Quantitative analysis of hindlimbs locomotion kinematics in spinalized rats treated with Tamoxifen plus treadmill exercise

Osuna-Carrasco, Laura Paulina; López-Ruiz, Jose Roberto; Mendizabal-Ruiz, Gerardo; Torre-Valdovinos, Braniff De la; Bañuelos-Pineda, Jacinto; Jiménez‐Estrada, Ismael; Dueñas‐Jiménez, Sergio Horacio

doi:10.1016/j.neuroscience.2016.07.023

Cited by 15 publications

(11 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the curves that can be employed to perform a comparison of hind limbs locomotion is the leg pendulum-like movement (PLM) of the hind limb which corresponds to the angle between the the 5th lumbar vertebra and fifth metatarsophalangeal joint (first and last points) [7]. In this work, we computed this angle for each frame, interpolated missing angles in case of non convergence of the algorithm, and then applied a robust weighted moving average filter with an outliers parameter of .…”

Section: Methodsmentioning

confidence: 99%

“…In particular, the analysis of locomotion in laboratory rats allows us to understand the motor defects in diseases such as osteoarthrosis [4], Parkinson’s disease, Huntington’s disease, and amyotrophic lateral sclerosis [5, 6], as well as the damage and recovery after peripheral and central nervous system injury [7]. …”

Section: Introductionmentioning

confidence: 99%

“…Current methodologies for the analyses of laboratory rats’ locomotion can be categorized as “forced” when the speed of march is imposed on the rat by means of a treadmill (e.g., [8]), or “unforced” when the rat is free to move at any speed on the ground or an activity wheel [7]. In general, forced analysis have the advantage of allowing to perform direct comparisons between the recollected gait variables, since all of the subjects will be moving at similar speeds.…”

Section: Introductionmentioning

confidence: 99%

“…These approaches rely on the use of video sequence recording at high speed (i.e., in general above 90 frames per second) that are analyzed through the inspection of the angles between the joints of the extremity of interest obtained by manual annotations of the extremity along the recorded frames. The curves corresponding to changes of the joint angles with respect to time can then be used to compare the gait patterns of different experimental rat groups [7]. …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Modeling hind-limb kinematics using a bio-inspired algorithm with a local search

et al. 2018

View full text Add to dashboard Cite

BackgroundLaboratory rats play a critical role in research because they provide a biological model that can be used for evaluating the affectation of diseases and injuries, and for the evaluation of the effectiveness of new drugs and treatments. The analysis of locomotion in laboratory rats facilitates the understanding of motor defects in many diseases, as well as the damage and recovery after peripheral and central nervous system injuries. However, locomotion analysis of rats remains a great challenge due to the necessity of labor intensive manual annotations of video data required to obtain quantitative measurements of the kinematics of the rodent extremities. In this work, we present a method that is based on the use of a bio-inspired algorithm that fits a kinematic model of the hind limbs of rats to binary images corresponding to the segmented marker of images corresponding to the rat’s gait. The bio-inspired algorithm combines a genetic algorithm for a group of the optimization variables with a local search for a second group of the optimization variables.ResultsOur results indicate the feasibility of employing the proposed approach for the automatic annotation and analysis of the locomotion patterns of the posterior extremities of laboratory rats.ConclusionsThe adjustment of the hind limb kinematic model to markers of the video frames corresponding to rat’s gait sequences could then be used to analyze the motion patterns during the steps, which, in turn, can be useful for performing quantitative evaluations of the effect of lesions and treatments on rats models.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modeling hind-limb kinematics using a bio-inspired algorithm with a local search

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The values in centimeters were introduced into a software platform designed by CINVESTAV-IPN (17), which associates joints defined by Cartesian coordinates, drawing all the resulting lines of the movement executed by the animal's limbs during gait and the movement sequence of the extremities during walking.…”

Section: Kinematic Analysismentioning

confidence: 99%

Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury

et al. 2020

View full text Add to dashboard Cite

The regenerative capability of the central nervous system is limited after traumatic spinal cord injury (SCI) due to intrinsic and extrinsic factors that inhibit spinal cord regeneration, resulting in deficient functional recovery. It has been shown that strategies, such as pre-degenerated peripheral nerve (PPN) grafts or the use of bone marrow stromal cells (BMSCs) or exogenous molecules, such as chondroitinase ABC (ChABC) promote axonal growth and remyelination, resulting in an improvement in locomotor function. These treatments have been primarily assessed in acute injury models. The aim of the present study is to evaluate the ability of several single and combined treatments in order to modify the course of chronic complete SCI in rats. A complete cord transection was performed at the T9 level. One month later, animals were divided into five groups: original injury only (control group), and original injury plus spinal cord re-transection to create a gap to accommodate BMSCs, PPN, PPN + BMSCs, and PPN + BMSCs + ChABC. In comparison with control and single-treatment groups (PPN and BMSCs), combined treatment groups (PPN + BMSCs and PPN + BMSCs + ChABC) showed significative axonal regrowth, as revealed by an increase in GAP-43 and MAP-1B expression in axonal fibers, which correlated with an improvement in locomotor function. In conclusion, the combined therapies tested here improve locomotor function by enhancing axonal regeneration in rats with chronic SCI. Further studies are warranted to refine this promising line of research for clinical purposes.

show abstract

A System for High-Speed Synchronized Acquisition of Video Recording of Rodents During Locomotion

et al. 2019

View full text Add to dashboard Cite

Quantitative analysis of hindlimbs locomotion kinematics in spinalized rats treated with Tamoxifen plus treadmill exercise

Cited by 15 publications

References 40 publications

Modeling hind-limb kinematics using a bio-inspired algorithm with a local search

Modeling hind-limb kinematics using a bio-inspired algorithm with a local search

Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury

A System for High-Speed Synchronized Acquisition of Video Recording of Rodents During Locomotion

Contact Info

Product

Resources

About