Quantitative assay of lactate dehydrogenase isoenzymes by reflectance densitometry

Latner, A. L.; Turner, D.M.

doi:10.1016/0009-8981(67)90330-0

Cited by 14 publications

(4 citation statements)

References 7 publications

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“…The percentage M sub-unit contribution to the LDH isoenzyme patterns of the cultured explants was estimated quantitatively following vertical starch gel electrophoresis by reflectance densitometry according to the method of Latner and Turner (1967).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Malignant Cell Invasion in vitro by a Proteinase Inhibitor

Latner¹,

Longstaff²,

Pradhan³

1973

Br J Cancer

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Summary.-The inhibitory effect of the protease inhibitor aprotinin (Trasylol) on the invasion of mouse kidney explants by polyoma virus transformed BHK21 cells was investigated using a mixed cell/organ culture technique. The extent of invasion was monitored by following the changes in LDH isoenzyme pattern in the explants and by histological assessment. The kidney explants containing aprotinin were found to maintain a normal kidney LDH pattern and to suffer considerably less invasion than the explants not containing the drug. These results support the idea that proteolytic enzymes are associated with invasion and that inhibitors of protease activity could possibly be useful in the management of clinical cancer.

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Section: Methodsmentioning

confidence: 99%

Inhibition of Malignant Cell Invasion in vitro by a Proteinase Inhibitor

Latner¹,

Longstaff²,

Pradhan³

1973

Br J Cancer

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“…Lactate dehydrogenase isoenzymes were estimated by reflectance densitometry (Latner and Turner, 1967) after separation by starch gel electrophoresis (Smithies, 1959) in Tris-borate-EDTA buffers, pH 8.6, (Boyer et ul, 1963) and staining by a method essentially that of Latner and Skillen (1961). The proportions of the two subunits were calculated from the known tetrameric structure of the five types of isoenzyme molecule present in most tissues (Shaw and Barto, 1963).…”

Section: Enzyme Assaymentioning

confidence: 99%

The Effect of the Menopause on the L‐lactate‐nicotinamide‐adenine Dinucleotide Oxidoreductase Activity in Vaginal Aspirates

Emes

Latner

Guthrie

et al. 1980

BJOG

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Summary Lactate dehydrogenase (L‐Iactate‐NAD+ oxidoreductase; EC 1.1.1.27) activity has been assayed in saline extracts of vaginal aspirates obtained from patients attending a Well Woman Clinic and for whom exfoliative cytology was shown to be unequivocally normal. The majority (76 per cent) of premenopausal patients gave aspirate extracts containing less than 25 m units of enzyme while 71 per cent of postmenopausal patients gave aspirate extracts containing more than 50 m units of enzyme. This difference in distribution of activities was highly significant (P = <0.001). Analysis of the isoenzyme distribution showed that aspirates from postmenopausal patients contained a lower proportion of isoenzyme LDH‐5 (P <0.01) and the M subunit (P <0.03) and a higher proportion of isoenzymes LDH‐3 (P <0.001) and LDH‐4 (P <0.01). There was no significant difference between the social class distributions of the pre‐ and postmenopausal patients. Lactate dehydrogenase activity was not related to social class or patients' multiparity. No difference in the distribution of enzyme activities was found between those premenopausal patients in the proliferative phase of the menstrual cycle, those in the secretory phase and those receiving hormone therapy.

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“…The resolution power in starch is higher than that in agarose [27,53,54,55], but the quantitative evaluation of isoenzyme activities, when performed by reflectance densitometry, is more readily performed in agarose than in starch gels. Moreover, the results obtained from agarose are easier to reproduce than those from starch gels, provided that the recommendations given by Latner and T urner [29] are followed. In some analyses in the present investigation, however, too intense colour was obtained in the LDj to give a relevant measure of the activity of the corresponding isoenzyme.…”

Section: Demonstration Of the Isoenzymes Of Ldh And Mdh By Zone Electmentioning

confidence: 99%

Lactic and Malic Dehydrogenase Activities and Isoenzyme Patterns in Blood Sera of Developing Neonatal Piglets

Karlsson¹

1968

Neonatology

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Quantitative assay of lactate dehydrogenase isoenzymes by reflectance densitometry

Cited by 14 publications

References 7 publications

Inhibition of Malignant Cell Invasion in vitro by a Proteinase Inhibitor

Inhibition of Malignant Cell Invasion in vitro by a Proteinase Inhibitor

The Effect of the Menopause on the L‐lactate‐nicotinamide‐adenine Dinucleotide Oxidoreductase Activity in Vaginal Aspirates

Lactic and Malic Dehydrogenase Activities and Isoenzyme Patterns in Blood Sera of Developing Neonatal Piglets

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