Quantitative determination of hyoscyamine and scopolamine by direct photodensitometry of thin-layer chromatograms

Chu, Bao-Lin; Mika, Edward S.; Solomon, Mark J.; Crane, F.A.

doi:10.1002/jps.2600580907

Cited by 18 publications

(2 citation statements)

References 15 publications

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“…a) These figures were taken from references [171(18] [19]. b) Fifteen milligrams (dry weight) of Hi 5 cells were cultured on a slow reciprocal shaker in the dark for 5 weeks in a 50-mI Erlenmeyer flask with 10 ml of medium.…”

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Scopolamine Production in Suspension Cultures and Redifferentiated Roots ofHyoscyamus niger

Hashimoto¹,

Yamada²

1983

Planta Med

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Adventitious roots were induced from 2-year-old, hyoscyamine rich suspension cultures of Hyoscyamus niger L. The cultured roots grew well in Linsmaier-Skoog medium containing 10 (-8)M 6-benzyladenine with 3% sucrose and produced much more scopolamine (0.12-0.30% dW) than hyoscyamine (0.04-0.08% dW). The alkaloids were detected in the medium as well as in the cells. Ten to twenty percent of the hyoscyamine added to the medium was converted to scopolamine within 24 days by the root cultures, but only 1 to 3% was converted by the suspension cultures. Scarcely any of the scopolamine added to the medium was converted to hyoscyamine by either culture.

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Scopolamine Production in Suspension Cultures and Redifferentiated Roots ofHyoscyamus niger

Hashimoto¹,

Yamada²

1983

Planta Med

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“…Our results were consistant with other studies revealed that TLC is the method of choice for routine alkaloid analysis before further sophisticated instrumental analysis is performed. It is considered simple and rapid method for the quantitative evaluation of hyoscyamine where discrete spots were quickly obtained by spraying with modified dragendorf's reagent (Chu et al, 1969;Christen et al, 2013;Kokotkiewicz et al, 2017). Presence of hyoscyamine in the endophytic fungi differs according to type of used growth media (SDB, PDB and RG) which affected alkaloids content especially hyoscyamine in each fungus.…”

Section: Detection Of Various Fungal Secondary Metabolitesmentioning

confidence: 99%

Bioprospecting of Hyoscyamine Alkaloid and Other Secondary Metabolites Production by Some Fungal Endophytes Isolated from Hyoscyamus muticus

El-Debaiky,

El-Somany,

Shoeib

et al. 2023

Egypt. J. Bot.

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H YOSCYAMUS muticus L. (Egyptian Henbane), a shrub thriving in the sandy regions of Egypt, shows important pharmacological properties which may be attributed to its own bioactive compounds or due to associated endophytes. In the present study, roots, stems and leaves of H. muticus growing in Egypt were evaluated for the presence of associated fungal endophytes, and secondary metabolites produced from them especially the characteristic plant tropane alkaloid; hyoscyamine. Using culture-dependent approach, sixteen fungal endophytes belonging to five different genera were identified morphologically according to their culture appearance and detailed microscopic structures. The isolated endophytic fungi were cultured using three different media (PDB, RG and SDB) and each filtered broth was sequentially extracted with ethyl acetate. Crude extracts collected from different fungal isolates were subjected to phytochemical analysis using thin layer chromatography (TLC) which showed a distinct fingerprinting profile of several fungal secondary metabolites. The total secondary metabolite, phenolic compound, flavonoid, anthraquinone glycoside, coumarins and alkaloid content was investigated. Different activities of the used media were reported on the changing secondary metabolic profile of each fungus. Furthermore, the ability of the tested fungal endophytes to produce hyoscyamine, characteristic to H. muticus was tested by TLC analysis. Some isolated endophyte species belonging to Aspergillus and Penicillium showed positive tests for hyoscyamine.

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Sensitive HPLC determination of atropine with electrochemical detection

Leroy

Moreau

Nicolas

et al. 1983

J. High Resol. Chromatogr.

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Quantitative determination of hyoscyamine and scopolamine by direct photodensitometry of thin-layer chromatograms

Cited by 18 publications

References 15 publications

Scopolamine Production in Suspension Cultures and Redifferentiated Roots ofHyoscyamus niger

Scopolamine Production in Suspension Cultures and Redifferentiated Roots ofHyoscyamus niger

Bioprospecting of Hyoscyamine Alkaloid and Other Secondary Metabolites Production by Some Fungal Endophytes Isolated from Hyoscyamus muticus

Sensitive HPLC determination of atropine with electrochemical detection

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