Quantitative Determination of Eight Major Constituents in the Traditional Chinese Medicinal Yi-Qi-Fu-Mai Preparation by LC

Zhou, Duo; Shou-jun, Jiang; Tong, Ling; Yuewu, Yang; Wang, Gang-Li; Ye, Zhengliang; Wang, Zhengtao; Lin, Rui‐Chao

doi:10.1365/s10337-009-1252-3

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…According to our previous report (Zhou et al ., 2009), ginsenoside F 2 and Rg 3 are the main constituents in YQFM injection. While in this chromatographic condition, the two peaks were partially overlapped, nine ginsenosides were chosen as marker compounds.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously reported an LC method (Zhou et al ., 2009) for simultaneous determination of eight markers in YQFM injection. However, it has some limitations including a long analysis time of 65 min and insufficient sensitivity, which may not meet the requirement for high throughput, speed and sensitivity in biological specimen analysis of pharmacokinetic study.…”

Section: Introductionmentioning

confidence: 99%

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An LC‐MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study

Zhou

Tong

Wan

et al. 2010

Biomedical Chromatography

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A sensitive and reliable LC-ESI-MS method for simultaneous determination of nine ginsenosides (Rh(1), Rg(2), Rg(1), Rf, Re, Rd, Rc, Rb(2) and Rb(1)) in rat plasma was developed and validated using saikosaponin A as an internal standard. The samples were extracted by solid-phase extraction. Chromatographic separation was carried out on a Hypersil Gold C(18) column (100 × 2.1 mm, 5 µm) by stepwise gradient elution with water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Detection was determined by selective ion monitoring mode using electrospray ionization in the negative ion mode. Good linearity over the investigated concentration ranges was observed with the values of r higher than 0.9900. The intra- and inter-day precisions were all no more than 15% and the average recoveries varied from 71.8 to 91.7%. This quantitative measurement was successfully applied to a pharmacokinetic study of Yi-Qi-Fu-Mai injection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An LC‐MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study

Zhou

Tong

Wan

et al. 2010

Biomedical Chromatography

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“…The storage solutions of 10 compounds were set up the regression equations, which were plotted after linear regression between the peak areas and concentrations of 10 compounds [18]. The limit of detection (LOD) and quantification (LOQ) were determined at signal-to-noise ratios of 3 and 10, respectively [19,20].…”

Section: Validation Of Uplc Methodsmentioning

confidence: 99%

Simultaneous determination of ten compounds in two main medicinal plant parts of Tibetan herb, <i>Pterocephalus hookeri</i> (CB Clarke) Höeck, by ultra-high performance liquid chromatography-photodiode array

Tang¹,

Fan²,

Li³

et al. 2017

Trop. J. Pharm Res

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“…A large number of literature studies have shown that ginsenosides, lignans and ophiopogonins are the major bioactive constituents in SMS and some of its modern preparations [1,2,7,8,9]. Many researchers have studied the ginsenosides and lignans in YQFM or other Sheng-mai preparations using HPLC-UV/MS or near infrared spectroscopy [10,11,12,13,14], but few of the studies have focused on analysis of ophiopogonins in YQFM. O. japonicus , which contains ophiopogonins, is an essential component of SMS, conferring better biological activity in terms of cardiovascular diseases [15,16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Qualitative and Quantitative Analysis of Multiple Chemical Constituents in YiQiFuMai Injection by Ultra-Fast Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry

Liu

Dazheng

et al. 2016

Molecules

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YiQiFuMai injection (YQFM) is a modern lyophilized powder preparation derived from the traditional Chinese medicine Sheng-mai san (SMS) used for treating cardiovascular diseases, such as chronic heart failure. However, its chemical composition has not been fully elucidated, particularly for the preparation derived from Ophiopogon japonicus. This study aimed to establish a systematic and reliable method to quickly and simultaneously analyze the chemical constituents in YQFM by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Sixty-five compounds in YQFM were tentatively identified by comparison with reference substances or literature data. Furthermore, twenty-one compounds, including three ophiopogonins, fifteen ginsenosides and three lignans were quantified by UFLC-IT-TOF/MS. Notably, this is the first determination of steroidal saponins from O. japonicus in YQFM. The relative standard deviations (RSDs) of intra-and inter-day precision, reproducibility and stability were <4.9% and all analytes showed good linearity (R 2 ě 0.9952) and acceptable recovery of 91.8%-104.2% (RSD ď 5.4%), indicating that the methods were reliable. These methods were successfully applied to quantitative analysis of ten batches of YQFM. The developed approach can provide useful and comprehensive information for quality control, further mechanistic studies in vivo and clinical application of YQFM.

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Quantitative Determination of Eight Major Constituents in the Traditional Chinese Medicinal Yi-Qi-Fu-Mai Preparation by LC

Cited by 7 publications

References 19 publications

An LC‐MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study

An LC‐MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study

Simultaneous determination of ten compounds in two main medicinal plant parts of Tibetan herb, <i>Pterocephalus hookeri</i> (CB Clarke) Höeck, by ultra-high performance liquid chromatography-photodiode array

Simultaneous Qualitative and Quantitative Analysis of Multiple Chemical Constituents in YiQiFuMai Injection by Ultra-Fast Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry

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