Quantitative analysis of immunocompetent cells in human periapical lesions: Correlations with clinical findings of the involved teeth

Matsuo, Takashi; Ebisu, Shigeyuki; Shimabukuro, Yoshio; Ohtake, Tsuyoshi; Okada, Hiroshi

doi:10.1016/s0099-2399(06)81350-6

Cited by 48 publications

(34 citation statements)

References 15 publications

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“…Although the reasons for proliferation of epithelium in this disease are not clear, it is always connected with local accumulation of various types of immune cells 17 . The fact that PG with altered proliferating epithelium has larger number of immune cells than those of PG containing only the rests of Malassez correlates with this hypothesis 17 . APs are part of the innate local host response of multicellular organisms, such as plants, insects, amphibians, birds and mammals 18 .…”

Section: Discussionmentioning

confidence: 99%

Periodontal study of β-defensin 4 immunolocalization in the epithelium of human radicular cysts

Caltabiano¹,

Musumeci²,

Leonardi³

et al. 2013

OA Anatomy

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Section: Discussionmentioning

confidence: 99%

Periodontal study of β-defensin 4 immunolocalization in the epithelium of human radicular cysts

Caltabiano¹,

Musumeci²,

Leonardi³

et al. 2013

OA Anatomy

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“…Las lesiones periapicales (LPAs) se desarrollan como resultado de la activación de la respuesta inmune frente a la estimulación antigénica continua proveniente de la infección bacteriana y de los restos pulpares necróticos en los canales radiculares. Esta respuesta del hospedero involucra la producción de citoquinas proinflamatorias que inducen reabsorción ósea con la formación de tejido de granulación infiltrado por linfocitos, células plasmáticas, macrófagos, polimorfonucleares neutrófilos (PMN) y mastocitos (5) . Entre los mediadores pro inflamatorios, el Factor de Necrosis Tumoral alfa (TNF-) se ha vinculado con el proceso de reabsorción de la matriz ósea.…”

Section: Introductionunclassified

Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática

Flores

Vitali

Cabrera

et al. 2011

Rev. Clin. Periodoncia Implantol. Rehabil. Oral

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RESUMENIntroducción: Como consecuencia de necrosis pulpar séptica, el ingreso de productos bacterianos en el periápice induce la producción de citoquinas pro-inflamatorias como el Factor de Necrosis Tumoral (TNF)-, que entre otras funciones participa en la diferenciación y activación de los osteoclastos para inducir reabsorción ósea, fenómeno característico de la periodontitis apical asintomática (PAA). El fluido crevicular gingival (FCG) ofrece un gran potencial como fuente de factores asociados con la actividad osteoclástica. El objetivo del presente estudio fue determinar los niveles de TNFen FCG de dientes con PAA y controles sanos contralaterales. Métodos: Se incluyeron 14 pacientes en la Clínica de Endodoncia de la Facultad de Odontología de la Universidad de Chile con diagnóstico clínico de PAA y se obtuvieron muestras de FCG con tiras de papel a partir de los dientes afectados y sanos contralaterales por un periodo estandarizado de 30 segundos. Se determinaron las concentraciones de proteínas totales mediante el método del ácido bisciconitico y los niveles de TNF-, mediante ensayo ELISA. Los datos se analizaron con Test-t pareado utilizando el programa StataV11. Resultados: Se detectaron niveles de TNF-significativamente mayores en el FCG de dientes con PAA estandarizados, tanto por 30 segundos de toma de muestra como por mg de proteínas totales. Conclusiones: Este estudio provee evidencia preliminar de que los niveles de TNF-en el FCG reflejan la presencia de PAA y podría ser de utilidad como complemento al diagnóstico clínico y monitoreo del estado de salud o enfermedad de los tejidos perirradiculares. ABSTRACT Introduction: As a consequence of septic pulp necrosis, the entry of bacterial products into periapical tissues induces the release of pro-inflammatory cytokines, such as the tumor necrosis factor (TNF)-. This pleiotropic cytokine is involved in the differentiation and activation of osteoclasts to induce bone resorption, a hallmark of asymptomatic apical periodontitis (AAP). Gingival crevicular fluid (GCF) has a great potential as a source of factors associated with osteoclastic activity. The aim of this study was to determine the levels of TNF-in GCF of teeth with PAA and contralateral healthy controls. Methods: A total of 14 patients with clinical diagnosis of AAP were enrolled from the Clinic of Endodontics, Faculty of Dentistry, Universidad de Chile during 2009 and 30-second GCF samples were obtained with paper strips from AAP teeth and contralateral healthy controls. Total protein concentration and TNF-levels were determined through bisciconitic acid method and ELISA assay, respectively. Paired t test and StataV11 software were used for statistical analysis. Results: Levels of TNF-were significantly higher in GCF from teeth with AAP than controls when standardized by either 30s of sampling and total protein content. Conclusions: The present study provides preliminary data supporting that TNF-levels in GCF reflect periapical status. Screening of TNF-levels in GCF might represent a useful sid...

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“…Among these cells, T cells are believed to play the pivotal role, which support osteoclastogenesis by producing RANKL (Kawashima, Okiji, Kosaka, & Suda, 1996;Matsuo, et al, 1992;Theill, et al, 2002), the key osteoclastogenic cytokine in osteoclast differentiation and periapical lesion formation (Yasuda, et al, 1999;Zhang & Peng, 2005). S1P, a bioactive metabolite of plasma-membrane sphingolipids, is of great importance in immune response, which regulates immune cell migration, function, polarization and cytokine production by binding with a family of G-proteincoupled receptors (S1PR1-S1P5) (Maceyka, et al, 2012;Spiegel & Milstien, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the imbalance between RANKL and OPG has been identified to play a crucial role in induced apical periodontitis of rats (Kawashima et al, 2007). Among the immune cells, activated T cells are considered to be crucial during the immune process of apical periodontitis, not only for their role in maintaining a balanced immune defence against oral bacteria, but also for their ability to produce RANKL under inflammatory circumstances and thereby inducing osteoclastogenesis (Matsuo, Ebisu, Shimabukuro, Ohtake, & Okada, 1992;Theill, et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Dissecting the Role of Sphingosine 1-Phosphate - Sphingosine 1-Phosphate Receptor 1 in Inflammatory Bone Remodelling

Xiao¹

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KeywordsOsteoimmunology, bone remodelling, osteoclastogenesis, osteogenesis, sphingosine-1-phosphate (S1P), sphingosine-1-phosphate receptor 1 (S1PR1), receptor activator of nuclear factor factor-kappa B ligand (RANKL), macrophages, mesenchymal stromal cells (MSCs), osteogenic differentiation, infection, inflammation, bone loss, apical periodontitis. Sphingosine-1-phosphate (S1P), together with its receptor, sphingosine-1-phosphate receptor 1 (S1PR1), acts as a regulator of both the immune response and bone remodelling processes and, therefore, plays a vital role in osteoimmunology.

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Quantitative analysis of immunocompetent cells in human periapical lesions: Correlations with clinical findings of the involved teeth

Cited by 48 publications

References 15 publications

Periodontal study of β-defensin 4 immunolocalization in the epithelium of human radicular cysts

Periodontal study of β-defensin 4 immunolocalization in the epithelium of human radicular cysts

Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática

Dissecting the Role of Sphingosine 1-Phosphate - Sphingosine 1-Phosphate Receptor 1 in Inflammatory Bone Remodelling

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