2011
DOI: 10.1371/journal.pone.0020384
|View full text |Cite
|
Sign up to set email alerts
|

Quantitative Detection and Biological Propagation of Scrapie Seeding Activity In Vitro Facilitate Use of Prions as Model Pathogens for Disinfection

Abstract: Prions are pathogens with an unusually high tolerance to inactivation and constitute a complex challenge to the re-processing of surgical instruments. On the other hand, however, they provide an informative paradigm which has been exploited successfully for the development of novel broad-range disinfectants simultaneously active also against bacteria, viruses and fungi. Here we report on the development of a methodological platform that further facilitates the use of scrapie prions as model pathogens for disin… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
76
0

Year Published

2012
2012
2023
2023

Publication Types

Select...
4
2

Relationship

2
4

Authors

Journals

citations
Cited by 46 publications
(78 citation statements)
references
References 45 publications
2
76
0
Order By: Relevance
“…24 For the preparation of glial cell cultures, normal neonatal Syrian hamsters (for strain and supplier see above) were used as donors of brain tissue and sacrificed 2-3 days after birth by decapitation. 15 Although not mandatory, we reported euthanasia of normally Syrian hamsters to the animal protection authority (Landesamt fü r Gesundheit und Soziales Berlin, Berlin, Germany; Registration Number T0220/07).…”
Section: Use Of Normal Hamsters and Micementioning
confidence: 99%
See 4 more Smart Citations
“…24 For the preparation of glial cell cultures, normal neonatal Syrian hamsters (for strain and supplier see above) were used as donors of brain tissue and sacrificed 2-3 days after birth by decapitation. 15 Although not mandatory, we reported euthanasia of normally Syrian hamsters to the animal protection authority (Landesamt fü r Gesundheit und Soziales Berlin, Berlin, Germany; Registration Number T0220/07).…”
Section: Use Of Normal Hamsters and Micementioning
confidence: 99%
“…13,14,18 When the biological infectivity and biochemical seeding activity of 263K scrapie prions in different sample materials were systematically compared, this empirically confirmed a consistent quantitative correlation between the biological infectivity and biochemical seeding activity. 14,15 Rationale for the further reduction and replacement of prion titrations in animals by quantitative cell and PMCA assays Quantitative prion bioassays in animals usually determine infectivity titres in terms of the median infective doses (ID 50 ) that had been present in the inoculated sample material. One prion ID 50 is the dose of prions that causes infection in 50% of inoculated animals.…”
Section: Biochemical Assays For the Measurement Of Prion-associated Smentioning
confidence: 99%
See 3 more Smart Citations