Quantitative Analysis of Autophagic Flux by Ratiometric pH-Imaging of Autophagic Intermediates

Late-stage functionalisation of alkyne-modified phospha-xanthene dyes: lysosomal imaging using an off–on–off type of pH probe

“…This dextran-conjugated probe will be an important tool for the understanding of important biological events such as autophagy. 50,51 Throughout this research we have demonstrated a unique way of modifying phospha-xanthene dyes, which we believe will open up new avenues to create various types of useful NIR-emissive probes.…”