2014
DOI: 10.1016/j.ab.2014.08.005
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Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography–electrospray ionization–tandem mass spectrometry

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Cited by 47 publications
(28 citation statements)
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References 35 publications
(42 reference statements)
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“…Promising results have been reported for the relationship between gagCEST and GAG concentration through experiments where tissue is chemically degraded using trypsin . Our results do not contradict the results of these earlier studies since these experiments were conducted in samples of animal cartilage (typically young and thus likely to contain a larger amount of CS‐A). Also, in these studies the same cartilage sample was followed over time and the CS‐A to CS‐C ratio probably did not change during trypsin treatment.…”
Section: Discussionsupporting
confidence: 78%
“…Promising results have been reported for the relationship between gagCEST and GAG concentration through experiments where tissue is chemically degraded using trypsin . Our results do not contradict the results of these earlier studies since these experiments were conducted in samples of animal cartilage (typically young and thus likely to contain a larger amount of CS‐A). Also, in these studies the same cartilage sample was followed over time and the CS‐A to CS‐C ratio probably did not change during trypsin treatment.…”
Section: Discussionsupporting
confidence: 78%
“…The biochemical method used for GAGs analysis in dried blood spots has previously been described [12,17]. Initial validation [18] showed a clear differentiation of known MPS I cases from cases carrying pseudodeficiency alleles.…”
Section: Analytical Methods and Reference Populationmentioning
confidence: 99%
“…To quantify GAGs in different matrices, liquid chromatography tandem mass spectrometry has been used [48]. GAGs can be quantified in: urine [34,[49][50][51][52][53][54], serum/plasma [34,48,[54][55][56], dried blood spots [57][58][59] (Figure 3), amniotic fluid [60], cerebrospinal fluid [34,61], cultured cells [34], and tissues [62]. Some of these assays can be used for newborn screening of MPSs [57,63] or even to allow the discrimination of specific disease subtypes [53].…”
Section: Biomarkersmentioning
confidence: 99%