Quantitative analysis of acetylsalicylic acid in human blood using volumetric absorptive microsampling

Kim, Yun-Jeong; Jeon, Ji‐Young; Han, Songhee; Ha, Na; Jang, Kyungho; Kim, Min‐Gul

doi:10.12793/tcp.2018.26.1.32

Cited by 6 publications

(5 citation statements)

References 18 publications

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“…This is mainly done by drug screening via blood or urine chemical tests. The most widely used methods for detection of pharmaceuticals or their metabolites in blood are liquid chromatography tandem mass spectrometry and gas chromatography coupled with mass spectrometry . These methods are quite sensitive and allow the detection of SA with concentration down to 0.04 mg/l (30 μM).…”

Section: Introductionmentioning

confidence: 99%

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Adomaviciute

Velička

Šablinskas

2020

J Raman Spectroscopy

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Aspirin being freely available as an over the counter drug has a high overdose risk and can cause severe health symptoms. The methods used in clinics for the detection of such drugs (pharmaceuticals) in bodily fluids are mostly based on liquid chromatography and mass spectroscopy. These methods are known to be precise; however, they both require long and laborious sample preparation; thus, it takes time to acquire the required information. Because in the case of an overdose, the time has high importance, and faster methods would be beneficial. This work presents an application of a Surface‐enhanced Raman scattering (SERS) spectroscopic method for the detection of salicylic acid as a metabolite of aspirin in the blood serum of the patient under examination. In this research, the various colloidal solutions were employed for the preparation of SERS active substrates. The choice of the most efficient colloidal solution and the challenges of collecting SERS spectra of whole blood or its components are discussed. The spectra of both the model and the real‐life blood samples containing metabolite of aspirin were collected with a Fourier transform Raman spectrometer. The analysis of the collected spectra revealed that label‐free SERS can be used for the detection of salicylic acid with concentration down to 3 mM in blood serum corresponding to consumption of at least eight standard pills of aspirin (equivalent to mild toxicity). The proposed diagnostic method could be applied faster than the standard methods and could allow sensitive and fast diagnosis of aspirin consumption in the human blood.

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Section: Introductionmentioning

confidence: 99%

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Adomaviciute

Velička

Šablinskas

2020

J Raman Spectroscopy

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“…Different studies have not specified the time of the tip’s contact with the blood [ 24 , 31 ], or they used similar times as used in this study. However, it was preferred to establish a fixed time of 7 s to ensure full absorption to obtain a standardized protocol, as also used by [ 30 , 32 , 33 , 34 ]). After the tip was dried, it is generally recommended to wait ≥2–3 h to consider a tip as dry [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Vidal

Belova

Stove

et al. 2021

Toxins

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Biomonitoring of biological samples arises as an effective tool to evaluate the exposure to mycotoxins in the population. Owing to the wide range of advantages, there is a growing interest in the use of non- and minimally invasive alternative sampling strategies, such as dried blood spot sampling or volumetric absorptive microsampling (VAMS). A VAMS-based multi-mycotoxin method was developed and validated for 24 different mycotoxins. Method validation was based on the Bioanalytical Method Validation Guideline of the Food and Drug Administration from the United States and for most of the studied mycotoxins, the results of the performance characteristics were in agreement with the criteria of the European Commission Decision 2002/657/EC. The recovery for the different mycotoxins was not haematocrit dependent and remained acceptable after storing the VAMS for 7 and 21 days at refrigeration temperature (4 °C) and room temperature, demonstrating that VAMS could be applied to assess mycotoxin exposure in blood in resource-limited areas, where there may be a delay between sampling and analysis. Finally, a comparison between VAMS and a procedure for liquid whole blood analysis, performed on 20 different blood samples, did not result in missed exposed cases for VAMS. Moreover, both methods detected similar levels of ochratoxin A, ochratoxin alpha, zearalenone and aflatoxin B1. Given all the benefits associated with VAMS and the developed method, VAMS sampling may serve as an alternative to conventional venous sampling to evaluate multiple mycotoxin exposure.

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“…Typically, the devices are weighed before and after filling to determine the volume of blood collected within the VAMS tip by considering the density of the matrix. This volume sampling test was carried out for the determination of acetylsalicylic acid by Kim et al using the given formulae 35 :…”

Section: Sampling Parameters Sample Volumementioning

confidence: 99%

Applications of Volumetric Absorptive Microsampling Technique: A Systematic Critical Review

Dodeja

Giannoutsos

Caritis

et al. 2023

Therapeutic Drug Monitoring

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Background:Microsampling is a technique that utilizes a very small volume of biological matrix, typically less than 50 µL, for quantitatively analyzing chemicals, drugs, and biologics. The use of filter paper for blotting blood samples, usually referred to “Dried Blood Spots”, is an example of a microsampling approach, which has been established since the early 1960s. However, non-homogeneous sample spread, the influence of hematocrit, improper volume measurement, and environmental degradation are major bottlenecks to the widespread adoption of this technique in routine clinical sample analysis. Novel methods have overcome several of these issues; however, assays using these microsampling tools involve variable steps/approaches.Methods:A novel microsampling device called Volumetric Absorptive microsampling (VAMS), developed in 2014, appears to have resolved the sample inhomogeneity inherent to dried blood spots, with improved precision in the volume of sample collected for measuring drug concentration. A literature search was conducted to identify several analytical and pharmacokinetic studies that have used VAMS in recent years.Results:The key factors for proper experimental design and optimization of the extraction of drugs and metabolites of interest from the device were summarized. This review focuses on VAMS and elaborates on bioanalytical factors, method validation steps, and scope of this technique in clinical practice.Conclusions:The promising microsampling method VAMS is especially suited for conducting pharmacokinetic studies with very small volumes of blood, especially in special patient populations. Clinical validation of every VAMS assay must be conducted prior to the routine practical implementation of this method.

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Quantitative analysis of acetylsalicylic acid in human blood using volumetric absorptive microsampling

Cited by 6 publications

References 18 publications

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Applications of Volumetric Absorptive Microsampling Technique: A Systematic Critical Review

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