Quantitation of Adenovirus Genome During Acute Infection in Normal Children

Shike, Hiroko; Shimizu, Chisato; Kanegaye, John T.; Foley, Jennifer L.; Burns, Jane C.

doi:10.1097/01.inf.0000148882.97399.79

Cited by 35 publications

(23 citation statements)

References 25 publications

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“…Various assays have used WB, PBMCs, plasma, and serum (Echavarria et al, 2001;Flomenberg et al, 1997;Gu et al, 2003;Heim et al, 2003;Lankester et al, 2002) and ADV has been demonstrated in all of these compartments. Lymphocytes or PBMCs have been proposed as primary sites of viral latency (Horowitz, 2001) in asymptomatic patients, while others have provided anecdotal evidence that most virus is extracellular during active infection (Shike et al, 2005). The findings described in the present study are consistent with this notion, in that both WB and plasma demonstrated consistently higher viral loads than did PBMC.…”

Section: Discussionsupporting

confidence: 94%

Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells

Perlman¹,

Gibson²,

Pounds³

et al. 2007

Journal of Clinical Virology

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Section: Discussionsupporting

confidence: 94%

Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells

Perlman¹,

Gibson²,

Pounds³

et al. 2007

Journal of Clinical Virology

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“…A PCR-based test using broadly cross-hybridizing primers is highly sensitive and specific but remains a research tool [37]. Immunofluorescence-based tests suffer from poor sensitivity (~50%), although these tests are highly specific, and a positive test result for cells from the nasopharynx suggests current infection [38].…”

Section: Discussionmentioning

confidence: 99%

Gene Transcript Abundance Profiles Distinguish Kawasaki Disease from Adenovirus Infection

Popper¹,

Watson²,

Shimizu³

et al. 2009

J INFECT DIS

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Background Acute Kawasaki disease (KD) is difficult to distinguish from other illnesses that involve acute rash or fever, in part because the etiologic agent(s) and pathophysiology remain poorly characterized. As a result, diagnosis and critical therapies may be delayed. Methods We used DNA microarrays to identify possible diagnostic features of KD. We compared gene expression patterns in the blood of 23 children with acute KD and 18 age-matched febrile children with 3 illnesses that resemble KD. Results Genes associated with platelet and neutrophil activation were expressed at higher levels in patients with KD than in patients with acute adenovirus infections or systemic adverse drug reactions, but levels in patients with KD were not higher than those in patients with scarlet fever. Genes associated with B cell activation were also expressed at higher levels in patients with KD than in control subjects. A striking absence of interferon-stimulated gene expression in patients with KD was confirmed in an independent cohort of patients with KD. Using a set of 38 gene transcripts, we successfully predicted the diagnosis for 21 of 23 patients with KD and 7 of 8 patients with adenovirus infection. Conclusions These findings provide insight into the molecular features that distinguish KD from other febrile illnesses and support the feasibility of developing novel diagnostic reagents for KD based on the host response.

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“…Several real-time adenovirus home-brew PCRs have been developed, initially for bone marrow transplant (BMT) patients and recently for other patient populations (20,29,46,73,98). A commercial real-time PCR assay (adenovirus r-gene PCR) was recently developed and validated for the generic detection of all adenovirus serotypes.…”

Section: Molecular Methodsmentioning

confidence: 99%

Adenoviruses in Immunocompromised Hosts

2008

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SUMMARY The number of patients with acquired immunodeficiency has grown steadily as a result of both a larger number of patients receiving solid organ and hematopoietic stem cell transplants and their longer survival times. The use of newer, more potent immunosuppressive regimens has increased the frequency of severe adenovirus infections. Human adenoviruses are a large group of viruses, represented by at least 52 serotypes with various genotypes divided into genomic clusters, and these may cause a broad variety of clinical manifestations. The development of molecular methods has increased the sensitivity and rapidity of adenovirus infection diagnosis. The implementation of PCR assays has significantly contributed to the identification of patients with disseminated adenovirus disease. More recently, the development of real-time PCR assays has permitted virus quantification and patient follow-up. There is no treatment for adenovirus with demonstrated efficacy, although cidofovir is widely used. Sensitive diagnostic tests for adenovirus can contribute to the early diagnosis and successful treatment of life-threatening adenovirus infections, especially in complex immunocompromised patients. The development of improved adenovirus therapy still remains a challenge. Adenovirus genetic diversity should be considered for diagnosis, typing, and therapeutic interventions.

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Quantitation of Adenovirus Genome During Acute Infection in Normal Children

Abstract: Viral load assessment in clinical samples determined by quantitative PCR can be useful in the diagnosis of adenovirus infection in immunocompetent, febrile children.

Cited by 35 publications

References 25 publications

Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells

Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells

Gene Transcript Abundance Profiles Distinguish Kawasaki Disease from Adenovirus Infection

Adenoviruses in Immunocompromised Hosts

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