Quantitative Detection of Glyphosate by Simultaneous Analysis of UV Spectroscopy and Fluorescence Using DNA-Labeled Gold Nanoparticles

Abstract

A sandwich-type immunosensor composed of antigen-double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen-double DNA-gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg mL(-1) for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 μg mL(-1)), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.

The role of derivatization techniques in the analysis of glyphosate and aminomethyl-phosphonic acid by chromatography

“…For sake of completeness it is worth mentioning the DNA-labeled gold nanoparticles (NPs) suitable for the measurement of free GLYP concentration [80]. The reaction between the antigen -double DNA -gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were determined on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg/mL for the detection of immobilized antigen-double DNA-gold NPs.…”

“…In this review those relevant papers were compiled -without completeness -in which for GLYP and AMPA analysis -GC , LC , CEC [71,72], ion chromatography (IC) [73][74][75], spectrophotometry [76,77] and neutron magnetic resonance (NMR) [78][79][80] protocols were used. Further classifications were listed according to the matrix they had to be extracted from and/or to the detailed process they were subjected to, prior to their identification and quantitation analysis.…”

Nanocristais na Detecção de Glifosato

“…Unfortunately, this study did not explore the possible cross-reactions with GLY analogues and possible environmental interferents. This study further improves the previous report by the same authors, who developed a competitive inhibition assay by free GLY using GLY-dsDNA-gold conjugate nanoparticles, which was used to quantify fluorescence intensity through an immunoassay (FS-AU/DNA) (Lee et al 2010).…”

Development of magnetic luminescent core/shell nanocomplex particles with fluorescence using Rhodamine 6G

“…The stamps were blow-dried and then carefully placed on amine-terminated self-assembled monolayers (SAMs) on clean glass substrates [29]. A modified amine group on the patterned glass substrate was prepared following a standard method [30]. After patterning the amine-terminated SAMs, the substrate was rinsed copiously with distilled water and dried at 60 8C for 2 h. The substrate was then modified with 400 mL sodium phosphate buffer (0.1 M, pH 8.0) by soaking the substrate in 2.6 M glutaraldehyde at 4 8C for 1 h and then at 20 8C for 1 h. After the substrate was washed with distilled water, the micro-patterned glass substrate with the aldehyde-terminated SAMs was immersed in the prepared magnetic particle solution for 2 h. This procedure was accomplished through an imine reaction between the amine groups on the surface of the particles and the aldehyde groups on the substrate (Fig.…”