Quantitation of disaturated phosphatidylcholine and phosphatidylglycerol in amniotic fluid by fluorescence diminution: methodology and clinical results.

Relationships between methoxyindole and kynurenine pathway metabolites in plasma and urine in children suffering from febrile and epileptic seizures

“…Peak areas calculated for samples of urine were converted into the quantity of each kynurenine metabolite via the corresponding standard curve. Peak areas were always calculated using the square of the peak height (Narbona et al, 1986) instead of the original formula (peak area ¼ peak height × horizontal width of one-half peak height; Freer et al, 1979). The relationship between the sample and standard concentration and the square of the peak height is linear and always results in correlation coefficients of above 0·99.…”

“…The chromatographic method is highly sensitive, even when there are marked concentration variations among the five metabolites studied. The TLC method was subject to quality control (Freer et al, 1979). Within-run (within-plate) analytical variation for the kynurenine standards of eight replicates at every concentration of each metabolite was determined, and the coefficients of variation thus obtained were between 6·5% and 8·5%.…”

Measurement of the Lecithin/Sphingomyelin Ratio and Phosphatidylglycerol in Amniotic Fluid: An Accurate Method for the Assessment of Fetal Lung Maturity

“…The present results on the diagnostic value of phosphatidylglycerol may be valid only when the phospholipids are measured by two-dimensional thin-layer chromatography. Since lipids present in the amniotic fluid (also other than phosphatidylserine and phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, lecithin and sphingomyelin) may interfere with phosphatidylglycerol, the specificity of other phosphatidylglycerol assays (Cunningham et al, 1978;Gotelli et al, 1978;Freer et al, 1979;Tsai and Marshall, 1979;Mitnick et a/, 1980;Touchstone et al, 1980) remains to be proven.…”