Quantitation of antibody secreting cells in high and low antibody responder inbred carp (Cyprinus carpioL.) strains

Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP)

“…Nevertheless, a clear trend for higher production of ASC in the secondary responses to these antigens was observed in the frequencies of ASC in the head kidney and spleen compared to the respective primary responses. Additionally, the peaks of the secondary responses (7 days postimmunisation) were observed much earlier than the primary ones (20 days post-immunisation) being in agreement with those found for carp reared at similar temperatures (Rijkers et al, 1980;Bandin et al, 1997) and where a higher magnitude was shown for the anamnestic response to SRBC and DNP compared with the respective primary responses.…”

“…Similar times for primary peak days have been reported for di#erent species when immunised at their physiological optimum temperatures (Davidson et al, 1992;Siwicki & Dunier, 1993;Aaltonen et al, 1994). Bandin et al (1997) reported a peak day at 17 days post-immunisation for the primary response of outbred and inbred carp to DNP and Aeromonas salmonicida reared at 23 C. A significantly higher number of ASC were observed in the response to DNP compared with the response to P. damselae ssp. piscicida, mainly in the secondary responses of head kidney and spleen.…”

“…However, the assay is very labour intensive and more animals are di$cult to handle in such a study. In fact, a lower number of fish (2-5 for each sampling day) has been used by others for a similar number of sample points (Secombes et al, 1991;Davidson et al, 1992Davidson et al, , 1997Siwicki & Dunier, 1993;Bandin et al, 1997). Additionally, the presence in some fish of non-specific ASC for the antigens used, also increased the variability.…”

“…In addition to the kinetics of antibody responses, knowledge of which compartments are involved in the immune response after intraperitoneal injection, a route that might be suitable for inducing mucosal as well as systemic responses, is important for better understanding and use of the mechanisms of protection. A solid-phase enzyme-linked immunosorbent assay (ELISPOT; Czerkinsky et al, 1983) has been reported as an advantageous alternative to the haemolytic plaque assay, to monitor organ-specific antibody responses in fish (Secombes et al, 1991;Waterstrat et al, 1991;Davidson et al, 1992;Aaltonen et al, 1994;Bandin et al, 1997). In the present study, primary and secondary systemic (spleen and head kidney) and mucosal (gut) antibody secreting cell (ASC) responses of juvenile sea bass to intraperitoneally administered bacterial antigens (V. anguillarum and P. damselae ssp.…”

In vitro assay to select rainbow trout with variable resistance/susceptibility to viral haemorrhagic septicaemia virus

“…Models for immune-related traits have been studied, such as carp with differential antibody production (Wiegertjes et al 1996, Bandin et al 1997 and trout with differential lysozyme activity (Røed et al 2002) or natural killer-like activity (Zimmerman et al 2004). Trout with opposite resistances have also been used to investigate the response to ABSTRACT: The merit of a candidate criterion of resistance to viral haemorrhagic septicaemia virus (VHSV) was tested with the view of producing experimental trout progeny with a predictable level of resistance.…”