Quantifying the Metabolites of the Methylerythritol 4-Phosphate (MEP) Pathway in Plants and Bacteria by Liquid Chromatography–Triple Quadrupole Mass Spectrometry

González-Cabanelas, Diego; Hammerbacher, Almuth; Raguschke, Bettina; Gershenzon, Jonathan; Wright, Louwrance P.

doi:10.1016/bs.mie.2016.02.025

Cited by 23 publications

(17 citation statements)

References 67 publications

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“…Isoprenoid metabolites were analysed on an Agilent 1260 Infinity HPLC system (Agilent Technologies, Germany) connected to an API 5000 triple quadrupole mass spectrometer (Applied Biosystems, Germany). DXP, MEcPP, and IPP/DMAPP (eluting as a single peak) were separated on an XBridge Amide column (3.5 μm, 150 × 2.1 mm, Waters, Germany) with an HILIC guard column containing the same sorbent (3.5 μm, 10 × 2.1 mm) and an SSI™ high pressure precolumn filter (Sigma) as described in González‐Cabanelas et al (). The isoprenoid metabolites were quantified using external standard curves containing added internal standards (ISTD) consisting of [ 13 C]DXP, MEcPP, IPP, and DMAPP as described in González‐Cabanelas et al ().…”

Section: Methodsmentioning

confidence: 99%

“…Isoprenoid metabolites including 1-deoxy-D-xylulose 5-phosphate (DXP), MEcPP, IPP, and DMAPP were extracted as described by González-Cabanelas, Hammerbacher, Raguschke, Gershenzon, and Wright (2016). Briefly, approximately 5 mg of lyophilized plant material was extracted twice with 250 μl 50% acetonitrile containing 10 mM of ammonium acetate (pH 9.0).…”

Section: Analysis Of Mep Pathway Metabolitesmentioning

confidence: 99%

“…Sigma) as described inGonzález-Cabanelas et al (2016). The isoprenoid metabolites were quantified using external standard curves containing added internal standards (ISTD) consisting of [ 13 C]DXP, MEcPP, IPP, and DMAPP as described inGonzález-Cabanelas et al (2016).…”

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confidence: 99%

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The plastidial metabolite 2‐C‐methyl‐D‐erythritol‐2,4‐cyclodiphosphate modulates defence responses against aphids

Onkokesung

Reichelt

Wright

et al. 2019

Plant Cell & Environment

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Feeding by insect herbivores such as caterpillars and aphids induces plant resistance mechanisms that are mediated by the phytohormones jasmonic acid (JA) and salicylic acid (SA). These phytohormonal pathways often crosstalk. Besides phytohormones, methyl‐D‐erythriol‐2,4‐cyclodiphosphate (MEcPP), the penultimate metabolite in the methyl‐D‐erythritol‐4‐phosphate pathway, has been speculated to regulate transcription of nuclear genes in response to biotic stressors such as aphids. Here, we show that MEcPP uniquely enhances the SA pathway without attenuating the JA pathway. Arabidopsis mutant plants that accumulate high levels of MEcPP (hds3) are highly resistant to the cabbage aphid (Brevicoryne brassicae), whereas resistance to the large cabbage white caterpillar (Pieris brassicae) remains unaltered. Thus, MEcPP is a distinct signalling molecule that acts beyond phytohormonal crosstalk to induce resistance against the cabbage aphid in Arabidopsis. We dissect the molecular mechanisms of MEcPP mediating plant resistance against the aphid B. brassicae. This shows that MEcPP induces the expression of genes encoding enzymes involved in the biosynthesis of several primary and secondary metabolic pathways contributing to enhanced resistance against this aphid species. A unique ability to regulate multifaceted molecular mechanisms makes MEcPP an attractive target for metabolic engineering in Brassica crop plants to increase resistance to cabbage aphids.

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Section: Methodsmentioning

confidence: 99%

Section: Analysis Of Mep Pathway Metabolitesmentioning

confidence: 99%

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The plastidial metabolite 2‐C‐methyl‐D‐erythritol‐2,4‐cyclodiphosphate modulates defence responses against aphids

Onkokesung

Reichelt

Wright

et al. 2019

Plant Cell & Environment

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“…The MEP pathway metabolites were analyzed using the MRM conditions described by Wright et al (2014) . The metabolite concentrations were calculated by using external standard curves, and were normalized to the [ 13 C]-labeled internal standards of each intermediate ( González-Cabanelas et al, 2016 ) added to the extract after the first extraction step.…”

Section: Methodsmentioning

confidence: 99%

Rust Infection of Black Poplar Trees Reduces Photosynthesis but Does Not Affect Isoprene Biosynthesis or Emission

et al. 2018

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Poplar (Populus spp.) trees are widely distributed and play an important role in ecological communities and in forestry. Moreover, by releasing high amounts of isoprene, these trees impact global atmospheric chemistry. One of the most devastating diseases for poplar is leaf rust, caused by fungi of the genus Melampsora. Despite the wide distribution of these biotrophic pathogens, very little is known about their effects on isoprene biosynthesis and emission. We therefore infected black poplar (P. nigra) trees with the rust fungus M. larici-populina and monitored isoprene emission and other physiological parameters over the course of infection to determine the underlying mechanisms. We found an immediate and persistent decrease in photosynthesis during infection, presumably caused by decreased stomatal conductance mediated by increased ABA levels. At the same time, isoprene emission remained stable during the time course of infection, consistent with the stability of its biosynthesis. There was no detectable change in the levels of intermediates or gene transcripts of the methylerythritol 4-phosphate (MEP) pathway in infected compared to control leaves. Rust infection thus does not affect isoprene emission, but may still influence the atmosphere via decreased fixation of CO2.

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“…The diverse chemical structures of the SMs determine their functions to the medicine and stress adaptation. Three major categories are (i) terpenes derived from mevalonic acid pathway [25] and methyl erythritol phosphate pathway [26], (ii) phenolics derived from malonic acid pathway or a few case shikimic acid pathway [27], (iii) N (Nitrogen) and S (Sulfur) containing compounds via tricarboxylic acid cycle or sometimes via shikimic acid pathway. Among the pathways of SMs accumulation, shikimic acid, mevalonic acid, phenylpropanoic acid and methyl erythritol phosphate pathways are highly regulated under stress stimuli.…”

Section: Generation and Diversity Of Secondary Metabolites In Plantsmentioning

confidence: 99%

Role of Secondary Metabolites to Attenuate Stress Damages in Plants

Akhi¹,

Haque²,

Biswas³

2021

Antioxidants - Benefits, Sources, Mechanisms of Action

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Plants are constantly facing various threats posed by the biotic and abiotic stressors. To survive in these challenged environment, plants evolve a variety of defense mechanism. Among the various phytochemicals, secondary metabolites (SMs) accumulate higher amount under stressful conditions and initiate signaling functions to up-regulation of defense responsive genes. SMs ensures the survival, persistence and competitiveness of the plant against the threat generated under stressful conditions. Therefore, the signaling functions of SMs to protect the plant from biotic and abiotic stressors are getting importance in the recent times. In this chapter the contribution of SMs to protect the plant from specific environmental stresses has been discussed.

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Quantifying the Metabolites of the Methylerythritol 4-Phosphate (MEP) Pathway in Plants and Bacteria by Liquid Chromatography–Triple Quadrupole Mass Spectrometry

Cited by 23 publications

References 67 publications

The plastidial metabolite 2‐C‐methyl‐D‐erythritol‐2,4‐cyclodiphosphate modulates defence responses against aphids

The plastidial metabolite 2‐C‐methyl‐D‐erythritol‐2,4‐cyclodiphosphate modulates defence responses against aphids

Rust Infection of Black Poplar Trees Reduces Photosynthesis but Does Not Affect Isoprene Biosynthesis or Emission

Role of Secondary Metabolites to Attenuate Stress Damages in Plants

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