Quantitative determination of endogenous sorbitol and fructose in human erythrocytes by atmospheric-pressure chemical ionization LC tandem mass spectrometry

Quantitative analysis of endogenous compounds

“…Various approaches ( Figure 2) are followed to address the lack of blank matrices for the quantification of endogenous compounds by LC-MS/MS including, background subtraction [26-31], the standard addition method [24,25,[32][33][34][35][36][37][38][39][40][41][42][43][44], neat solutions [45][46][47][48][49][50][51][52], artificial matrices of biological fluids [23,, stripped matrices [8,10,11,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88][89][90][91], and surrogate analytes [66,[92][93][94][95][96][97]…”

Prophylactic Medical Treatment of Diabetic Retinopathy

“…Therefore, it is important to find medical options other than glycemic control to prevent diabetic ocular complications. The metabolic changes that accompany hyperglycemia, such as activation of the polyol pathway (Gabbay, 1973;Lorenzi, 2007;Robison et al, 1988;, activation of protein kinase C (PKC) (Frank, 2002;Liang et al, 2005;Shiba et al, 1993), increased oxidative stress (Gurler et al, 2000;Jennings et al, 1991;Pan et al, 2008;Pinto et al, 2007), leukocyte adhesion to the endothelial cells (McLeod et al, 1995;Miyamoto et al, 1996;Schroder et al, 1991), and accumulation of advanced glycation end products (AGEs) 292 (Chibber et al, 1997;Hirata et al, 1997;Kakehashi et al, 2008), are related to the development and progression of diabetic ocular complications. In particular, the polyol pathway is correlated strongly with oxidative stress, activation of PKC, and accumulation of AGEs that lead to induction of vascular endothelial growth factor (VEGF).…”

HPLC with pulsed amperometric detection for sorbitol as a biomarker for diabetic neuropathy

“…Typical GC-MS methods require derivatization steps to increase volatility, and can also exhibit sorbitol interferences including glucose, fructose, and other polyols, thereby requiring the removal of other sugars and sugar alcohols before analysis [7]. LC/APCI-MS/MS also has disadvantages, such as a long pretreatment time and the need for expensive equipment [8].…”