Quantitative Analysis of Allantoin in <i>Dioscorea japonica</i> Peel Using an Amino Bonded-Phase HPLC Column

Quantification of Allantoin in Yams (Dioscorea sp.) Using a 1H NMR Spectroscopic Method

“…On the contrary, 1 H NMR is a fast analysis, highly reproducible, and robust quantitative technique [5,22]. We first quantitated allantoin from eight yam extracts using the HPLC-photodiode array (PDA) method which was reported recently [19]. The chromatograms were obtained at 235 nm.…”

“…Several methods, including high-performance liquid chromatography (HPLC) methods, for allantoin determination, have been studied [16]. Nevertheless, no information is known in the literature on qNMR methods dealing with the analysis of allantoin in yams up to now [16][17][18][19]. Our previous study has revealed a quantitative analysis method using HPLC for allantoin identification in the peel of Dioscorea japonica; however, the method is limited Allantoin is an abundant component of yams and has been known as a skin protectant due to its pharmacological activities.…”

“…in evaluating allantoin in the different matrices of yam [19]. The aim of this study was to develop a rapid, sensitive, and reliable 1 H qNMR spectroscopy-based method for the allantoin quantitation in yams.…”