Quantitative Determination of Chitosan and the Percentage of Free Amino Groups

Curotto, E.; Arós, Fernando

doi:10.1006/abio.1993.1263

Cited by 155 publications

(78 citation statements)

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“…The product was cooled to room temperature and dissolved in deionized water to produce a 33 wt% stock solution. Completion of the reaction was verified by the disappearance of free amine in a Ninhydrin test (Moeser et al, 2002;Curotto and Aros, 1993).…”

Section: Cell-resistant Surface Lnodificationmentioning

confidence: 97%

Microbioreactors for Bioprocess Development

Zhang

Perozziello

Boccazzi

et al. 2007

JALA: Journal of the Association for Laboratory Automation

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As a step toward high-throughput bioprocess development, we present design, fabrication, and characterization of polymer based microbioreactors integrated with automated sensors and actuators. The devices are realized, in increasing levels of complexity, in poly(dimethylsiloxane) and poly(methyl methacrylate) by micromachining and multilayer thermal compression bonding procedures. Online optical measurements for optical density, pH, and dissolved oxygen are integrated. Active mixing is made possible by a miniature magnetic stir bar. Plug-in-and-flow microfluidic connectors and fabricated polymer micro-optical lenses/connectors are integrated in the microbioreactors for fast set up and easy operation. Application examples demonstrate the feasibility of culturing microbial cells, specifically Escherichia coli, in 150 μL-volume bioreactors in batch, continuous, and fed-batch operations. (JALA 2007;12:143–51)

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Section: Cell-resistant Surface Lnodificationmentioning

confidence: 97%

Microbioreactors for Bioprocess Development

Zhang

Perozziello

Boccazzi

et al. 2007

JALA: Journal of the Association for Laboratory Automation

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show abstract

“…• conductometric titration (Raymond et al, 1993) (soluble material) • UV-VIS (Muzzarelli, 1985) (soluble material) • circular dichroism (Domard, 1987) (soluble material) • thermal analysis (Alonso et al, 1983) (solid material) • enzymatic hydrolysis (Nanjo et al, 1991) (soluble material) • picric acid assay (Neugebauer et al, 1989) (soluble material) • pyrolysis GC (Lal & Hayes,1984) (solid material) • acid hydrolysis GC (Holan et al, 1980) (solid material) • acid hydrolysis HPLC (Niola et al, 1993) (solid material) • ninhydrin test (Curotto & Aros, 1993;Prochazkova et al, 1999) (soluble material) • X-ray deffraction ) (solid material) Since chitosan is a natural product it may contain certain residues (protein, alkali, salt, astaxanthin), depending on the quality of the chitin source and the reaction conditions. Problems with sample contamination may arise with several methods.…”

Section: Da Determinationmentioning

confidence: 99%

Influence of the Chemical Structure and Physicochemical Properties of Chitin- and Chitosan-Based Materials on Their Biomedical Activity

Kumirska¹,

Weinhold²,

Czerwicka³

et al. 2011

Biomedical Engineering, Trends in Materials Science

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“…It is based on a colorimetric reaction where the ninhydrin reacts with the primary amino groups to form a colored product, diketohydrindylidene-diketohydrindamine, which can be measured at 570 nm in a spectrophotometer (Curotto & Aros, 1993;Prochazkova, Va˚rum, & Ostgaard, 1999;Wei, Cruz, & Gorski, 2002). Its main advantage is the use of a minimal amount of sample (Tan et al, 1998).…”

Section: Ninhydrin Testmentioning

confidence: 99%