Quantitation of lipid hydroperoxides using enhanced chemiluminescence

Volkova, P. O.; Алексеев, А. В.; Dzhatdoeva, A. A.; Проскурнина, Е. В.; Vladimirov, Yu. A.

doi:10.3103/s0027131416010181

Cited by 7 publications

(7 citation statements)

References 8 publications

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“…Coumarin derivatives have similar properties. Such quinolizidine derivatives of coumarin as coumarin-314 (C-314), coumarin-334 (C-334), and coumarin-525 (C-525) act as chemiluminescence enhancers in lipid peroxidation reactions [ 16 , 60 , 61 , 62 , 70 ]. Because of the selective chemiluminescence enhancement caused by free radical reactions involving lipids, these substances are most suitable for studying lipid peroxidation processes.…”

Section: Search For Physical Enhancers Of the Chemiluminescence Accom...mentioning

confidence: 99%

“…However, despite its structure, C-525 is quite often used as a chemiluminescence activator; e.g., when detecting lipid hydroperoxides in the lipid substrate– Fe 2+ system [ 16 ]. Experiments in a similar system based on C-334 showed that the chemiluminescence of the system containing cytochrome c complexed with cardiolipin is due to the lipoperoxidase and quasi-lipoxygenase activity of this nanoparticle, but not to the activity of non-heme iron via the Fenton reaction [ 81 ].…”

Section: Search For Physical Enhancers Of the Chemiluminescence Accom...mentioning

confidence: 99%

“…It has been repeatedly reported that C-314, C-334, and C-525 are fluorogenic substrates that do not react with mixture components [ 16 , 60 , 61 , 62 , 70 ]. Although these data were obtained in a non-enzymatic lipid peroxidation system [ 62 ], they were automatically projected on systems where this process is triggered by peroxidase.…”

Section: Search For Physical Enhancers Of the Chemiluminescence Accom...mentioning

confidence: 99%

“…Representatives of the Yu.A. Vladimirov scientific school [ 6 , 14 , 15 , 16 ] call these substances physical activators of chemiluminescence, thus extending the term activator to both groups of chemiluminescent reagents. The authors of [ 2 ] use the term activator to designate physical chemiluminescence activators only.…”

Section: Introductionmentioning

confidence: 99%

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Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Romodin

2022

Acta Naturae

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The present review examines the use of chemiluminescence detection to evaluate the course of free radical reactions in biological model systems. The application of the method is analyzed by using luminescent additives that enhance the luminescence thanks to a tripletsinglet transfer of the electron excitation energy from radical reaction products and its emission in the form of light with a high quantum yield; these additives are called chemiluminescence enhancers or activators. Examples of these substances are provided; differences between the so-called chemical and physical enhancers are described; coumarin derivatives, as the most promising chemiluminescence enhancers for studying lipid peroxidation, are considered in detail. The main problems related to the use of coumarin derivatives are defined, and possible ways of solving these problems are presented. Intrinsic chemiluminescence and the mechanism of luminescence accompanying biomolecule peroxidation are discussed in the first part of the review.

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Section: Search For Physical Enhancers Of the Chemiluminescence Accom...mentioning

confidence: 99%

Section: Search For Physical Enhancers Of the Chemiluminescence Accom...mentioning

confidence: 99%

Section: Search For Physical Enhancers Of the Chemiluminescence Accom...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Romodin

2022

Acta Naturae

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“…Detection of intrinsic chemiluminescence is used in the study of various biological model systems [ 29 , 128 , 129 ]. In addition to lipid peroxidation, NO synthesis also causes tissue chemiluminescence, as shown by J.F.…”

Section: Chemiluminescence and Its Mechanismmentioning

confidence: 99%

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 1

Romodin

2021

Acta Naturae

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The present review, consisting of two parts, considers the application of the chemiluminescence detection method in evaluating free radical reactions in biological model systems. The first part presents a classification of experimental biological model systems. Evidence favoring the use of chemiluminescence detection in the study of free radical reactions, along with similar methods of registering electromagnetic radiation as electron paramagnetic resonance, spectrophotometry, detection of infrared radiation (IR spectrometry), and chemical methods for assessing the end products of free radical reactions, is shown. Chemiluminescence accompanying free radical reactions involving lipids has been the extensively studied reaction. These reactions are one of the key causes of cell death by either apoptosis (activation of the cytochrome c complex with cardiolipin) or ferroptosis (induced by free ferrous ions). The concept of chemiluminescence quantum yield is also discussed in this article. The second part, which is to be published in the next issue, analyzes the application of chemiluminescence detection using luminescent additives that are called activators, a.k.a. chemiluminescence enhancers, and enhance the emission through the tripletsinglet transfer of electron excitation energy from radical reaction products, followed by light emission with a high quantum yield.

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Dynamic Monitoring of the Oxidation Process of Phosphatidylcholine Using SERS Analysis

Xiang

Liao

et al. 2018

Anal. Chem.

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Phosphatidylcholine oxidation is closely related to many neurodegenerative diseases. In this paper, Raman spectroscopy was proposed to continuously monitor the oxidation of phosphatidylcholine and provide deep understanding of this biochemical process. To increase the detection sensitivity, surface-enhanced Raman spectroscopy (SERS) with a micro−nanosilver-complex substrate was prepared by electrodeposition. The prepared SERS substrate had an enhancement factor as high as 7.8 × 10 7 , ensuring detection sensitivity in the phosphatidylcholine-oxidation process. It was illustrated that the oxidation of phosphatidylcholine in an ethanol−water solution under the experimental conditions could be monitored and well described by second-order kinetics by continuously measuring and analyzing the SERS spectra of phosphatidylcholine-oxidation intermediates in 20 days. Meanwhile, the oxidation products were confirmed by mass spectrometry, and the oxidation process was in good concordance with mass-spectrometry detection. The use of SERS in following a biochemical process has advantages, including simple instrumentation, a low cost, a short detection time, and no sample pretreatment. Therefore, as a kind of vibration spectrum, SERS is preferable to traditional detection approaches such as MS, HPLC, and MRI for the dynamic monitoring and analysis of complex biochemical processes.

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Quantitation of lipid hydroperoxides using enhanced chemiluminescence

Cited by 7 publications

References 8 publications

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 1

Dynamic Monitoring of the Oxidation Process of Phosphatidylcholine Using SERS Analysis

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