Quantitative Analysis of Intestinal Flora of Uygur and Han Ethnic Chinese Patients with Ulcerative Colitis

Abstract

Aim. To study the correlation between intestinal flora and ulcerative colitis by analyzing the abundance of Bacteroides, Fusobacterium, Clostridium, Bifidobacterium spp., and Faecalibacterium prausnitzii in the intestinal of ulcerative colitis (UC) patients and healthy controls with Uygur and Han ethnic. Methods. Bacterial genomic DNA was extracted from fecal samples and analyzed with real-time fluorescence quantitative polymerase chain reaction (PCR) to identify the abundance of Bacteroides, Fusobacterium, Clostridium, Bifidobacterium spp., and Faecalibacterium prausnitzii. Results. The samples from UC patients, Uygur and Han ethnic combined, had higher abundance of Bacteroides (P = 0.026) but lower Clostridium (P = 0.004), Bifidobacterium spp. (P = 0.009), and Faecalibacterium prausnitzii (P = 0.008) than those from healthy controls. Among UC patients, Bacteroides population was raised in acute UC patients (P ≤ 0.05), while the abundance of Clostridium, Bifidobacterium spp., Fusobacterium, and Faecalibacterium prausnitzii decreased (P ≤ 0.05) compared with the remission. In both UC patients group and control group, no difference was observed in the abundance of these 5 bacteria between the Han and the Uygur group. Conclusions. Variations in the abundance of these five bacterial strains in intestines may be associated with the occurrence of UC in Uygur and Han populations; however, these variations were not associated with ethnic difference.

Association analysis of dietary habits with gut microbiota of a native Chinese community

“…Several studies have used high-throughput techniques to study the mucosa-associated microbiota in IBD and IBS patients ( 54 , 55 ). Interestingly, there is still no consensus on the changes in Bacteroides abundance in colonic mucosal tissues of intestinal-related diseases ( 56 – 58 ). However, we believe that the decrease of Bacteroides on the mucosal layer due to a HFD may be involved in the perturbation of intestinal mucosal barrier, thereby promoting the occurrence of IBD or CRC.…”

Influence of Microbiota on Intestinal Immune System in Ulcerative Colitis and Its Intervention

“…In recent studies, F. prausnitzii has been demonstrated to involve in the maintenance of intestinal health ( 26 , 29 – 46 ). Of note, some studies have shown that there is a significant difference in F. prausnitzii between healthy people and UC patients ( 30 , 31 , 33 – 38 , 41 , 46 ). Compared with healthy people, F. prausnitzii species had lower counts in UC patients ( 30 – 35 , 38 , 40 – 44 ).…”

“…Of note, some studies have shown that there is a significant difference in F. prausnitzii between healthy people and UC patients ( 30 , 31 , 33 – 38 , 41 , 46 ). Compared with healthy people, F. prausnitzii species had lower counts in UC patients ( 30 – 35 , 38 , 40 – 44 ). For instance, Machiels et al introduced real-time PCR analysis to find that F. prausnitzii in UC patients had a lower abundance than health people.…”

“…Fusobacterium species are anaerobic Gram-negative bacteria, which are members of the normal microbiota of the oral and intestinal tract. However, certain species of Fusobacterium , such as adherent, invasive, pro-inflammatory species, were identified as UC pathogens ( 38 , 112 , 113 ). The relative abundance of Fusobacterium in the colonic mucosa of mouse colitis models or IBD patients is increased, among which 69% of all IBD patients-derived Fusobacterium were identified as Fusobacterium nucleatum ( 114 – 116 ).…”

Probiotics combined with aminosalicylic acid affiliates remission of ulcerative colitis: a meta-analysis of randomized controlled trial

“…The treatment of UC is mainly anti-inflammation and immune response regulation [6]. In recent years, studies have found that severe intestinal flora imbalances are closely related to the occurrence of UC, so the research and application of microecological agents are getting more and more attention [7–9]. Accumulating evidences have suggested that intestinal microbiota plays an important role in the pathogenesis of this disorder [10,11].…”