Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression

Sitnik, Roberta; Pinho, João Renato Rebello

doi:10.1590/s0074-02761998000300027

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…The majority of patients adhering to a prescribed ART regimen achieve sustainable viral suppression, as indicated by undetectable plasma viral load (VL) (<50 copies/mL) [2]. However, drug resistance (DR) has become an obstacle in maximizing the clinical benefit of ART, and thus routine genotypic HIV DR testing is recommended for all HIV-infected patients for HIV DR surveillance and/or clinical monitoring purposes.…”

Section: Introductionmentioning

confidence: 99%

A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

Gupta

Taylor

Patterson

et al. 2017

BioMed Research International

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The prevalence of drug resistance (DR) mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV), supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research. Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR) and reverse transcriptase (RT) regions of HIV-1 pol gene across many HIV-1 subtypes from LLV plasma samples. HIV-spiked plasma samples from the External Quality Assurance Program Oversight Laboratory (EQAPOL), covering various HIV-1 subtypes, as well as clinical specimens were used to optimize and validate the protocol. Our results demonstrate that this protocol has a broad HIV-1 subtype coverage and viral load span with high sensitivity and reproducibility. Moreover, the protocol is robust even when plasma sample volumes are limited, the HIV viral load is unknown, and/or the HIV subtype is undetermined. Thus, the protocol is applicable for the initial amplification of the HIV-1 PR and RT genes required for subsequent genotypic DR assays.

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Section: Introductionmentioning

confidence: 99%

A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

Gupta

Taylor

Patterson

et al. 2017

BioMed Research International

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An assessment of the reproducibility of reverse transcription digital PCR quantification of HIV-1

Falak

Macdonald

Busby

et al. 2022

Methods

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Mutation patterns, Cross Resistance and Virological Failure Among HIV type-1 Patients in Alexandria, Egypt

et al. 2022

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Aim: The main purpose of this cross-sectional study was to detect the prevalence of drug resistance mutations related to nonnucleoside/nucleoside reverse transcriptase inhibitors (NNRTIs/NRTIs) and protease inhibitors (PIs). Patients & methods: Patients (n = 45) with HIV type-1 were recruited, 30 of whom were treatment naive and 15 treatment experienced. A partial pol gene covering the protease/reverse transcriptase (PRRT) region was amplified and then sequenced by the Sanger method. Results & conclusion: The most common NNRTI/NRTI-related mutations were ‘V179I (24%) and K103N (14.3%)’ and ‘M41L and V75M’ (14.3% each). M36I and H69K were the most prevalent PI-related mutations (86% each). The results of the current study serve as an initial crucial step in defining the overall prevalence of HIV type-1 drug resistance in Egypt.

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Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression

Abstract: In this study, HIV-1 viral load quantitation determined by Nucleic Acid

Cited by 3 publications

References 14 publications

A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

An assessment of the reproducibility of reverse transcription digital PCR quantification of HIV-1

Mutation patterns, Cross Resistance and Virological Failure Among HIV type-1 Patients in Alexandria, Egypt

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