“…Different analytical methods have been published for the determination of GLM and PIO either alone or in combination including spectrophotometric [4][5][6][7], spectrofluorometric [8], HPLC [1,[9][10][11][12][13][14][15], TLC [1,16,17]. Several methods have been used for determination of VAL [2,[18][19][20][21][22][23][24][25][26][27], whereas ATV was determined by spectrophotometric [3,28], spectrofluorometric [29], HPLC [24,[30][31][32], TLC [33]and electrochemical [34] methods. UPLC technology takes full advantages of chromatographic principles to run separations.…”