Quantitation in high-performance micro-thin-layer chromatography

Ripphahn, J.; Halpaap, H.

doi:10.1016/s0021-9673(00)99944-8

Cited by 62 publications

(9 citation statements)

References 17 publications

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“…Their data and those of Ripphahn and Halpaap [34] using a variety of solvents and LiChrosorb particles in the range of diameters 5-40 lm were shown to conform to the relationship…”

Section: Capillary Action Driven Flow and Calcsupporting

confidence: 62%

“…For the combination of most nonpolar organic solvents and bare silica phases, wetting angles close to 08 are normally obtained on the surface of bare silica phases, and the value of cos may be assumed to be 1 [33]. Specific permeability values were found to show little variation over the complete set of solvents tested, and the average values of the two groups of workers were found to be in excellent agreement: k 0 (7.9 € 1.2)610 -3 [33] and (8.0 € 0.7)610 -3 [34]. Specific permeabilities were calculated from the caLC results using the slopes in Fig.…”

Section: Capillary Action Driven Flow and Calcmentioning

confidence: 98%

“…As well as low instrumentation costs and simplicity, other attractive features of TLC include the ability to run separations quickly and in parallel. TLC is routinely used as an economical separation technique for many analytical tasks and provides realistic solutions in cases where very high separation efficiency is not really necessary [10].Since the 1970s [11,12], developments in porous particulate stationary phases using high purity silica supports with spherical regularity, small particle size and narrow size distribution have allowed chromatographic dynamics to be optimised and HPLC to achieve impressive separation performance [13]. By contrast, irregular shaped adsorbents are still widely used to create TLC plates, although HPTLC has proved the usefulness of smaller particulate phases (down to 5 lm) with a narrow size distribution [14].…”

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confidence: 99%

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Capillary action liquid chromatography

Zhang

Bergström

Goodall³

et al. 2009

J of Separation Science

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Capillary action LC (caLC) is introduced as a technique using capillary action as the driving force to perform LC in capillary columns packed with HPLC type microparticulate materials. A dry packing method with centrifugal force was developed to prepare capillary columns in parallel (10 columns per 3 min) to support their disposable use in caLC. Using a digital microscope for real-time imaging and recording separations of components in a dye mixture, caLC was found to have flow characteristics similar to TLC. Based on the investigation of microparticulate HPLC silica gels of different size (1.5-10 microm) and a typical TLC grade irregular medium, Merck 60G silica, the van Deemter curves suggested molecular diffusion as the major contribution to band broadening in caLC. With Waters Xbridge 2.6 microm silica, plate heights down to 8.8 microm were obtained, comparable to those achievable in HPLC. Assisted by an image-processing method, the visual caLC separation was converted to a classical chromatogram for further data analysis and such a facility confirmed the observation of highly efficient bands.

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“…Their data and those of Ripphahn and Halpaap [34] using a variety of solvents and LiChrosorb particles in the range of diameters 5-40 lm were shown to conform to the relationship…”

Section: Capillary Action Driven Flow and Calcsupporting

confidence: 62%

Section: Capillary Action Driven Flow and Calcmentioning

confidence: 98%

mentioning

confidence: 99%

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Capillary action liquid chromatography

Zhang

Bergström

Goodall³

et al. 2009

J of Separation Science

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“…Radial development of planar chromatograms can be performed as circular (Izmailov and Schraiber 1938;Ripphahn and Halpaap 1977;Botz et al 1990;Nyiredy 2003a) and anticircular (Kaiser 1978;Studer and Traitler 1986;Issaq 1980).…”

Section: Classification Of the Modes Of Chromatogram Developmentmentioning

confidence: 99%

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Tuzimski

2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Sample preparation, detection, identification, and quantitative determination of biomolecules are presented in this chapter. Advantages of planar chromatography and the basic principles (chambers, sample application, and chromatogram development) are also described. Rapid detection of biomolecules plays a strategical role in their investigation. Hyphenated techniques such as planar chromatography coupled to UV diode array detection and to mass spectrometry provide on-line extensive structural information on the metabolites prior to their isolation. In this chapter, the combination of thin-layer chromatography (TLC) with biomolecules specific detection by diode array scanning (DAD), mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared spectroscopy (TLC-FTIR) is discussed. In the last part of the chapter, the reader will gain useful information about a recent method of planar chromatography -multidimensional planar chromatography (MD-PC) and information on application of different modes of multidimensional planar chromatography and combination of this technique with diode array detection (MD-PC-DAD) and HPLC-DAD for separation, detection, and qualitative and quantitative determination of biomolecules. Planar chromatography as a pilot technique for HPLC is also described.Biomolecules are widespread throughout the world. It is an organic molecule produced by a living organism, including large polymeric molecules such as proteins, polysaccharides, and nucleic acids as well as small molecules such as a primary metabolites, secondary metabolites, and natural products. Many sample-preparation techniques are used in biomolecule analysis; the method selected depends on the

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“…Two approaches to increasing the efficiency of TLC have been taken. One approach, the use of smaller, more closely sized particles in the thin layer (2), represents the application of general chromatographic principles to TLC. The second, Programmed Multiple Development (PMD) (3,4) represents a novel exploitation of TLC phenomena.…”

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confidence: 99%