Quantitative and cytological studies of interstitial (Leydig) cells in the scrotal and retained testes of unilateral cryptorchid West African Dwarf goats

Okpe, Godwin Chidozie; Ezeasor, D. N.

doi:10.1016/j.smallrumres.2015.11.007

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…The findings of the present study showed that goat Leydig cells possess the general cytological features characteristic of mammalian Leydig cells (Okpe & Ezeasor, 2016). The interdigitating finger-like processes and the junctional complexes resemble those already described by earlier workers (Connell & Christensen, 1975;Ezeasor, 1985;Tong et al, 2015).…”

Section: Discussionsupporting

confidence: 88%

The morphological postnatal development of the testis of the Nubian bucks

Shaaeldin

Tingari

Kumar

et al. 2022

Anat Histol Embryol

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Goat population in the Sudan is estimated to be around 40 million heads. Nubian goats constitute about 60% of this goat population.They played an important economical role in the livelihood of a large sector of the population in the rural areas. Nubian goats are well reputed as one of the prominent breeds of dairy goats in the tropics (Devendra & McLeroy, 1982;Hassan & El-Derani, 1990;Sulieman & El Shafei, 1984). Nubian goats are the progenitors of the world famous Anglo-Nubian breed (Aboul-Naga et al., 2012;Williamson & Payne, 1978). Goats are used as a representative of large animals for certain experimental purposes like studies on reproduction as the reproductive tracts of the male goat, ram and bull are essentially similar (Goyal, 1985). Among these three species, male goats receive preference because, unlike rams, and bulls, are inexpensive and easier to handle for surgical manoeuvres

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Section: Discussionsupporting

confidence: 88%

The morphological postnatal development of the testis of the Nubian bucks

Shaaeldin

Tingari

Kumar

et al. 2022

Anat Histol Embryol

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“…There are significant differences in the composition of testicular interstitial tissues among different animal species, such as cell composition, the proportion of connective tissue, the degree of development of lymphatic vasculature and the location of blood vessels (Azevedo et al., 2008). Okpe and Ezeasor (2016) reported that cryptorchid testes of West African Dwarf goats had looser connective tissues of the seminiferous tubules due to underdevelopment. Research suggests that the basement membranes thickness of seminiferous tubule wall apparently decreases with ageing, whereas lamina propria presents extremely variable degenerative changes (Pop et al., 2011).…”

Section: Discussionmentioning

confidence: 99%

INSL‐3 protein expression in normal and cryptorchid testes of Ziwuling black goats

Yuan

Wang

et al. 2021

Reprod Domestic Animals

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Ziwuling black goats are typically found in loess plateaus regions and the Ziwuling Nature Reserve. Cryptorchidism is a common disease in this inbred goat, and its pathogenesis has been linked with the expression of insulin‐like factor 3 (INSL‐3). Therefore, this study aimed to investigate anatomical alterations caused by cryptorchism and the expression and distribution of INSL‐3 in normal and cryptorchid testicular tissues. The testicular tissues of 6‐month‐old Ziwuling black goats were collected for microscopic analyses using histochemical, immunohistochemical, immunofluorescence and biometrical methods, as well as Western blotting to compare the expression and distribution of INSL‐3. A lower expression of INSL‐3 was observed in cryptorchid compared with normal testicular tissues (p < .01). Cryptorchidism caused a significant reduction in layers of spermatogenic epithelium and tubule areas in Ziwuling black goat (p < .01). The interstitial to seminiferous tubule area ratio was larger in cryptorchid than in normal group. Periodic Acid‐Schiff (PAS) staining revealed pronounced positive bands in the interstitial tissue, while positive Alcian blue (AB) staining was not clear, and AB‐PAS staining revealed a positive red band in the basement membrane of cryptorchid group. Immunofluorescence revealed a strong signal of INSL‐3 expression in Sertoli and peritubular myoid cells, and moderate signal in Leydig and spermatogenic cells in the normal group. However, in cryptorchid testicular tissues, the signal of INSL‐3 expression was strong in primary spermatocytes, occasional in Sertoli cells, limited in Leydig cells and absent in peritubular myoid cells. Furthermore, immunohistochemistry showed that INSL‐3 expression was higher in normal testes compared with cryptorchid testicular tissues (p < .05), especially in primary spermatocytes and Sertoli cells. Collectively, our results indicate that cryptorchidism is closely related to the disorder of acid glycoprotein metabolism and the reduction in release of INSL‐3 from Leydig cells. Moreover, Sertoli and peritubular myoid cells are crucial for INSL signalling and could underpin further research on the mechanism of cryptorchidism in animal.

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“…In addition, a notable study on bucks with natural unilateral cryptorchidism further confirmed how the lengths, heights, and diameters of the seminiferous tubules in the contralateral testes are significantly higher than the retained testes, and that the percentage of spermatogenic cells per testis did not differ from those calculated in normal control bucks. Additionally, higher volumes of Leydig cells and seminiferous tubules in the contralateral scrotal testes were observed compared to the bucks with normal testes, indicating that the spermatogenic efficiency of the scrotal testes of bucks with unilaterally cryptorchid testes was higher than that of the control bucks [ 48 ]. These data further support our hypothesis that the contralateral scrotal testis in cases of unilateral cryptorchidism retains its reproductive potential.…”

Section: Discussionmentioning

confidence: 99%

Stereological study of testes following experimentally-induced unilateral cryptorchidism in rats

Sadeghinezhad,

Yarmahmoudi,

Dehghan

et al. 2023

Clin Exp Reprod Med

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Objective: Cryptorchidism is one of the main causes of infertility and can result in testicular cancer. This study aimed to present quantitative data on the damage caused by cryptorchidism using stereological analysis.Methods: Thirty newborn rats were randomly divided into control and experimental groups. The experimental group underwent surgery to induce unilateral cryptorchidism in the left testis, whereas the control group underwent a sham surgical procedure 18 days after birth. The testes were removed at designated time points (40, 63, and 90 days after birth) for stereological evaluation and sperm analysis. Total testicular volume, interstitial tissue volume, seminiferous tubule volume and length, and seminiferous epithelium volume and surface area were measured. Other parameters, such as sperm count, sperm morphology, and sperm tail length, were also examined. Results: Statistically significant differences (<i>p</i><0.05) were observed between the experimental and the control groups at different ages regarding the volumes of various parameters, including the surface area of the germinal layer, the length of the seminiferous tubules, sperm count, and sperm morphology. However, no significant differences were observed in the epithelial volume and the sperm tail length of the groups. Conclusion: Given the substantial effect of cryptorchidism on different testicular parameters, as well as the irreversible damage it causes in the testes, it is important to take this abnormality seriously to prevent these consequences.

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Quantitative and cytological studies of interstitial (Leydig) cells in the scrotal and retained testes of unilateral cryptorchid West African Dwarf goats

Cited by 4 publications

References 30 publications

The morphological postnatal development of the testis of the Nubian bucks

The morphological postnatal development of the testis of the Nubian bucks

INSL‐3 protein expression in normal and cryptorchid testes of Ziwuling black goats

Stereological study of testes following experimentally-induced unilateral cryptorchidism in rats

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