Quantitative Analysisof Stevioside and Rebaudioside a in Steviarebaudiana Leaves Using Infrared Spectroscopy and Multivariate Calibration

Martono, Yohanes; Rohman, Abdul

doi:10.22159/ijap.2019v11i1.28029

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…The 96% ethanol extract of white and red radish had a residual solvent content of 0.77% and 0.62%, respectively. The examination results correlated with the requirements for the maximum residual solvent limit in the extract, namely ≤ 1% [14].…”

Section: Characterization Of Powder and Extract Qualitysupporting

confidence: 53%

Anti-Choloseterol Activities of White (Raphanus Raphanistrum) and Red (Raphanus Sativus) Radish Roots

2021

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Objective: The purpose of this research is to explore the in vitro activities of white (Raphanus raphanistrum) and red (Raphanus sativus) radish root ethanol extract in decreasing cholesterol levels. Methods: Ultrasonification method was used in obtaining 96% ethanol extract of white and red radish root. The cholesterol levels were analyzed by visible spectrophotometry, which was validated using Lieberman-Buchard reagents. The decreased cholesterol levels were converted into IC50 values. Results: The results showed that the IC50 of 96% white and red radish root ethanol extracts were 743.7 and 634.7 µg/ml, respectively. The results of statistical analysis using the T-test obtained a significant value greater than the probability value (P) of 0.05. Conclusion: Therefore, it was concluded that the activities of 96% ethanol extract of white and red radish roots did not have a significant ability to reduce the in vitro cholesterol levels.

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Section: Characterization Of Powder and Extract Qualitysupporting

confidence: 53%

Anti-Choloseterol Activities of White (Raphanus Raphanistrum) and Red (Raphanus Sativus) Radish Roots

2021

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“…The FTIR spectrum (Figure A) of Reb A clearly shows that there are typical absorption peaks of the O–H stretching vibration at 3430 cm –1 , C–H stretching vibration at 2950 cm –1 , CO stretching vibration at 1730 cm –1 , CC stretching vibration at 1660 cm –1 , C–H scissoring and bending vibration at 1460 and 1380 cm –1 , respectively, and C–O stretching vibration at 1240 and 1080 cm –1 . After self-aggregation, an obvious blue shift of the peak from 3430 to 3400 cm –1 is observed (corresponding to the O–H stretching vibration) with a decreased adsorption intensity, while other typical peaks do not change significantly.…”

Section: Resultsmentioning

confidence: 99%

Hierarchical Self-Aggregation of Multifunctional Steviol Glycosides in Aqueous Solutions

Yang,

Liu,

et al. 2024

J. Agric. Food Chem.

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Steviol glycosides (SGs) are a natural sweetener widely used in the food and beverage industry, but the low solubility and stability of SG aqueous solutions greatly limit their application performance, especially in liquid formulations. In this work, we explore the solubility behavior of rebaudioside A (Reb A) in water, a major component of SGs, with the aim of clarifying the underlying mechanisms of the solubility and stability constraints of SGs, as well as the impact on their multifunctional properties. We demonstrate for the first time that Reb A exhibits hierarchical self-assembly in solutions, forming spherical micelles first when the concentration exceeds its critical micelle concentration (5.071 mg/mL), which then further assemble into large rod-like aggregates. The formation of such large Reb A aggregates is mainly dominated by hydrogen bonding and short-range Coulomb interaction energy, thus leading to the low solubility and precipitation of Reb A solutions. Surprisingly, aggregated Reb A structures display significantly improved organoleptic properties, revealing that self-aggregation can be developed as a simple, efficient, and green strategy for improving the taste profile of SGs. Additionally, the self-aggregation of Reb A at high concentrations impairs active encapsulation and also affects its interfacial and emulsifying properties.

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“…The scanning of FTIR spectra of Sidaguri powder was carried out using an FTIR spectrophotometer (Thermo Scientific Nicolet iS10, Madison, WI), controlled with the software Omnic, according to Martono et al [16]. The measurements were carried in the mid-infrared region of 4000-650 cm -1 with 32 scannings with a resolution of 8 cm -1 using horizontal attenuated total reflectance (HATR) composed of ZnSe crystal.…”

Section: Ftir Spectra Measurementmentioning

confidence: 99%

The Use of Chemometrics for Classification of Sidaguri (<i>Sida rhombifolia</i>) Based on FTIR Spectra and Antiradical Activities

et al. 2021

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Sidaguri (Sida rhombifolia) is one of the herbal components used in traditional medicine. The application of chemometrics in the standardization of herbal medicine is common. The objective of this study was to classify Sidaguri from different regions based on FTIR spectra with chemometrics of principal component analysis (PCA) and to correlate the antioxidant activities with FTIR spectra using the multivariate calibration of partial least square regression (PLSR). The extraction of Sidaguri powder was performed using ultrasound-assisted extraction (UAE) at optimum conditions. The obtained extracts were subjected to antiradical scavenging activities using DPPH (2,2’-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) radicals. The PCA result shows that Sidaguri from different regions could be separated using 14 wavenumbers of FTIR spectra based on the PCA's loading plot. PLSR regression using the second derivative FTIR spectra at wavenumbers of 3662–659 cm–1 could predict radical scavenging activities (RSA) of Sidaguri with R2 values of 0.9636 and 0.9024 for calibration and validation models, with RMSEC and RMSEP values of 1.45% and 2.65%, respectively. It can be concluded that FTIR spectra treated by PCA were reliable for classifying Sidaguri from different regions. At the same time, PLSR was accurate and precise enough to predict the RSA of Sidaguri.

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Quantitative Analysisof Stevioside and Rebaudioside a in Steviarebaudiana Leaves Using Infrared Spectroscopy and Multivariate Calibration

Cited by 9 publications

References 24 publications

Anti-Choloseterol Activities of White (Raphanus Raphanistrum) and Red (Raphanus Sativus) Radish Roots

Anti-Choloseterol Activities of White (Raphanus Raphanistrum) and Red (Raphanus Sativus) Radish Roots

Hierarchical Self-Aggregation of Multifunctional Steviol Glycosides in Aqueous Solutions

The Use of Chemometrics for Classification of Sidaguri (<i>Sida rhombifolia</i>) Based on FTIR Spectra and Antiradical Activities

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