Quantitative Determination of 3D-Printing and Surface-Treatment Conditions for Direct-Printed Microfluidic Devices

Namgung, Hyun; Kaba, Abdi Mirgissa; Oh, Hyeonkyu; Jeon, Hyun-Jin; Yoon, Jeonghwan; Lee, Haseul; Kim, Dohyun

doi:10.1007/s13206-022-00048-1

Cited by 13 publications

(10 citation statements)

References 85 publications

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“…Various approaches for improving this property have been suggested in published papers as well as on vendor and hobbyist websites (r\3Dprinting, All3DP, etc.). [37][38][39] Here we compared 5 of these methods head to head, using the FL Clear resin as a base, to determine which would be best for increasing the transparency of a printed piece (Fig. 9).…”

Section: Optical Components Of Resinsmentioning

confidence: 99%

“…1). [24][25][26] For commercial resins, strategies to diminish resin cytotoxicity have started to emerge recently, [27][28][29][30] along with solutions addressing print resolution, [31][32][33][34][35][36] imaging compatibility, 33,[37][38][39] and surface modification and bonding. 40,41 Sifting through the plethora of literature for best practices can be challenging for researchers who are new to this rapidly growing field.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Musgrove

Catterton

Pompano

2022

Analytica Chimica Acta

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Section: Optical Components Of Resinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Musgrove

Catterton

Pompano

2022

Analytica Chimica Acta

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“…In addition to the channel height, we believe that other variables can also influence the effectiveness of the MNP filtration for the proposed microfluidic chip. First, the MNP concentration is crucial to the dynamic range that can be tuned to fit the clinical cutoff value [ 27 , 28 ]. It is essential to obtain enough MNPs to cluster in the reaction chamber.…”

Section: Resultsmentioning

confidence: 99%

“…The 3D printing process offers better reproducibility and a high throughput production of the microstructure for microfluidics [ 24 , 25 ]. Several methods for 3D printing techniques, such as selective laser sintering, the extrusion method, inkjet printing, and stereolithography (SLA), offer a broad spectrum in terms of cost, resolution, roughness, stiffness, and additive materials [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Labeling on a Chip of Cellular Fibronectin and Matrix Metallopeptidase-9 in Human Serum

et al. 2022

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We present a microfluidic chip for protein labeling in the human serum-based matrix. Serum is a complex sample matrix that contains a variety of proteins, and a matrix is used in many clinical tests. In this study, the device performance was tested using commercial serum samples from healthy donors spiked with the following target proteins: cellular fibronectin (c-Fn) and matrix metallopeptidase 9 (MMP9). The microfluidic molds were fabricated using micro milling on acrylic and using stereolithography (SLA) three-dimensional (3D) printing for an alternative method and comparison. A simple quality control was performed for both fabrication mold methods to inspect the channel height of the chip that plays a critical role in the labeling process. The fabricated microfluidic chip shows a good reproducibility and repeatability of the performance for the optimized channel height of 150 µm. The spiked proteins of c-Fn and MMP9 in the human serum-based matrix, were successfully labeled by the functionalized magnetic nanoparticles (MNPs). The biomarker labeling occurring in the serum was compared using a simple matrix sample: phosphate buffer. The measured signals obtained by using a magnetoresistive (MR) biochip platform showed that the labeling using the proposed microfluidic chip is in good agreement for both matrixes, i.e., the analytical performance (sensitivity) obtained with the serum, near the relevant cutoff values, is within the uncertainty of the measurements obtained with a simple and more controlled matrix: phosphate buffer. This finding is promising for stroke patient stratification where these biomarkers are found at high concentrations in the serum.

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“…The ability to print leak-free devices, small internal microchannels (<1000 μm), and biocompatible, optically-clear devices depends on several factors that will be discussed further below, including aspects that affect mechanical integrity and the rate and resolution of photopolymerization (Fig.1). 24–26 For commercial resins, strategies to diminish resin cytotoxicity have started to emerge recently, 27–30 along with solutions addressing print resolution, 31–36 imaging compatibility, 33,37–39 and surface modification and bonding. 40,41 Sifting through the plethora of literature for best practices can be challenging for researchers who are new to this rapidly growing field.…”

Section: Introductionmentioning

confidence: 99%

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Musgrove

Catterton

Pompano

2021

Preprint

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Stereolithographic (SL) 3D printing, especially digital light processing (DLP) printing, is a promising rapid fabrication method for bio-microfluidic applications such as clinical tests, lab-on-a-chip devices, and sensor integrated devices. The benefits of 3D printing lead many to believe this fabrication method will accelerate the use of bioanalytical microfluidics, but there are major obstacles to overcome to fully utilize this technology. For commercially available printing materials, this includes challenges in producing prints with the print resolution and mechanical stability required for a particular design, along with cytotoxic components within many SL resins and low optical compatibility for imaging experiments. Potential solutions to these problems are scattered throughout the literature and rarely available in head-to-head comparisons. Therefore, we present here principles for navigation of 3D printing techniques and systematic tests to inform resin selection and optimization of the design and fabrication of SL 3D printed bio-microfluidic devices.

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Quantitative Determination of 3D-Printing and Surface-Treatment Conditions for Direct-Printed Microfluidic Devices

Cited by 13 publications

References 85 publications

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Labeling on a Chip of Cellular Fibronectin and Matrix Metallopeptidase-9 in Human Serum

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

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