Quantitative analysis of GABAergic neurons in the mouse hippocampus, with optical disector using confocal laser scanning microscope

Jinno, Shozo; Aika, Yusuke; Fukuda, Takahiro; Kosaka, Toshio

doi:10.1016/s0006-8993(98)01075-0

Cited by 101 publications

(110 citation statements)

References 48 publications

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“…The dissector height was 20 m. Only cell bodies that met the counting criteria according to the optical dissector principle within the counting frame were counted; cells within the counting frame that touch the red (lower and left) boundaries were excluded. The total number of cells counted was multiplied by 68.6 (1/12 for sections analyzed; 1 ⁄ 4 counting area and 70/100 tissue shrink factor (29). In all cases optimum sampling was achieved as the coefficient of error (CE) is lower than the coefficient of variation (30).…”

Section: Methodsmentioning

confidence: 99%

Cognitive Deficits and Disruption of Neurogenesis in a Mouse Model of Apolipoprotein E4 Domain Interaction

Adeosun

Hou

Zheng

et al. 2014

Journal of Biological Chemistry

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Background: Domain interaction may be the principal pathogenic feature of apoE4 in Alzheimer disease. Results: Young and old mice with mutations causing apoE4 domain interaction showed impaired cognition and a disruption in neurogenesis. Conclusion: Domain interaction mediates apoE4 neuro-pathologies and cognitive phenotype. Significance: Domain interaction is a viable prophylactic target against apoE4 cognitive phenotype and increased susceptibility to Alzheimer disease.

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Section: Methodsmentioning

confidence: 99%

Cognitive Deficits and Disruption of Neurogenesis in a Mouse Model of Apolipoprotein E4 Domain Interaction

Adeosun

Hou

Zheng

et al. 2014

Journal of Biological Chemistry

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“…Staining for GAD67 immunoreactivity revealed a higher numerical density and cell size of inhibitory neurons in the temporal third of the hippocampus compared with the septal third. When separated by laminar subdivision, this septotemporal difference persists in both CA3 and DG, but not CA1 (Jinno et al 1998). The number of g-aminobutyric acid (GABA)ergic interneurons immunoreactive for somatostatin, cholecystokinin, and vasoactive intestinal polypeptide was also higher in the temporal hippocampus .…”

Section: Molecular Markersmentioning

confidence: 99%

Functional Differentiation of Adult-Born Neurons along the Septotemporal Axis of the Dentate Gyrus: Figure 1.

Sahay

Duman

et al. 2015

Cold Spring Harb Perspect Biol

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Over the past several decades, the proliferation and integration of adult-born neurons into existing hippocampal circuitry has been implicated in a wide range of behaviors, including novelty recognition, pattern separation, spatial learning, anxiety behaviors, and antidepressant response. In this review, we suggest that the diversity in behavioral requirements for new neurons may be partly caused by separate functional roles of individual neurogenic niches. Growing evidence shows that the hippocampal formation can be compartmentalized not only along the classic trisynaptic circuit, but also along a longitudinal septotemporal axis. We suggest that subpopulations of hippocampal adult-born neurons may be specialized for distinct mnemonic-or mood-related behavioral tasks. We will examine the literature supporting a functional and anatomical dissociation of the hippocampus along the longitudinal axis and discuss techniques to functionally dissect the roles of adult-born hippocampal neurons in these distinct subregions.

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“…It is known that distinct subpopulations of inhibitory interneurons are located in different locations of the CA3 region or dentate gyrus (Spruston et al, 1997;Jinno et al, 1998;Gaïarsa et al, 2001;Aaron et al, 2003;Jonas et al, 2004). Among them, a subset of interneurons so called mossy fiber-associated (MFA) interneurons resides in the stratum lucidum along the mossy fiber projection, and their axons spread over to the hilus, close to the dentate gyrus granule cell layer (Vida and Frotscher, 2000;Losonczy et al, 2004).…”

Section: Purely Glutamatergic Phenotype Of Mossy Fiber Terminals In Dmentioning

confidence: 99%

Evidence against GABA Release from Glutamatergic Mossy Fiber Terminals in the Developing Hippocampus

Uchigashima

Fukaya²,

Watanabe³

et al. 2007

J. Neurosci.

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Hippocampal mossy fibers of young rodents have been reported to corelease inhibitory neurotransmitter GABA in addition to excitatory transmitter glutamate. In this study, we aimed at re-evaluating this corelease hypothesis of both inhibitory and excitatory transmitters in the hippocampus. Electrophysiological examination revealed that, in juvenile mice and rats of the two to 3 weeks old, stimulation at the granule cell layer of the dentate gyrus elicited monosynaptic GABAergic IPSCs in CA3 neurons in the presence of ionotropic glutamate receptor (iGluR) blockers, only when rather strong stimuli were given. The group II mGluR agonist (2S,1ЈR,2ЈR,3ЈR)-2-(2,3-dicarboxycyclo-propyl)glycine (DCG-IV), which selectively suppresses transmission at the mossy fiber-CA3 synapse, abolished almost all postsynaptic responses elicited by the weak stimuli, whereas those by strong stimuli were inhibited only slightly. In addition, the minimal stimulation elicited GABAergic IPSCs in neonatal mice of the first postnatal week, whereas these responses are not sensitive to DCG-IV. Immunohistochemical examination revealed that mossy fiber terminals expressed GABA and the GABA-synthesizing enzyme GAD67, although the expression levels were much weaker than those in the inhibitory interneurons. Notably, the expression levels of the vesicular GABA transporter were much lower than those of GABA and GAD67, and almost below detection threshold. These results suggest that mossy fiber synapses are purely glutamatergic and apparent monosynaptic IPSCs so far reported are evoked by costimulation of inhibitory interneurons, at least in young mice and rats. Hippocampal mossy fiber terminals synthesize and store GABA, but have limited ability in vesicular release for GABA in the developing rodents.

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Quantitative analysis of GABAergic neurons in the mouse hippocampus, with optical disector using confocal laser scanning microscope

Cited by 101 publications

References 48 publications

Cognitive Deficits and Disruption of Neurogenesis in a Mouse Model of Apolipoprotein E4 Domain Interaction

Cognitive Deficits and Disruption of Neurogenesis in a Mouse Model of Apolipoprotein E4 Domain Interaction

Functional Differentiation of Adult-Born Neurons along the Septotemporal Axis of the Dentate Gyrus: Figure 1.

Evidence against GABA Release from Glutamatergic Mossy Fiber Terminals in the Developing Hippocampus

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