Quantitative analysis of crystalline style carbohydrases in five suspension- and deposit-feeding bivalves

Brock, V.; Kennedy, Victor S.

doi:10.1016/0022-0981(92)90257-b

Cited by 20 publications

(15 citation statements)

References 14 publications

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“…Similar to clams fed with the control diet, amylase and cellulase complex were the most active enzymes in the digestive glands of T. dombeii fed toxic algae, while laminarinase activity remained lower than the activities of the other enzymes. This finding agrees with Brock and Kennedy (1992) who reported high laminarinase levels in style extracts of C. virginica, whereas low laminarinase activity was reported in oyster diverticula extracts (Brock et al 1986).…”

Section: Discussionsupporting

confidence: 92%

Enzymatic digestive activity and absorption efficiency in Tagelus dombeii upon Alexandrium catenella exposure

et al. 2013

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We analyzed absorption efficiency (AE) and digestive enzyme activity (amylase, cellulase complex, and laminarinase) of the infaunal bivalve Tagelus dombeii originating from two geographic sites, Corral-Valdivia and Melinka-Aysén, which have different long-term paralytic shellfish poisoning (PSP) exposure rates. We report the effects of past feeding history (origin) on T. dombeii exposed to a mixed diet containing the toxic dinoflagellate Alexandrium catenella and another dinoflagellate-free control diet over a 12-day period in the laboratory. Absorption efficiency values of T. dombeii individuals that experienced PSP exposure in their habitat (Melinka-Aysén) remained unchanged during exposure to toxic food in the laboratory. In contrast, T. dombeii from a non-PSP exposure field site (Corral-Valdivia) showed a significant reduction in AE with toxic exposure time. This study established that the amylase and cellulase complexes were the most important enzymes in the digestive glands of Tagelus from both sites. The temporal evolution of enzymatic activity under toxic diet was fitted to exponential (amylase and cellulase) and to a logarithmic (laminarinase) models. In all fits, we found significant effect of origin in the model parameters. At the beginning of the experiment, higher enzymatic activity was observed for clams from Corral-Valdivia. The amylase activity decreased with time exposure for individuals from Corral and increased for individuals from Melinka. Cellulase activity did not vary over time for clams from Corral, but increased for individuals from Melinka and laminarinase activity decreased over time for individuals from Corral and remained unchanged over time for Melinka. A feeding history of exposure to the dinoflagellate A. catenella was reflected in the digestive responses of both T. dombeii populations.

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Section: Discussionsupporting

confidence: 92%

Enzymatic digestive activity and absorption efficiency in Tagelus dombeii upon Alexandrium catenella exposure

et al. 2013

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“…Riera & Richard 1996, Chanton & Lewis 2002) since the material is less nutritive than microalgal sources (Kristensen 1972). However, recent studies have shown that some benthic animals have a cellulase (Brock & Kennedy 1992, Sakamoto et al 2007) that allows the organisms to use terrestrial plant material as a major carbon source (Kasai & Nakata 2005, Kasai et al 2006. These findings imply a direct linkage between the terrestrial and marine food webs (i.e.…”

Section: Introductionmentioning

confidence: 72%

“…In contrast, L. marilina assimilated terrestrial plant materials that are rich in indigestible cellulose and lignin (Hargrave 1970, Kristensen 1972, Tenore 1983). Some bivalves have recently been reported to have a cellulase and can directly assimilate plant detritus (Brock & Kennedy 1992, Kasai & Nakata 2005, Sakamoto et al 2007). Sakamoto et al (2007) showed that Corbicula japonica, a common brackish bivalve species in Japan, has an endogenous cellulase.…”

Section: Major Diets Of Consumers In the River-dominated Lagoon (Idoura)mentioning

confidence: 99%

“…In general, cyanobacteria cell walls are rich with mucopolysaccharides and indigestible to aquatic animals (Hargrave 1970). However, some aquatic organisms have specific enzymes (Kristensen 1972, Brock & Kennedy 1992) that account for the dietary separation of sympatric bivalve species in estuarine habitats (Kasai & Nakata 2005). L. marilina may have some physiological characteristics (e.g.…”

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confidence: 99%

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Spatial dietary variations in Laternula marilina (Bivalva) and Hediste spp. (Polychaeta) along environmental gradients in two brackish lagoons

Kanaya¹,

Takagi²,

Kikuchi³

2008

Mar. Ecol. Prog. Ser.

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“…The crystalline style is an extracellular structure existing in the stomach of bivalves, and is composed mainly of digestive enzymes including several carbohydrate-splitting enzymes (Gosling, 2003;Brock and Kennedy, 1992). By detailed comparison of the enzymatic activities in the crystalline style, we biochemically examined the possibility that C. japonica can assimilate plant-derived cellulose and hemicelluloses.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of cellulase and hemicellulase activities of brackish water clam Corbicula japonica with those of other marine Veneroida bivalves

Sakamoto

Toyohara

2009

Journal of Experimental Biology

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SUMMARYCorbicula japonica is a typical brackish water bivalve species belonging to the order Veneroida, and it is the most important inland fishery resource in Japan. Corbicula japonica has been suggested to assimilate organic matter from terrestrial plants, unlike Ruditapes philippinarum and Mactra veneriformis, which selectively assimilate organic matter of marine origin. This led us to hypothesize that C. japonica, despite being a suspension feeder, could assimilate cellulosic materials derived from terrestrial plants. In the present study, we measured cellulase and hemicellulase activities in the crystalline styles of C. japonica and other commercially important Veneroida bivalve species in Japan: Ruditapes philippinarum, Meretrix lamarckii and Meretrix lusoria. Corbicula japonica demonstrated notably higher cellulase, xylanase and β-mannanase activities than the other marine bivalves, suggesting that this species possesses a far greater biochemical capacity to break down the structural polysaccharides of plant cell walls than the other species. In contrast, the β-1,3-glucanase and pectinase activities of C. japonica were similar to or even lower than those of the others. This is possibly due to the presence of these polysaccharides in the cell walls of diatoms, a principal food of most marine bivalves. Although direct evidence is lacking, the high cellulase, xylanase and β-mannanase activities of C. japonica may result from adaptation to an upstream estuarine environment where phytoplankton and diatoms are scarce, but plant-derived substances are abundant.

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Quantitative analysis of crystalline style carbohydrases in five suspension- and deposit-feeding bivalves

Cited by 20 publications

References 14 publications

Enzymatic digestive activity and absorption efficiency in Tagelus dombeii upon Alexandrium catenella exposure

Enzymatic digestive activity and absorption efficiency in Tagelus dombeii upon Alexandrium catenella exposure

Spatial dietary variations in Laternula marilina (Bivalva) and Hediste spp. (Polychaeta) along environmental gradients in two brackish lagoons

A comparative study of cellulase and hemicellulase activities of brackish water clam Corbicula japonica with those of other marine Veneroida bivalves

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