Quantitative detection of pathogen load of Fusarium oxysporum f.sp. ciceris infected wilt resistant and susceptible genotypes of chickpea using intergenic spacer region-based marker

Priyadarshini, Parichita; Kohli, D.; Yadav, Sheel; Srinivasa, N.; Bharadwaj, Chellapilla; Anjoy, Priyanka; Gaikwad, Kishor; Jain, Pradeep

doi:10.1016/j.pmpp.2021.101622

Cited by 1 publication

(1 citation statement)

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“…They have a fairly high accuracy and reproducibility, but large time and economic costs also do not make them optimal for predicting food contamination. Mycological analysis, enzyme immunoassay, real-time PCR [1,2], and quantitative digital PCR, using markers based on intergenic spacers [3], are used to diagnose fusarium lesions. The common disadvantages of these methods are extremely expensive equipment and reagents, the duration of the analysis of up to 7-10 days, and the need for highly qualified specialists to conduct them.…”

Section: Introductionmentioning

confidence: 99%

Determination of Main Spectral and Luminescent Characteristics of Winter Wheat Seeds Infected with Pathogenic Microflora

et al. 2021

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In connection with the constant growth of demand for high-quality food products, there is a need to develop effective methods for storing agricultural products, and the registration and predicting infection in the early stages. The studying of the physical properties of infected plants and seeds has fundamental importance for determining crop losses, conducting a survey of diseases, and assessing the effectiveness of their control (assessment of the resistance of crops and varieties, the effect of fungicides, etc.). Presently, photoluminescent methods for diagnosing seeds in the ultraviolet and visible ranges have not been studied. For research, seeds of winter wheat were selected, and were infected with one of the most common and dangerous diseases for plants—fusarium. The research of luminescence was carried out based on a hardware–software complex consisting of a multifunctional spectrofluorometer “Fluorat-02-Panorama”, a computer with software “Panorama Pro” installed, and an external camera for the samples under study. Spectra were obtained with a diagnostic range of winter wheat seeds of 220–400 nm. Based on the results obtained for winter wheat seeds, it is possible to further develop a method for determining the degree of fusarium infection.

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Section: Introductionmentioning

confidence: 99%