Quantitative Analysis of Amygdalin and Prunasin in Prunus serotina Ehrh. using ¹H‐NMR Spectroscopy

Abstract

The NMR method offers a fast, high-throughput analysis of cyanogenic glycosides in dried leaves permitting simultaneous quantification and identification of prunasin and amygdalin in Prunus serotina.

Ameliorative effects of vitamin B17 on the kidney against Ehrlich ascites carcinoma induced renal toxicity in mice

“…Vitamin B17 (VB17), amygdalin and formerly called laetrile is one of many nitrilosides which are natural cyanide containing substances, which is abundant in the seeds of Prunasins family, such as apricots, apples, almonds, peaches, and other rosaceous plants . VB17 consists of two molecules of glucose, one of them is benzaldehyde which induces an analgesic action, the second is hydrocyanic acid which is an antineoplastic compound and used to treat the tumor and relieve pain …”

Amygdalin Regulates Apoptosis and Adhesion in Hs578T Triple-Negative Breast Cancer Cells

“…Amygdalin, D-mandelonitrile-β-D-glucoside-6-β-glucoside, belongs to the aromatic cyanogenic glycoside group and is widely distributed in the rosaceous plant seed, for example, apricot, peach, apple, cherry, plum, etc . ( Holzbecher et al ., 1984 ; Santos Pimenta et al ., 2014 ). Amygdalin is known for effective component of the traditional Chinese medicine and used as auxiliary medicine of cough expectorant agent and cancer therapy ( Holland, 1982 ; Milazzo et al ., 2011 ).…”

Quantitative NMR Studies of Multiple Compound Mixtures

“…Aside from as a major analytical tool for structure elucidation of unknown natural products, NMR is also widely used to quantitatively analyze natural products [122] (see Table 2), including (a) small molecular weight chemical compounds such as alcohols [133,134], phenolic acids [125,126], glycosides [124,129], alkaloids [127,130], flavonolignans [131], alkylene [132], heterocyclic compounds [128,141], and terpenoids [123,132] and (b) high molecular weight natural substances such as polysaccharides [142] and lignins [135][136][137][138][139][140]. To obtain accurate quantitative results, it is usually required to optimize qNMR toward given class of compounds.…”