Quantitative counter-immunoelectrophoresis for estimation of antibodies to infectious bursal disease virus

Abstract

Quantitative counter-immunoelectrophoresis was standardized to detect antibodies to the avian infectious bursal disease virus. This technique correlated well with the conventional quantitative agar gel precipitation test in estimating antibodies to IBDV. The use of blood dried on filter paper as an alternative to serum is discussed. QCIE is simple, easy to perform and faster than QAGP.

Changes in the Cytokine and Toll-Like Receptor Gene Expression Following Infection of Indigenous and Commercial Chickens With Infectious bursal disease virus

“…Indigenous (Aseel type) chickens were obtained from individual households from remote villages of Tamil Nadu whose date of hatch was known. The serum from both groups of birds were examined for antibodies to IBDV using the agar gel precipitation test (AGPT) with the infective bursal suspension as IBDV antigen and reference antiserum obtained from Central Veterinary laboratory, Weybridge, United Kingdom as positive control serum as described [19]. These chickens were housed in isolation rooms at the Department of Animal Biotechnology Animal Facility, Madras Veterinary College, Chennai, India.…”