Quantitation of the mRNA levels of Epo and EpoR in various tissues in the ovine fetus

David, R.B.; Lim, Gaik Bee; Koukoulas, Irene; Wintour, E. M.

doi:10.1016/s0303-7207(01)00718-3

Cited by 19 publications

(19 citation statements)

References 58 publications

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“…The partial ovine EpoR cDNA sequences, spanning from exons 2 to 8, has been published (David et al, 2002). The PCR primers designed to amplify ovine EpoR amplified the two distinct expected bands ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…2A). A 93-bp insert between exons 7 and 8 potentially results in expression of a longer EpoR protein as described earlier (David et al, 2002). We designed three different primer sets (EpoRI, EpoRII, and EpoRIII) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…EpoR mRNA splicing variants have been reported previously for human, mouse, rat, and sheep (Kuramochi et al, 1990;Nakamura et al, 1992;Yamaji et al, 1996;David et al, 2002). However, the role of different EpoR transcripts has not been well understood.…”

Section: Discussionmentioning

confidence: 98%

“…Human EpoR gene has been cloned from a placenta genomic library and characterized intensively (Maouche et al, 1991;Noguchi et al, 1991). Different EpoR isoforms derived by alternative splicing have been reported for the human, mouse, rat, and ovine EpoR genes (Kuramochi et al, 1990;Nakamura et al, 1992;Yamaji et al, 1996;David et al, 2002).…”

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confidence: 99%

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Evidence of Receptor-Mediated Elimination of Erythropoietin by Analysis of Erythropoietin Receptor mRNA Expression in Bone Marrow and Erythropoietin Clearance During Anemia

Nalbant

Saleh²,

Goldman³

et al. 2010

J Pharmacol Exp Ther

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Erythropoietin (Epo) is the primary hormone that stimulates erythroid proliferation and differentiation through its cell surface receptor (EpoR) on erythroid progenitor cells. Previous studies have suggested that the bone marrow plays an important role in Epo's elimination. The changes in the EpoR mRNA levels and Epo's clearance in the bone marrow of 11 newborn lambs were studied to elucidate the role of EpoR in Epo's clearance under anemic conditions. Epo mRNA levels were measured by realtime polymerase chain reaction, and relative expression of EpoR was calculated by using the comparative CT method. The glyceraldehyde-3-phosphate dehydrogenase housekeeping gene was chosen as a control gene for the calculations. All lambs showed significant increase in bone marrow EpoR mRNA levels after phlebotomy-induced anemia. Epo's clearance determined from simultaneous pharmacokinetic studies with 125 I-recombinant human Epo showed a significant increase after phlebotomy-induced anemia that was similar to the increase in EpoR. By day 28 after phlebotomy, EpoR mRNA levels and Epo clearance had returned toward baseline. These results indicate that the changes in Epo's clearance are not caused by body growth but result from significant changes in the pool of EpoR. A linear mixed-effect model was used to evaluate the quantitative relationship between EpoR and Epo's clearance. This analysis demonstrated a highly significant positive linear correlation between EpoR and Epo clearance. Together, these findings provide strong evidence that receptormediated Epo clearance is an important route for Epo's elimination.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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Evidence of Receptor-Mediated Elimination of Erythropoietin by Analysis of Erythropoietin Receptor mRNA Expression in Bone Marrow and Erythropoietin Clearance During Anemia

Nalbant

Saleh²,

Goldman³

et al. 2010

J Pharmacol Exp Ther

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“…EPO-R brain expression has been observed during development and adulthood in human and non-human primates and other mammals [10,[23][24][25][26]. In the developing mouse brain during mid gestation, EPO-R localizes in the neural tube in the neuroepithelium that contains proliferating neuroprecursors [8,23].…”

Section: Epo-r In Brainmentioning

confidence: 99%

Role of erythropoietin in the brain

Noguchi

Asavaritikrai

Teng

et al. 2007

Critical Reviews in Oncology/Hematology

210

159

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Multi-tissue erythropoietin receptor (EPO-R) expression provides for erythropoietin (EPO) activity beyond its known regulation of red blood cell production. This review highlights the role of EPO and EPO-R in brain development and neuroprotection. EPO-R brain expression includes neural progenitor cells (NPC), neurons, glial cells and endothelial cells. EPO is produced in brain in a hypoxia sensitive manner, stimulates NPC proliferation and differentiation, and neuron survival, and contributes to ischemic preconditioning. Mice lacking EPO or EPO-R exhibit increased neural cell apoptosis during development before embryonic death due to severe anemia. EPO administration provides neural protection in animal models of brain ischemia and trauma, reducing the extent of injury and damage. EPO stimulation of endothelial cells contributes to neuroprotection and is of particular importance since only low levels of EPO appear to cross the blood-brain barrier when administered at high dose intravenously. The therapeutic potential of EPO for brain ischemia/trauma and neurodegenerative diseases has shown promise in early clinical trial and awaits further validation.

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The Hematopoietic System

Car¹,

Seelig²

2022

Schalm's Veterinary Hematology

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Quantitation of the mRNA levels of Epo and EpoR in various tissues in the ovine fetus

Cited by 19 publications

References 58 publications

Evidence of Receptor-Mediated Elimination of Erythropoietin by Analysis of Erythropoietin Receptor mRNA Expression in Bone Marrow and Erythropoietin Clearance During Anemia

Evidence of Receptor-Mediated Elimination of Erythropoietin by Analysis of Erythropoietin Receptor mRNA Expression in Bone Marrow and Erythropoietin Clearance During Anemia

Role of erythropoietin in the brain

The Hematopoietic System

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