Quantitation of triacylglycerols in edible oils by off-line comprehensive two-dimensional liquid chromatography–atmospheric pressure chemical ionization mass spectrometry using a single column

Separation of Triacylglycerols from Edible Oil Using a Liquid Chromatography–Mass Spectrometry System with a Porous Graphitic Carbon Column and a Toluene–Isopropanol Gradient Mobile Phase

“…Table presents a comparison between PGC‐HPLC‐APCI/MS and NARP‐LC in terms of the maximum back pressure ( P max ), AT, number of peaks ( n ), and R smin . In recent studies, good experimental results were achieved using a phenyl‐hexyl column to profile TAG in plant oil (Hu et al, ; Wei et al, ). These works proposed a mixed‐mode retention mode of TAG separation, which can be considered a complementary explanation with the retention mode on the phenyl‐hexyl column.…”

“…Hu et al () developed an off‐line 2D system coupling NARP and the silver‐ion HPLC method for the identification and quantification of TAG in peanut oil. Recent studies reported on the profiling of TAG differing in both ECN and DB, as well as the partial resolution of regioisomers with a single column under optimized conditions (Hu et al, ; Wei et al, ; Wei, Hu, Lv, Dong, & Chen, ). In contrast to the NARP‐HPLC mode, Ag + ‐HPLC provides a good separation resulting from the weak reversible interaction between Ag + integrated in the silica stationary phase and the DB of TAG with π electrons.…”

Detection of Edible Plant Oil Adulteration by Triacylglycerol Profiles Using an Atmospheric Pressure Chemical Ionization Source and MS3 Ion Trap Mass Spectrometry

“…The markers used in these methods are low‐content components in edible plant oils. In contrast, triacylglycerol is the main components in edible plant oils, which account for 95–98% . However, identification of triacylglycerol in edible oils is a difficult and challenging task because of a large number of isomeric triacylglycerol.…”

Theory and Practice of UHPLC and UHPLC–MS

“…On the one hand, for NP  RP separation of carotenoids, either silica or cyanopropyl (CN) stationary phases have been investigated (Dugo et al , 2008d(Dugo et al ,e, 2009cCacciola et al, 2012Cacciola et al, , 2016a, whereas for triacylglycerol (TAG) separation, an Ag  RP combination turned out to be useful for this task Dugo et al, 2006b,c;Van der Klift et al, 2008;Hu et al, 2013;Wei et al, , 2015Costa et al, 2015;. On the other hand, for RP  RP separation of phenolic antioxidants, the effect of several stationary phase chemistries (C8, C18, CN, Diol, F 5 , NH 2 , PEG, Phenyl, RPAmide, and other laboratory-made stationary phases) on the separation selectivity and resolution has been investigated (Blahovà et al, 2006;Cacciola et al, 2006Cacciola et al, , 2007aCacciola et al, ,b,c, 2011Hyilompolo, 2007, 2008;Pol et al, 2007, Pol andDugo et al, 2008cDugo et al, , 2009aJandera et al, 2008aJandera et al, ,b, 2012Hájek et al, 2008Cesla et al, 2009;Kalili and de Villiers, 2009, 2013aZhou et al, 2009;Russo et al, 2011;Fu et al, 2012;Hájek and Jandera, 2012;Kalili et al 2013Kalili et al , 2014Montero et al, 2013aMontero et al, ,b, 2014Montero et al, , 2016aKrol-Kogus et al, 2014;Krauze-Baranowska et al, 2014;Leme et al, 2014;Tanaka et al, 2014;…”

“…A very recent approach for TAG analysis in edible oils has been reported by Wei et al ( , 2015: in such works novel mixed-mode single chromatographic columns were employed combining the features of traditional C8/C18 and silver-ion columns.…”