB pathways, and generates a functional replicase complex. Natural products are the freely available medicinal flora for the treatment of various diseases [34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Pharmacotherapeutic Potential of Natural Products to Target the SARS-CoV-2 PLpro Using Molecular Screening and Simulation Approaches

Sayaf

Ahmad

Aslam

et al. 2023

Appl Biochem Biotechnol

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Because of the essential role of PLpro in the regulation of replication and dysregulation of the host immune sensing, it is considered a therapeutic target for novel drug development.To reduce the risk of immune evasion and vaccine effectiveness, small molecular therapeutics are the best complementary approach. Hence, we used a structure-based drug-designing approach to identify potential small molecular inhibitors for PLpro of SARS-CoV-2. Initial scoring and re-scoring of the best hits revealed that three compounds NPC320891 (2,2-Dihydroxyindene-1,3-Dione), NPC474594 (Isonarciclasine), and NPC474595 (7-Deoxyisonarciclasine) exhibit higher docking scores than the control GRL0617. Investigation of the binding modes revealed that alongside the essential contacts, i.e., Asp164, Glu167, Tyr264, and Gln269, these molecules also target Lys157 and Tyr268 residues in the active site. Moreover, molecular simulation demonstrated that the reported top hits also possess stable dynamics and structural packing. Furthermore, the residues' flexibility revealed that all the complexes demonstrated higher flexibility in the regions 120-140, 160-180, and 205-215. The 120-140 and 160-180 lie in the finger region of PLpro, which may open/close during the simulation to cover the active site and push the ligand inside. In addition, the total binding free energy was reported to be − 32.65 ± 0.17 kcal/mol for the GRL0617-PLpro, for the NPC320891-PLpro complex, the TBE was − 35.58 ± 0.14 kcal/ mol, for the NPC474594-PLpro, the TBE was − 43.72 ± 0.22 kcal/mol, while for NPC474595-PLpro complex, the TBE was calculated to be − 41.61 ± 0.20 kcal/mol, respectively. Clustering of the protein's motion and FEL further revealed that in NPC474594 and NPC474595 complexes, the drug was seen to have moved inside the binding cavity along with the loop in the palm region harboring the catalytic triad, thus justifying the higher binding of these two molecules particularly. In conclusion, the overall results reflect favorable binding of the identified hits strongly than the control drug, thus demanding in vitro and in vivo validation for clinical purposes.

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Quantifying Serum Derived Differential Expressed and Low Molecular Weight Protein in Breast Cancer Patients

Cited by 16 publications

References 84 publications

Blueprinting in Coherence to Diagnostic Momentum: On Account of Cancer Detection in Bahawalpur Community

Blueprinting in Coherence to Diagnostic Momentum: On Account of Cancer Detection in Bahawalpur Community

Designing a Silymarin Nanopercolating System Using CME@ZIF-8: An Approach to Hepatic Injuries

Pharmacotherapeutic Potential of Natural Products to Target the SARS-CoV-2 PLpro Using Molecular Screening and Simulation Approaches

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