Quantitative assessment of bladder carcinoma by acid labile DNA assay

Abstract

BACKGROUND Effective noninvasive methods for monitoring patients with bladder carcinoma and screening for bladder carcinoma that show better performance than the methods currently in use would be desirable for detecting urothelial carcinoma at an early, easily treatable stage. A rapidly hydrolyzed component of nuclear DNA has been described, the increase of which has been linked to malignancy. Quantitative determination of acid labile DNA has been applied successfully to detect other neoplasms. This study investigates the potential of this method to detect transitional cell carcinomas. METHODS Touch imprints of transurethral resection material from 62 cases of nonmalignant urothelium (control group including reactive changes) and 94 cases of bladder carcinoma were analyzed. The full Feulgen hydrolysis profiles of the nonmalignant and malignant urothelial cells were compared by measuring the staining density of the nuclei using digital image analysis after various hydrolysis times. Twenty cells were sampled randomly from among the cells measured for calculation of the mean optical density (MOD). The MOD of each time was used to build the hydrolysis profile of a case. RESULTS A hydrolysis time of 10 minutes was found to be the most discriminative between control and carcinoma cases. Applying a single threshold MOD value of 101 resulted in a test sensitivity of 95.7%, a specificity of 94.4%, a positive predictive value of 98.3%, a negative predictive value of 95.9%, and an area under the receiver operating characteristic curve of 0.97. CONCLUSIONS The results of this pilot study suggest that measurement of the rapidly hydrolyzed component of DNA present in the nuclei of bladder urothelium offers a highly sensitive and reliable supplement to the qualitative and subjective cytologic procedures currently in use. Cancer 1999;86:105–13. © 1999 American Cancer Society.

Fluorescence in-situ hybridisation on biopsies from clam ileocystoplasties and on a clam cancer

“…Polysomy is known to be an unfavourable prognostic marker and is associated with rapid progression of a cancer (Gschwendtner and Mairinger, 1999;Krause et al, 2003;Deliveliotis et al, 2005). Therefore, the presence of polysomy within this fatal squamous cell carcinoma was consistent with the aggressive nature of this tumour.…”

Nuclear features of infiltrating urothelial carcinoma are distinguished from low-grade noninvasive papillary urothelial carcinoma by image analysis

“…The present study utilized WSI and IA to clarify nuclear features from urothelial carcinoma tissue samples as nuclear morphological analysis of urothelial carcinoma had not been previously performed in detail. However, certain studies have performed cell size analysis through digital imaging in bladder tissue samples ( 25 ), morphometric detection of urothelial cancer cell nests using an algorithm ( 5 ) and quantitative assessment of bladder carcinoma using an acid labile DNA assay ( 26 ). The results of the present study revealed that the SD of nuclear maximum and minimum diameter, SD of nuclear area (size), SD of nuclear average density, SD of nuclear maximum density, nuclear average density and nuclear maximum density were significantly higher in IUC compared with in LGPUC samples.…”