2008
DOI: 10.1002/jbm.a.32130
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Quantitative analysis of osteoblast‐like cells (MG63) morphology on nanogrooved substrata with various groove and ridge dimensions

Abstract: Nanotextured silicon substrata with parallel ridges separated by grooves with equal width from 90 to 500 nm, were fabricated by electron beam lithography and dry etching techniques. Osteoblast-like cells, MG-63, were cultured on the sterilized nanopatterned substrata for 4 or 24 h, and then imaged by scanning electron microscopy. The influence of substrate topography on cell morphology was analyzed by image software. We found the initially cells spread faster on the nanopatterned surfaces than on the flat surf… Show more

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Cited by 73 publications
(46 citation statements)
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“…Although by nature cells in culture are elongated (i.e., EF of 1.5 of cells on a smooth control), when cultured on grooves elongation is increased in conjunction to the alignment. In line with several other studies, depending on groove width, depth and spacing cells became highly elongated and aligned into the groove direction (Teixeira et al, 2003;Yang et al, 2008;Liliensiek et al, 2010). The alignment of cells to grooves is possibly dependent on the tyrosine phosphorylation of actin binding proteins as previously shown by Wójciak-Stothard et al (1996).…”
Section: Discussionsupporting
confidence: 90%
“…Although by nature cells in culture are elongated (i.e., EF of 1.5 of cells on a smooth control), when cultured on grooves elongation is increased in conjunction to the alignment. In line with several other studies, depending on groove width, depth and spacing cells became highly elongated and aligned into the groove direction (Teixeira et al, 2003;Yang et al, 2008;Liliensiek et al, 2010). The alignment of cells to grooves is possibly dependent on the tyrosine phosphorylation of actin binding proteins as previously shown by Wójciak-Stothard et al (1996).…”
Section: Discussionsupporting
confidence: 90%
“…Thus the actin formation reflected the underlying surface structure well, with adaptation to the micro-dimension of the pillar structure. Such a "mimicry" of the actin has also been found in other studies employing machined surfaces with groove structures [24,56,57]. The protein adsorption seems not to be the reason for the accumulation of actin on the top of the pillars because in serum-free culture conditions we observed the same phenomenon (unpublished results).…”
Section: Expression Of Integrin Receptorssupporting
confidence: 75%
“…For fibroblasts, cell spreading on 160 nm nanocolumns is reduced by 50% in comparison with the planar control [29]. For the osteoblast-like cells MG63, it could be shown that nanostructures down to 90 nm may limit lateral expansion of the cells [30]. In our experiment addressing focal adhesion capacities, the smooth surfaces (Ti-m, TZP-A-m, ATZ-m, PE) showed flattened cells with a filigree network of actin-filaments, whereas cells attached to the rough surfaces (TiUnite Ò , ZiUnite Ò , ATZ-mod) were still mainly rounded, with diffuse or limited establishment of actin-filaments.…”
Section: Discussionmentioning
confidence: 98%