Quantitation of α2u-Globulin in Rat Kidney Cytosol by Capillary Electrophoresis

Pähler, Axel; Blumbach, Kai; Herbst, Jörg; Dekant, W.

doi:10.1006/abio.1998.3004

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…These applications include analysis of chemokine stromal cell-derived factor-1 [181], soluble chromatin from chicken erythrocytes [182], jellyfish venom proteins [183], sialoglycoproteins [184], turkey egg ovalbumin glycoforms [57], cathepsin D [185], bovine collagens [116], fluorescent adduct formed in vivo in collagen [71], caseinoglycomacropeptide in cosmetic lotions [186], polyethylene glycol-modified proteins (including hemoglobin as a potential blood substitute) [187], salmon calcitonin [188], ribonuclease and lysozyme [189], further transmembrane serine receptor [190], salivary a-amylase [191], outer membrane proteins from Proteus penneri [192], granulocyte colonystimulating factor [193], insulin antibodies [194], staphylococcal enterotoxin A [195], green fluorescent proteins in human embryonic kidney cells [196], peroxidase:antiperoxidase immune complexes [197], a(2u)-globulin in rat kidney cytosol [198], histone H1 variants [50], dilute proteins in formulations containing interfering excipients [199], subviral particles of human rhinovirus serotype 2 [200], seed 2S albumins [201], mutant a-and b-globulin chains [202], abnormal prion proteins [203] proteins interacting with DNA [204], alkaline phosphatase inhibited by theophylline [205], proteins in single cells [70], determination of Src kinase and kinase C activity [206], and bilirubin oxidase activity [207].…”

Section: Other Proteinsmentioning

confidence: 99%

Capillary electrophoresis of proteins 1999-2001

Dolnı́k¹,

Hutterer²

2001

Electrophoresis

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This review article with 223 references describes recent developments in capillary electrophoresis (CE) of proteins and covers papers published during last two years, from the previous review (V. Dolnik, Electrophoresis 1999, 20, 3106-3115) through Spring 2001. It describes the topics related to CE of proteins including modeling of the electrophoretic properties of proteins, sample pretreatment, wall coatings, improving selectivity, detection, special electrophoretic techniques, and applications.

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Section: Other Proteinsmentioning

confidence: 99%

Capillary electrophoresis of proteins 1999-2001

Dolnı́k¹,

Hutterer²

2001

Electrophoresis

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“…Radioimmunoassay showed that, relative to total protein in liver, α2u-globulin ultimately rose to a maximum ~ 3.5 ng/mg at 70 days old. Subsequently, α2u-globulin was isolated from kidney cytosol of adult Fischer male rats and remained a single entity in SDS-PAGE and capillary electrophoresis [12], but occurred in greater concentration in kidney of rats given agents that induce α2u-globulin-nephropathy. However, α2u-globulin had already been described as resolvable electrophoretically into two distinct molecular forms [13].…”

Section: Introductionmentioning

confidence: 99%

Changes in male rat urinary protein profile during puberty: a pilot study

et al. 2013

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BackgroundAndrogen-dependent proteins (lipocalins) circulate in blood of male rats and mice and, being small (~ 18 kDa), pass freely into glomerular filtrate. Some are salvaged in proximal nephrons but some escape in urine. Several organic molecules can bind to these proteins causing, where salvage occurs, nephropathy including malignancy in renal cortex. In urine, both free lipocalins and ligands contribute to an increasingly-recognised vital biological role in social communication between adults, especially in the dark where reliance is on smell and taste. Crystal structure of the first-characterised lipocalin of male rats, α2u-globulin, has been determined and peptide sequences for others are available, but no study of occurrence during early puberty has been made. We have followed temporal occurrence in urine of juveniles (n = 3) for non-invasive pilot study by high resolution gradient mini-gel electrophoresis, tryptic digest of excised protein bands, and LC-MS/MS of digest to identify peptide fragments and assign to specific lipocalins. Study objective refers directly to external availability for social communication but also indirectly to indicate kinetics of circulating lipocalins to which some xenobiotics may bind and constitute determinants of renal disease.ResultsMini-gels revealed greater lipocalin complexity than hitherto recognised, possibly reflecting post-translational modifications. Earliest patterns comprised rat urinary protein 1, already evident in Sprague-Dawley and Wistar strains at 36 and 52 days, respectively. By 44 and 57 days major rat protein (α2u-globulin) occurred as the progressively more dominant protein, though as two forms with different electrophoretic mobility, characterised by seven peptide sequences. No significant change in urinary testosterone had occurred in Wistars when major rat protein became evident, but testosterone surged by 107 days concomitant with the marked abundance of excreted lipocalins.ConclusionsQualitative temporal changes in the composition of excreted lipocalins early in puberty, and apparent increase in major urinary protein as two resolvable forms, should catalyse systematic non-invasive study of urinary lipocalin and testosterone dynamics from early age, to illuminate this aspect of laboratory rodent social physiology. It could also define the potential temporal onset of nephrotoxic ligand risk, applicable to young animals used as toxicological models.

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“…Allerdings ist der Mechanismus der Tumorigenität bislang nicht bekannt. Eine metabolische Aktivierung von TCEP durch Glutathion konnte ebenso ausgeschlossen werden wie eine relevante Bildung von α 2µ -Globulin [36].…”

Section: Kanzerogenität Und Gentoxizitätunclassified