Quantitative analysis of rutin content using silkworm genetic resources

Abstract

Rutin is an important bioflavonoid that is consumed in the daily diet. This study compared the functional components of rutin from various silkworm species using a gene database with those of rutin produced by silkworms selectively bred through cross-combinations. We made comparisons between the geographical origin and species of silkworm using a gene database and discovered that rutin activity was ranked in the following order by species, Chinese (C5)> miscellaneous varieties (Jamsaeng 1 Ho) >Japanese (Jam 115) > European (E58) >Korean (Sun 3 ho). However, rutin levels with respect to various genetic traits (blood color, silk color, and egg color) were consistent. In order to study rutin changes that occurred during the cross breeding of the silkworm gene, we bred cross-combinations utilizing Jam 115 and the 4051 silkworms. In conclusion, in order to provide information about the constituents of functional materials contained in silkworm powder, it is imperative that silkworm cross breeding occurs so that the database of functional materials extracted from silkworms will expand. IntroductionFlavonoids exhibit a wide variety of biological activities, including antiviral, antibacterial, anti-inflammatory, and antioxidant actions (Javed et al., 2012;Richetti et al., 2011;Nassiri-Asl et al., 2010). Among flavonoids, rutin (2-phenyl-3,5,7,3',4'-pentahydroxybenzopyrone), is a non-toxic flavonoid glycoside with P vitamin activity and a bioflavonoid and antioxidant (Schwedhelm et al., 2003;Janbaz et al., 2002). Rutin can be broadly extracted from vegetables, fruits, herbs, leaves, seeds, red wine and several plantssuch as buckwheat, passion flower, apple and tea (Harborne, 1986;Havsteen, 1983). It has