Sensitive HPLC determination of atropine with electrochemical detection

Leroy, Pierre; Moreau, A.; Nicolas, Alain; Hosotte, B.

doi:10.1002/jhrc.1240060915

Cited by 7 publications

(4 citation statements)

References 6 publications

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“…Some analyses of histidine and histidine‐rich proteins have been developed in conjunction with immunoassay2a–d and colorimetric detection methods 2e. The most commonly used method for the detection of histidine and histidine‐rich proteins in biological samples is chromatography, which is usually performed through the combination of an effective separation technique, such as thin‐layer chromatography, gas chromatography, or HPLC, followed by UV/Vis or fluorescence spectroscopy 2f–h. The use of high‐performance capillary electrophoresis with a derivation reagent has also been reported 2i.…”

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confidence: 99%

A Highly Selective Luminescent Switch‐On Probe for Histidine/Histidine‐Rich Proteins and Its Application in Protein Staining

et al. 2008

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The luminescence sensing of histidine and histidine-rich proteins plays a pivotal role in biochemistry and molecular biology, in particular when both temporal and spatial resolution are required. An abnormal level of histidine-rich proteins is an indicator for many diseases, such as advanced liver cirrhosis, [1a,b] AIDS, [1c] renal disease, [1c] asthma, [1c] pulmonary disorders, [1d] thrombotic disorders, [1e, f] and malaria.[1g]Some analyses of histidine and histidine-rich proteins have been developed in conjunction with immunoassay [2a-d] and colorimetric detection methods.[2e] The most commonly used method for the detection of histidine and histidine-rich proteins in biological samples is chromatography, which is usually performed through the combination of an effective separation technique, such as thin-layer chromatography, gas chromatography, or HPLC, followed by UV/Vis or fluorescence spectroscopy.[2f-h] The use of high-performance capillary electrophoresis with a derivation reagent has also been reported.[2i] However, the aforementioned methods are generally tedious, laborious, and, most importantly, expensive for routine detection in a biochemistry laboratory.Although numerous studies have dealt with the detection of histidine or histidine-rich proteins, studies on the use of luminescent probes for this purpose remain sparse.[3] Notable examples include research by Fabbrizzi and co-workers, who developed competitive noncovalent fluorescence turn-on probes for histidine in the form of dizinc(II) or dicopper(II) macrocyclic complexes, which recognize histidine through the formation of an imidazolate bridge between the two dizinc(II) or dicopper(II) centers; [3a] however, the resulting noncovalent ensemble may be less stable than a covalently linking sensory system, and the complexity of the synthetic process makes it difficult to implement in a convenient manner.Photoluminescent iridium(III) complexes have emerged as a topical area of interest in inorganic photochemistry [4] and phosphorescent materials for optoelectronic [5] and luminescence signaling applications.[6] Significant changes in the photophysical behavior and emission properties of iridium-(III) complexes may be induced by the presence of biomolecules. Luminescent transition-metal complexes for protein staining, such as the luminescent ruthenium complex known as SYPRO Ruby dye, have been reported previously. [7] However, despite its high sensitivity and its broad dynamic range, the use of SYPRO Ruby dye is limited, as it is sold only as a formulated solution; therefore, it is not possible to optimize the dye for a particular electrophoresis protocol and protein. + , and the iridium complex is readily soluble and stable in aqueous staining solutions. In this study, [Ir(ppy) 3 ] (2) was also prepared for comparative studies, as its binding with proteins was expected to be largely hydrophobic in nature. Herein, we describe the luminescent switch-on probe [Ir(ppy) 2 (solv) 2 ] + (1) for histidine/histidinerich proteins and demonstrate...

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A Highly Selective Luminescent Switch‐On Probe for Histidine/Histidine‐Rich Proteins and Its Application in Protein Staining

et al. 2008

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“…Chromatographic methods, including high performance liquid chromatography (HPLC) [6][7][8][9][10][11] and gas chromatography (GC), [12][13][14] require an extensive clean-up procedure in addition to their expensive instrumentation, especially when mass spectrometer detectors are used. The reported thin layer chromatography (TLC) [15] method involved extraction of the sample with ether at an alkaline pH, followed by reaction of the extracted HTM with 4-chloro-7-nitrobenzo-2,1,3-oxadiazole before the separation step.…”

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confidence: 99%

Orthophthaldehyde as a fluorescent probe for the feasible and sensitive assay of heptaminol: application to dosage forms and real human plasma

et al. 2021

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The antihypotensive drug heptaminol was determined using a spectrofluorimetric method and ortho-phthaladehyde as a fluorescence probe. The drug was mixed with the reagent in the presence of 2-mercaptoethanol and the reaction was carried out in slightly alkaline aqueous solution containing 0.1 M sodium hydroxide. The resulting product exhibited high fluorescence activity that was measured at 451 nm after excitation at 334 nm. The linearity range of the method was 5-100 ng ml À1 with a lower detection limit of 1.8 ng ml À1 . The procedure was evaluated according to the International Council of Harmonization guidelines. The proposed method was applied to analyze the drug in pharmaceutical tablets and oral drops. In addition, the present study represents the first spectrofluorimetric method for the determination of the cited drug in real human plasma. The method provided high recovery percentages without any interference from coexisting pharmaceutical excipients or the components of human plasma.

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“…[1] Various methods have been reported for determining heptaminol in its dosage forms and in plasma. Spectrophotometric [2][3][4] , spectrofluorimetric [4][5][6][7][8] , chromatographic [9][10][11][12][13][14][15][16][17][18][19][20][21][22]] and capillary electrophoretic [23]…”

Section: Introductionmentioning

confidence: 99%

Condensation of heptaminol hydrochloride for its spectrofluorimetric determination in pure form and tablets: application in human plasma

2020

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A sensitive, simple, accurate and less expensive fluorimetric method was designed and validated for analysis of heptaminol HCl in both its pure and dosage forms, as well as in human plasma. The main principle used in the proposed approach was the condensation reaction between heptaminol's primary amino moiety and ethyl acetoacetate/formaldehyde reagents, giving a derivative that was highly fluorescent at 416 nm after excitation at 350 nm. Various experimental parameters that affected either the product's development or its stability were evaluated and optimized. The constructed calibration curve was linear over the range 0.2–2 μg/ml, with a good correlation coefficient (0.9996). Both the calculated limit of detection and limit of quantitation were 0.06 and 0.18 μg/ml, respectively. The presented approach was a success when used to determine Corasore® tablets and was validated according to International Council for Harmonisation guidelines.

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Sensitive HPLC determination of atropine with electrochemical detection

Cited by 7 publications

References 6 publications

A Highly Selective Luminescent Switch‐On Probe for Histidine/Histidine‐Rich Proteins and Its Application in Protein Staining

A Highly Selective Luminescent Switch‐On Probe for Histidine/Histidine‐Rich Proteins and Its Application in Protein Staining

Orthophthaldehyde as a fluorescent probe for the feasible and sensitive assay of heptaminol: application to dosage forms and real human plasma

Condensation of heptaminol hydrochloride for its spectrofluorimetric determination in pure form and tablets: application in human plasma

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