Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy

Keppel, K.E.; Campbell, Karen; Zuckermann, Federico A.; Greeley, E.A.; Schaeffer, David J.; Husmann, Robert J.

doi:10.1016/j.vetimm.2008.02.008

Cited by 93 publications

(112 citation statements)

References 19 publications

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“…Allergen immunotherapy has been shown to increase IL‐10 in cats with asthma 43 and dogs with atopic dermatitis 56. IL‐10 only showed a short increase in the tracheal wash sample, possibly due to an insufficient IL‐10 response and a deficiency of Treg cells, similar to what is reported for human asthma 57.…”

Section: Discussionsupporting

confidence: 61%

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

Klier

Geis

Steuer

et al. 2017

Immunity Inflam & Disease

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IntroductionNew therapeutic strategies to modulate the immune response of human and equine allergic asthma are still under extensive investigation. Immunomodulating agents stimulating T‐regulatory cells offer new treatment options beyond conventional symptomatic treatment or specific immunotherapy for human and equine allergic airway diseases, with the goal of a homoeostatic T‐helper cell balance. The aim of this study was to evaluate the effects of a nebulized gelatin nanoparticle‐CpG formulation (CpG‐GNP) with and without specific allergens for the treatment of spontaneous allergic equine asthma as a model for human asthma.MethodsTwenty equine asthma‐affected horses were treated either with CpG‐GNP alone or CpG‐GNP with allergens. Two specific allergens were selected for each horse based on history and an in‐vitro test. Each horse received seven administrations of the respective nebulized composition and was examined before treatment, immediately after and 6 weeks after the treatment course.ResultsClinical parameters such as breathing rate, indirect interpleural measurement, arterial blood gases, amount of tracheal mucus and percentage of neutrophils and cytokines in tracheal washes and serum samples were evaluated. Treatment with CpG‐GNP alone as well as in combinations with relevant allergens resulted in clinical improvement of nasal discharge, breathing rate, amount of secretion and viscosity, neutrophil percentage and partial oxygen pressure directly after and 6 weeks after treatment. There were no significant differences between the two treatments in clinical parameters or local cytokine profiles in the tracheal wash fluid (IL‐10, IFN‐g, and IL‐17). IL‐4 concentrations decreased significantly in both groups.ConclusionNonspecific CpG‐GNP‐based immunotherapy shows potential as a treatment for equine and possibly also human allergic asthma.

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Section: Discussionsupporting

confidence: 61%

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

Klier

Geis

Steuer

et al. 2017

Immunity Inflam & Disease

View full text Add to dashboard Cite

show abstract

“…Specific activity of lymphocytes is assessed by identifying CD (cluster of differentiation) molecules present on the cell surface. T cells with CD3 molecules are responsible for the transmission of signals to the cell interior (5,9). This determines the recognition of antigens presented by dendritic cells (Langerhan's cells in the skin) by molecules of the major histocompatibility complex (MHC).…”

Section: Introductionmentioning

confidence: 99%

Expression of CD3, CD4, CD8, CD21, and MHC II lymphocyte antigens and serum IL-10 concentration in dogs with atopic dermatitis complicated by purulent dermatitis

Taszkun

2013

Bulletin of the Veterinary Institute in Pulawy

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Dog blood samples were analysed cytometrically and immunoenzymatically. In dogs with atopic dermatitis complicated by purulent dermatitis, the percentage of lymphocytes with CD8, CD21 antigens, as well as IL-10 concentration in serum were significantly higher than in healthy dogs. The results indicate that immunosuppression induced by the activity of an infectious agent plays an important role at this stage of the disease. This thesis was further confirmed by a high positive correlation between MHC II CD3, CD4, and CD21expression and a negative correlation between cells with MHC II and CD8 lymphocytes.

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“…Also Wassom and Grieve (1998) referring to the unpublished data of de Weck mentions the limited time of the presence of IgE antibodies in the serum. It should also be added that earlier specific immunotherapy probably influences the later lower level of specific IgE antibodies (Keppel and Campbell 2008). Thus, in the present experiment the dogs with the immunotherapy in their treatment history were not qualified to the test.…”

Section: Comparison Of Ige Test Results With Intradermal Skins Testsmentioning

confidence: 79%

Comparison of IgE test results with intradermal skin tests for dust mites and storage mites in atopic dogs

Popiel¹,

Cekiera²

2015

Polish Journal of Veterinary Sciences

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Atopic dermatitis is one of the most frequent allergic diseases in dogs. There are many methods of treating its symptoms but specific immunotherapy has recently gained high popularity. Before the application of specific immunotherapy, it is necessary to identify the allergens provoking the reaction of hypersensitivity in the selected animal. This raises a question about the method of allergen identification the medical practitioner decides to use in order to obtain the most credible result. The authors of the present study decided to compare the results of intradermal allergic tests and the results of an IgE screening test carried out using the Fc ε RIα receptor method. The aim of the study was to compare the results of both tests directed to dust and storage mites. The study proves that in case of the IgE screening tests (for a group of allergens), the sensitivity is quite high (85 to 90.69%) but the specificity of these tests is insufficient (25 to 50%). In case of antibodies for the selected mites the sensitivity and specificity was too low (65.1 to 89.4% for the sensitivity, with only 14.2 to 33.3% for the specificity). Only in case of D. petronyssinus the results were higher with the sensitivity calculated at 65.1% and the specificity at 80%. The IgE screening test carried out using the Fc ε RIα receptor method is reliable only in case of screening test for mites and the intradermal allergic test remains the gold standard for allergy testing.

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Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy

Cited by 93 publications

References 19 publications

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

Expression of CD3, CD4, CD8, CD21, and MHC II lymphocyte antigens and serum IL-10 concentration in dogs with atopic dermatitis complicated by purulent dermatitis

Comparison of IgE test results with intradermal skin tests for dust mites and storage mites in atopic dogs

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