Quantitative determination of doxepin and desmethyldoxepin in rat plasma by means of gas-liquid chromatography-mass fragmentography

Frigerio, A.; Pantarotto, C.; Franco, R.; Goméni, Roberto; Morselli, P. L.

doi:10.1016/s0021-9673(00)89821-0

Cited by 14 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…equipped with an all-glass chromatographic inlet system and interfaced 4 Pierce Chemical Co. 5 Hewlett-Packard 5730 A series. equipped with an all-glass chromatographic inlet system and interfaced 4 Pierce Chemical Co. 5 Hewlett-Packard 5730 A series.…”

Section: Methodsmentioning

confidence: 99%

Quantitative GLC Determination of cis- and trans-Isomers of Doxepin and Desmethyldoxepin

Rosseel¹,

Bogaert²,

Claeys

1978

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Quantitative GLC Determination of cis- and trans-Isomers of Doxepin and Desmethyldoxepin

Rosseel¹,

Bogaert²,

Claeys

1978

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

“…Depending on species, different half‐lives have been documented in the literature. For humans and rats t 1/2 was reported to be 16.8 (Ziegler et al ., 1978) and 0.9 h (Frigerio et al ., 1977), respectively. However, in these two studies doxepin isomers were not individually monitored.…”

Section: Discussionmentioning

confidence: 99%

“…The time course of the isomer concentrations can be described as linear regressions (r ±0.800 to ±0.980) with respect to the interval of 1±12 h, and from these data, b-half-lives (b-t 1/2 ) of the doxepin isomers in horse blood may be calculated as was previously demonstrated for rats (Frigerio et al, 1977). However, because of the irregular concentration maxima found in serum of animal 3 at 3 and 8 h (Figs 1 & 2), correlation of data was less successful and therefore calculation of b-t 1/2 was omitted in this case.…”

Section: Concentrations Of the Doxepin Isomers In Treated Horsesmentioning

confidence: 95%

Elimination of doxepin isomers from the horse following intravenous application

Hagedorn

Meiser

Zankl

et al. 2001

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

The tricyclic antidepressant doxepin, representing a 5:1 mixture of trans- and cis-isomers, owns tranquilizing properties. This compound has been associated with illicit medication of racing horses, and therefore should be considered in doping control. Because analysis of doxepin in equine body fluids has not been documented in the literature, a highly sensitive analytical method was developed to individually monitor the doxepin isomers in blood and urine of horses by the use of gas chromatography/mass spectrometry. Following a dose of 1 mg doxepin-HCl/kg intravenously (i.v.), both the isomers were quantified for up to 24 h in serum of horses (n=4). The beta-half-lives of the trans- and cis-isomers were 3.5 and 3.1 h, respectively. The ratio of the trans/cis-isomers was found to be constant (4.7:1) during drug elimination and thus corresponded to the original composition of the antidepressant. Up to 12 h following administration low trans-isomer concentrations in an average range of 2-6 ng/mL were detected in urine of each of the horses, while the cis-isomer was only present in two of four horses for up to 8 and 12 h, respectively. In serum, mean trans-isomer concentrations exceeded urine levels maximally 120-fold after 3 h and at least sixfold after 12 h. As serum exhibits considerably higher concentrations of the doxepin isomers as compared with urine, blood of horses is the recommended body fluid when screening for the antidepressant.

show abstract

Plasma levels and tricyclic antidepressant therapy: Part I. A review of assay methods

Gupta¹,

Molnár²

1980

Biopharm & Drug Disp

View full text Add to dashboard Cite

Quantitative determination of doxepin and desmethyldoxepin in rat plasma by means of gas-liquid chromatography-mass fragmentography

Cited by 14 publications

References 7 publications

Quantitative GLC Determination of cis- and trans-Isomers of Doxepin and Desmethyldoxepin

Quantitative GLC Determination of cis- and trans-Isomers of Doxepin and Desmethyldoxepin

Elimination of doxepin isomers from the horse following intravenous application

Plasma levels and tricyclic antidepressant therapy: Part I. A review of assay methods

Contact Info

Product

Resources

About