Quantitative analysis of mice’s purkinje cells under the use by anabolic steroids

Ribeiro, Cínthia Magalhães; Silva, Dauanda; Damião, Bruno; Alves, Débora Mantoan; Freitas, Ariane Cristine de; Rossi-Júnior, Wagner; Esteves, Alessandra

doi:10.4181/rnc.2014.22.03.969.6p

Cited by 3 publications

(2 citation statements)

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“…The treatment consisted in application of two anabolic steroids, the first, nandrolone decanoate marketed under the name Deca Durabolin® and the second an association of testosterone propionate, testosterone fenpropionate, testosterone isocaproate and testosterone decanoate marketed under the name of Durateston ®. The animals were treated for one (1) month, the doses of 0.02 ml physiological solution being that of Durateston® 83.3 mg / kg (0.02 ml) and the doses of Deca Durabolin® 16.6 mg / kg (0.02 ml) applied once a week [14][15][16][17][18].…”

Section: Methodsmentioning

confidence: 99%

Anabolic Steroids Effects on Bone Regeneration

Pansieri¹,

Esteves²,

Rossi³

2019

AJSS

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In recent decades, the importance given to body appearance has grown dramatically, as has the consumption of socalled "body image drugs," which include androgenic anabolic steroids (AAS), synthetic substances derived from testosterone. Although the therapeutic use of anabolic steroids are beneficial and necessary to body when there is reduction or depletion of these substances, the excessive use is related to negative interference in fracture healing process. Thus, the objective of study was to verify if there is influence of AAS in process of bone repair. For this, twenty Wistar rats were used, in which a fracture with bone loss was performed on left fibular diaphysis. Subsequently, they were divided into four groups and the animals were treated for one (1) month, with physiological solution (0.02 ml), Durateston® (83.3 mg / kg [0.02 ml]) and Deca Durabolin® (16.6 mg / kg [0.02 ml]), applied once a week. The results showed that there was a significant loss in bone healing on groups treated with AAS, since there was no regeneration in fractured fibula, besides increasing resorption process at the ends of fracture stumps. For this reason it is possible to conclude that the misuse of AAS interferes negatively in metabolism of bone tissue reinforcing the danger of indiscriminate use for health, warning that, addition other biological tissues, the bone may be affected by abuse of these substances.

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Section: Methodsmentioning

confidence: 99%

Anabolic Steroids Effects on Bone Regeneration

Pansieri¹,

Esteves²,

Rossi³

2019

AJSS

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“…From each brain so obtained, serial and homotypic samples were taken in frontal sections 13,14 with a thickness of 7 μm in a Yidi YD-315 microtome. To evaluate the areas selected for the study, the material was stained with Cresyl violet.…”

Section: The Treatment Consisted Of Intraperitoneal (Ip) Injectionmentioning

confidence: 99%

Effects of Supraphysiological Doses of Testosterone Cypionate and Stanozolol on Neuronal Density of Basolateral and Medial Amygdala and on the Anxious Behavior of Mice

et al. 2019

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Supraphysiological doses of anabolic-androgenic steroids (AAS) have been associated to possible nerve tissue damage and behavioral effects. Considering the lack of knowledge of the neural aspects involved in these behavioral alterations, this work aimed to analyze the anxious behavior response or aggressiveness, besides quantifying the neuronal density of the basolateral amygdala (BLA) and of the ventral posterior nucleus (VPN) of the medial amygdala. The animals received doses of testosterone cypionate (CT Group) and stanozolol (ST Group) twice a week for 33 days. The effects of chronic administration of AAS related to anxious behavior (as determined by the elevated plus maze [EPM] test) in male animals are observed in a lower number of entries (< 45.25%), and a shorter staying time spent in the open arms (< 41.9%) was observed in the ST. In female animals, a longer staying time spent in the closed arms of the EPM test was observed in the CT (> 15%) as well as a shorter staying time spent in the open arms for CT (< 17.1%) also to ST (< 52.1%). Regarding the neuronal density in BLA, a significant reduction in neuronal density of male animals (17.55%) was observed only in CT, whereas for females, significant differences were found in CT (19.16%) and ST (18.36%). The reduction of neuronal density in VPN in male animals was 13.55% in CT and 17.68% in ST, whereas in group of females it was 13.53% and 14.32%, respectively for CT and ST. Therefore, the two steroids used in this experiment were able to significantly reduce neuronal density in two analyzed areas, regardless of sex, suggesting that indiscriminate use of these substances causes death of brain amygdala neurons.

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Efeito de doses supra fisiológicas de esteroides anabolizantes androgênicos no cerebelo de camundongos

et al. 2015

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Introdução. Os esteroides anabólicos androgênicos (EAAs) são um grupo de compostos naturais e sintéticos formados pelo hormônio testosterona e seus derivados. A finalidade do presente estudo é analisar a densidade de células nervosas do cerebelo de camundongos. Método. Os camundongos machos, foram divididos em 4 grupos: G1: Controle (N=10), G2: Deca Durabolin® (N=10), G3: Durateston® (N=10) e G4: tratado com os dois anabolizantes (N=10), simultaneamente. Os camundongos foram tratados durante dois meses, recebendo as doses dos EAA e submetidos à natação três vezes na semana. Após a eutanásia dos animais, os cerebelos foram retirados, fixados e processados histologicamente. Os cortes foram tirados com 7μm e corados com violeta cresil e analisados por um microscópio acoplado a uma câmera e um software de análise para contagem dos corpos de neurônios. Resultados. Foram observadas diferenças nos resultados entre os animais do grupo controle (8,41) com os grupos que foram tratados com esteroides Deca Durabolin® (5,10≅39%), Durateston® (5,60≅33%) e com o grupo tratado com ambos anabolizantes (5,22≅38%). Conclusões. Desta forma, os resultados obtidos permitem concluir que houve uma diminuição, média dos grupos tratados com EAAs foi de 37%, na densidade de corpos de neurônios no córtex cerebelar.

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Quantitative analysis of mice’s purkinje cells under the use by anabolic steroids

Cited by 3 publications

References 0 publications

Anabolic Steroids Effects on Bone Regeneration

Anabolic Steroids Effects on Bone Regeneration

Effects of Supraphysiological Doses of Testosterone Cypionate and Stanozolol on Neuronal Density of Basolateral and Medial Amygdala and on the Anxious Behavior of Mice

Efeito de doses supra fisiológicas de esteroides anabolizantes androgênicos no cerebelo de camundongos

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