Quantisation of Oxidation of Medium-Chain Triglycerides in Preterm Infants1

Sulkers, Esther; Lafeber, Harry N; Sauer, Pieter J. J.

doi:10.1203/00006450-198910000-00003

Cited by 37 publications

(14 citation statements)

References 28 publications

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“…Although the oxidation rate of MCFA is much higher than that of LCFA (28), the study by Sulkers et al (29) shows that about half of the ingested MCT follows pathways other than oxidation. Among these, urinary loss of dicarboxylic acids, storage in adipose tissue, and conversion to LCFA has been described (11,27,30).…”

Section: Discussionmentioning

confidence: 96%

“…Furthermore, during MCT feeding the oxidation capacity for MCFA by the liver might be exceeded, resulting in incomplete hepatic clearance of dietary MCFA. The production of 13 CO 2 after a constant infusion of 13 C-labeled octanoate to preterm infants reached a plateau within 1-3 h, thus indicating that the amount of MCT that can be oxidized by the liver is limited (29). Hence, MCFA not metabolized by the liver may be incorporated into VLDL-triacylglycerols by the liver, or they may remain in blood as nonesterified fatty acids.…”

Section: Discussionmentioning

confidence: 99%

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Plasma fatty acids and [13C]linoleic acid metabolism in preterm infants fed a formula with medium-chain triglycerides

Rodríguez

Funke

Fink

et al. 2003

Journal of Lipid Research

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Most preterm infant formulas contain mediumchain triacylglycerols (MCT), but the effects of MCT on polyunsaturated fatty acid status and metabolism are controversial. Thus, we studied the effects of MCT on linoleic acid metabolism using stable isotopes. Enterally fed preterm infants were randomized to receive for 7 days 40% of fat as MCT (n ‫؍‬ 10) or a formula without MCT (n ‫؍‬ 9). At study day 5, infants received orally 2 mg/kg body weight of 13 C-labeled linoleic acid. Fatty acids in plasma lipid classes and 13 C enrichment of phospholipid fatty acids were measured and tracer oxidation was monitored. Compared with the control group, the MCT group showed lower breath 13 CO 2 and higher plasma triacylglycerol contents of octanoic acid, of decanoic acid, and of total long-chain polyunsaturated fatty acids (57.1 ؎ 4.4 mol/l vs. 37.9 ؎ 4.8 mol/l, P Ͻ 0.01). Concentrations of several polyunsaturated fatty acids in plasma phospholipids and non esterified fatty acids were higher in the MCT group. 13 C concentrations in phospholipid n-6 fatty acids indicated no difference in the relative conversion of linoleic to arachidonic acid. We conclude that oral MCT effectively reduce polyunsaturated fatty acid and long chain polyunsaturated fatty acid oxidation in preterm infants without compromising endogenous n-6 long chain polyunsaturated fatty acid synthesis.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Plasma fatty acids and [13C]linoleic acid metabolism in preterm infants fed a formula with medium-chain triglycerides

Rodríguez

Funke

Fink

et al. 2003

Journal of Lipid Research

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“…In particular, it has been used in humans to study the relationship between carbohydrate and fat metabolism during exercise (3,5,13,32,33). Additionally, octanoate is a prominent component of the medium-chain triglycerides (MCT) found in preterm infant formulas, accounting (with decanoate) for up to 85% of total fat oxidation (34). MCT oil is also used clinically in the treatment of inborn errors of metabolism (10,30).…”

mentioning

confidence: 99%

Octanoate oxidation measured by¹³C-NMR spectroscopy in rat skeletal muscle, heart, and liver

Walton¹,

Ebert²,

Haller³

2003

Journal of Applied Physiology

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Contribution of octanoate to the oxidative metabolism of the major sites of fatty acid oxidation (heart, liver, and resting and contracting skeletal muscle) was assessed in the intact rat with 13C-NMR spectroscopy. Under inhalation anesthesia, [2,4,6,8-13C4]octanoate was infused into the jugular vein and the sciatic nerve of one limb was stimulated for 1 h. Octanoate was a principal contributor to the acetyl-CoA pool in all tissues examined, with highest oxidation occurring in heart and soleus muscle followed by predominantly red portion of gastrocnemius muscle (RG), liver, and then white portion of gastrocnemius muscle (WG). Fractional contribution of 13C-labeled octanoate to the acetyl-CoA pool (Fc2) was 0.563 +/- 0.066 for heart and 0.367 +/- 0.054 for liver. Significant differences were observed between each of the muscle types during both rest and contraction. In muscle, Fc2 was highest in soleus (0.565 +/- 0.089 rested, 0.564 +/- 0.096 contracted), followed by RG (0.470 +/- 0.092 rested, 0.438 +/- 0.072 contracted), and lowest in WG (0.340 +/- 0.081 rested, 0.272 +/- 0.065 contracted). Our findings demonstrate that the fractional contribution of octanoate to oxidative metabolism correlates with oxidative capacity of the tissue and that octanoate metabolism increases in contracted muscle in proportion to the overall increase in oxidative rate.

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“…In newborn infants fed orally, we studied both the rate of oxidation of octanoic acid (C8) and olein (C18). In the case of octanoate, almost 50% of the tracer was recovered in expired air, while a plateau in 13C02 in expired air was obtained in 1-2 h (12). Recovery of I3CO2 during continuous infusion of olein was only 10-20%, while after 6-12 h no real plateau was obtained (unpublished observations).…”

Section: Measurement Of Fatty Acid Oxidationmentioning

confidence: 88%

Substrate utilization during the first weeks of life

et al. 1994

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It is assumed that substrate utilization changes markedly around birth, from mainly glucose utilization before, to glucose/fat utilization after birth. We studied substrate oxidation and turnover in preterm infants on the first day and during the first weeks of life. We found that only part of the glucose that is infused on the first day of life is oxidized, while glucose is also converted into fat at the same time. Almost half of the energy expenditure is provided by fat oxidation on day 7 and 28 of life. Fat oxidation is dependent on the type of fat oxidized; the rate of oxidation of medium chain triglycerides (MCT) is higher than that of long chain fatty acids. MCT can replace glucose as an energy source. Proteins contribute only to a small extent (approximately 7%) to the energy expenditure at all ages.

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Quantisation of Oxidation of Medium-Chain Triglycerides in Preterm Infants1

Cited by 37 publications

References 28 publications

Plasma fatty acids and [13C]linoleic acid metabolism in preterm infants fed a formula with medium-chain triglycerides

Plasma fatty acids and [13C]linoleic acid metabolism in preterm infants fed a formula with medium-chain triglycerides

Octanoate oxidation measured by¹³C-NMR spectroscopy in rat skeletal muscle, heart, and liver

Substrate utilization during the first weeks of life

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Quantisation of Oxidation of Medium-Chain Triglycerides in Preterm Infants1

Cited by 37 publications

References 28 publications

Plasma fatty acids and [13C]linoleic acid metabolism in preterm infants fed a formula with medium-chain triglycerides

Plasma fatty acids and [13C]linoleic acid metabolism in preterm infants fed a formula with medium-chain triglycerides

Octanoate oxidation measured by13C-NMR spectroscopy in rat skeletal muscle, heart, and liver

Substrate utilization during the first weeks of life

Contact Info

Product

Resources

About

Octanoate oxidation measured by¹³C-NMR spectroscopy in rat skeletal muscle, heart, and liver